Method for preparing injectable porous hydrogel microspheres by microfluidic ice crystal method and application of injectable porous hydrogel microspheres

Abstract

The invention provides a method for preparing injectable porous hydrogel microspheres by a microfluidic ice crystal method and application of the injectable porous hydrogel microspheres, and the preparation method comprises the following steps: (1) mixing a hydrogel material, a buffer solution and a photoinitiator to obtain a hydrogel solution, and adding dimethyl sulfoxide into the hydrogel solution to obtain an aqueous phase solution; mixing an oily material with a surfactant to obtain an oil phase solution; (2) adding the aqueous phase solution and the oil phase solution into a microfluidicdevice to prepare hydrogel micro-droplets; (3) carrying out low-temperature treatment on the hydrogel micro-droplets to form ice crystals; after the ice crystals are completely formed, maintaining alow-temperature environment, carrying out curing cross-linking under ultraviolet irradiation, and heating to room temperature after cross-linking is completed, so that pores are formed after the ice crystals are melted, and the porous hydrogel microspheres are obtained. The hydrogel microsphere disclosed by the invention has the characteristics of being porous and uniform in pore diameter, can load different types of cells, and has a very great application prospect in the aspects of bone repair, skin defects and the like as an excellent cell carrier.

Description

technical field [0001] The invention belongs to the technical field of preparation of hydrogel materials, and in particular relates to a method for preparing injectable porous hydrogel microspheres by a microfluidic ice crystal method and an application thereof. Background technique [0002] Hydrogel is a three-dimensional network structure containing a large amount of water formed by the cross-linking of hydrophilic polymers. It has the advantages of high hydrophilicity, high viscoelasticity, low friction coefficient, and excellent biocompatibility. Adhesive materials are widely used in biomedical fields such as cell carriers, drug carriers, and tissue engineering. Generally, the construction materials of hydrogels are mainly divided into two types: natural polymers and synthetic polymers. Among them, natural polymer materials refer to polymer materials derived from natural animals and plants, which have excellent biocompatibility and biological activity, mainly including ...

AI Technical Summary

Problems solved by technology

However, the currently commonly used hydrogel microspheres have the following problems: (1) lack of pores inside the microspheres, resulting in impermeability of nutrients and the inability of internal cells to survive; (3) A chemical cross-linking agent is used in the preparation process, and its degradation products have certain toxicity to the body

[0004] At present, the methods commonly used to prepare porous hydrogels are mainly freeze-drying, particle leaching and air bubble methods. However, these methods have changes in the external structure of the material, pores The size is uncontrollable, only suitable for bulk hydrogels and not suitable for the preparation of micron-sized hydrogel microspheres, etc.

Images