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Ralstonia pickettii EZ-50, metabolite and application thereof

A technology of EZ-50 and metabolites, applied in the fields of application, bacteria, and microorganisms, can solve the problems of unclear microbial strains such as Rhizoctonia solani, achieve excellent effects, reduce pesticide application, and ensure agricultural health and sustainability The effect of development

Active Publication Date: 2021-02-26
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to the complexity and variability of microorganisms in nature, as well as the wide variety and large amount of mVOC secreted by them, it is not clear whether there are more effective microbial strains in nature and their metabolic mVOC can inhibit the growth of Rhizoctonia solani

Method used

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  • Ralstonia pickettii EZ-50, metabolite and application thereof
  • Ralstonia pickettii EZ-50, metabolite and application thereof
  • Ralstonia pickettii EZ-50, metabolite and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1: Screening and Identification of Biocontrol Strains

[0024] (1) screening

[0025] In December 2018, Wang Enzhao collected healthy rice rhizosphere soil samples in a pot experiment in the greenhouse of the Chinese Academy of Agricultural Sciences (remove about 5 cm of the covering soil on the root surface from the rice, and randomly collect the loose soil combined with the rice root system in an S-shape).

[0026] The specific operation method of the test group is as follows:

[0027] Use the gradient dilution coating method to apply the suspension of the soil sample diluted step by step on the beef extract peptone plate, pick a single colony into the liquid beef extract peptone medium to obtain the bacterial liquid, and take each bacterial liquid and spread it evenly on the beef extract peptone plate. Cultivate on paste-peptone medium for 24 h, inoculate 10 uL of Rhizoctonia solani bacteria solution in the center of the inoculated plate (Rhizocustonia solan...

Embodiment 2

[0040] Example 2: Identification and functional verification of antibacterial metabolites of Ralstonia piteri

[0041] Volatile organic compounds are solids or liquids with carbon as the basic element, which can quickly volatilize at 20°C and 0.01 kPa and enter the gas phase. In view of the long-term research results accumulated by the inventors in the early stage, such microorganisms can produce a large number of volatile organic compounds with rich types and diverse functions. The main functions of these substances are: as signal substances between communities and within communities; as signal substances between cells ; possible carbon release pathways; growth-promoting and growth-inhibiting factors. If they function as inhibitors and the phytopathogenic microorganisms are inhibited in the interaction, then the microorganisms releasing volatile organic compounds have the potential to be applied to the biological control of plant diseases.

[0042] (1) Collection of volatile...

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Abstract

The invention discloses Ralstonia pickettii EZ50, metabolite and application thereof, and aims to solve the technical problem of poor prevention and treatment effect on rice sheath blight disease in the prior art. The Ralstonia pickettii CGMCC NO.20622 disclosed by the invention can strongly inhibit rhizoctonia solani, and can be used for preventing and treating plant diseases caused by rhizoctonia solani, so that the purpose of reducing pesticide application is achieved, the sustainable development of agriculture is ensured, and the ralstonia pickettii has a wide application prospect. The Ralstonia pickettii disclosed by the invention can metabolize and secrete a plurality of bacteriostatic active substances, including ethyl benzoate, 6-methyl-5-heptene-2-ketone, 2-ethyl-1-hexanol and dimethyl disulfide, and the volatile substances have a good effect of inhibiting rhizoctonia solani.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to Ralstonia pickettii (Ralstonia pickettii) EZ-50, its metabolites and applications thereof. Background technique [0002] Rice is an important staple food crop in the world, and more than 3.5 billion people need rice for energy every day. Rice sheath blight caused by Rhizoctonia solani is one of the most important rice diseases worldwide, causing devastating crop losses and seriously threatening global food security. At present, fungicides are mainly used in agricultural production to control the occurrence and development of rice sheath blight. For example, Jinggangmycin has been widely used to control sheath blight in China over the past 30 years. However, continuous high-volume use of a single fungicide greatly increases the risk of pathogens developing resistance. Breeding resistant varieties is the best option to deal with the pathogen attack, but so far, there ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/62C12P7/26C12P7/04C12P11/00A01N63/20A01N37/10A01N35/02A01N31/02A01N41/12A01P3/00A01P21/00C12R1/01
CPCC12P7/62C12P7/26C12P7/04C12P11/00A01N63/20A01N37/10A01N35/02A01N31/02A01N41/12C12R2001/01C12N1/205A01P3/00
Inventor 宋阿琳范分良王恩召
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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