A fermented feed additive with immune enhancing effect and preparation method thereof
A technology of fermented feed and immune enhancement, applied in biochemical equipment and methods, animal feed, animal feed, etc., can solve problems such as digestive system disorders, improve anti-inflammatory and antibacterial ability, increase yield, and improve body immunity Effect
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Embodiment 1
[0032] (1) Mix 16g Astragalus, 12g Honeysuckle, 8g Chine Neijin and 32g wheat bran, pass through a 40-60 mesh sieve after pulverizing, the mixture obtained, the mixture is mixed with water to a water content of 45%, and packed into a 500mL conical flask , 121 ℃, sterilization 30min, get culture medium.
[0033] (2) Take 100 g of medium, and add 0.55 g of cellulase and 0.2 g of neutral protease into the medium, respectively. Enzymatic hydrolysis was carried out at 37°C for 5h, and the enzyme was inactivated at 80°C for 30min to obtain the enzymatic hydrolysate;
[0034] (3) Take 100 g of enzymatic hydrolysate, add citric acid to the enzymatic hydrolysate to adjust the pH to 6.5, add 2.25 mL of previously cultivated Bacillus subtilis bacterial solution and 2.25 mL of Hansenula yeast bacterial solution, and the fermentation temperature is 35°C , and let stand for 18h to obtain a fermented product.
[0035] (4) Put the fermented product into an oven, and dry it at 45° C. until t...
Embodiment 2
[0037] (1) 12g Astragalus root, 16g honeysuckle flower, 4g Chinella japonica and 40g wheat bran are mixed, and after pulverization, pass through a 40-60 mesh sieve, and the mixture obtained, the mixture is mixed with water to a water content of 40%, is loaded into a 500mL conical flask , 121 °C, sterilized for 30 min to obtain culture medium.
[0038] (2) Take 100 g of medium, and add 0.66 g of cellulase and 0.33 g of neutral protease into the medium, respectively. Enzymatic hydrolysis was carried out at 40°C for 4 hours, and the enzyme was inactivated at 80°C for 30 minutes to obtain the enzymatic hydrolysate.
[0039] (3) Take 100 g of the enzymatic hydrolysate, add citric acid to the enzymatic hydrolysate to adjust the pH to 6.8, and add 2.5 mL of the previously cultivated Bacillus subtilis bacterial solution and 2.5 mL of the Hansenula yeast bacterial solution. The fermentation temperature was 36° C., and it was allowed to stand for 16 hours to obtain a fermented product....
Embodiment 3
[0042] (1) 20g of Astragalus, 8g of honeysuckle, 12g of Jin Nei Jin and 24g of wheat bran are mixed, and the obtained mixture is mixed with water to a 50% water content, and is packed into a 500mL conical flask. , 121 ℃, sterilization 30min, get culture medium.
[0043] (2) Take 100 g of medium, and add 0.375 g of cellulase and 0.125 g of neutral protease into the medium, respectively. Enzymatic hydrolysis was carried out at 35 °C for 6 h, and the enzyme was inactivated at 80 °C for 30 min to obtain the enzymatic hydrolysate.
[0044] (3) Take 100 g of the enzymatic hydrolysate, add citric acid to the enzymatic hydrolysate, adjust the pH to 6.0, and add 2.0 mL of the previously cultivated Bacillus subtilis bacterial solution and 2.0 mL of the Hansenula yeast bacterial solution. The fermentation temperature was 34°C, and it was allowed to stand for 20 hours to obtain a fermented product.
[0045] (4) Put the fermented product into an oven, and dry it at 45° C. until the moist...
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