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Special culture medium for producing concentrated oocystis and culture method thereof

A technology of oocystis and culture medium, which is applied in the field of microalgae cultivation, can solve the problems of low cultivation efficiency, low concentration of algae liquid, influence on use, etc., and achieves the effect of high success rate of expanded cultivation and fast growth rate.

Pending Publication Date: 2021-03-09
武汉市中易天地物联科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] During the cultivation process of existing oocysts, most of them are self-supporting light cultivation, the cultivation efficiency is low, and the concentration of the cultured algae liquid is low, which affects the use

Method used

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  • Special culture medium for producing concentrated oocystis and culture method thereof
  • Special culture medium for producing concentrated oocystis and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: A kind of special medium for producing concentrated oocysts, including A component and B component, A component includes glucose 15g / L, sodium acetate 5g / L, sodium nitrate 5g / L, urea 1g / L, Ammonium Chloride 2g / L, Sodium Dihydrogen Phosphate Dihydrate 0.1g / L, Disodium EDTA 0.1g / L, Ferric Chloride Hexahydrate 0.2g / L. The preparation method of component A is as follows: take each substance in component A, dissolve it in water in a fermenter in proportion, adjust the pH with NaOH solution within the range of 7.5±0.2, and sterilize at 121°C for 20 minutes.

[0020] Component B includes citric acid 3g / L, ferric ammonium citrate g / L, calcium chloride dihydrate 20g / L, manganese chloride tetrahydrate 0.5g / L, zinc sulfate heptahydrate 0.2g / L, molybdic acid dihydrate Sodium 0.3g / L, copper sulfate pentahydrate 0.5g / L, cobalt nitrate hexahydrate 0.3g / L, vitamin B1 0.1g / L, vitamin B12 0.001g / L, biotin 0.003g / L. The preparation method of component B is as follows, take...

Embodiment 2

[0023] Embodiment 2: A kind of special medium for producing concentrated oocysts, including A component and B component, A component includes glucose 55g / L, sodium acetate 10g / L, sodium nitrate 10g / L, urea 3g / L, Ammonium Chloride 5g / L, Sodium Dihydrogen Phosphate Dihydrate 0.4g / L, Disodium EDTA 0.3g / L, Ferric Chloride Hexahydrate 0.4g / L. The preparation method of component A is as follows: take each substance in component A, dissolve it in water in a fermenter in proportion, adjust the pH with NaOH solution, maintain it within the range of 7.5±0.2, and sterilize at 121°C for 20 minutes.

[0024] Component B includes citric acid 8g / L, ferric ammonium citrate 8g / L, calcium chloride dihydrate 50g / L, manganese chloride tetrahydrate 3g / L, zinc sulfate heptahydrate 0.6g / L, sodium molybdate dihydrate 0.8g / L, copper sulfate pentahydrate 0.8g / L, cobalt nitrate hexahydrate 0.6g / L, vitamin B1 0.3g / L, vitamin B12 0.01g / L, biotin 0.005g / L. The preparation method of component B is as foll...

Embodiment 3

[0027] Embodiment 3: A kind of special medium for producing concentrated oocysts, the difference from Example 1 is that when the special medium for producing concentrated oocysts is used to cultivate oocysts, each material in the A component is taken , dissolve in water in proportion in the fermenter, adjust the pH with NaOH solution, maintain it within the range of 7.5±0.2, sterilize at 121°C for 20min, cool to 30°C, add the filter-sterilized component B into the fermenter , inoculating the oocyst seed liquid in a fermenter, cultivating at 25-35° C., cultivating until the fermentation biomass reaches 35000-50000 mg / L, and ending the fermentation to obtain concentrated oocysts.

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Abstract

The invention relates to the technical field of microalgae culture, and discloses a special culture medium for producing concentrated oocystis. The special culture medium comprises a component A and acomponent B, the component A comprises glucose, sodium acetate, sodium nitrate, urea, ammonium chloride, sodium dihydrogen phosphate dihydrate, disodium EDTA and ferric chloride hexahydrate, and thecomponent B comprises citric acid, ammonium ferric citrate, calcium chloride dihydrate, manganese chloride tetrahydrate, zinc sulfate heptahydrate, sodium molybdate dihydrate, copper sulfate pentahydrate, cobalt nitrate hexahydrate, vitamin B1, vitamin B12 and biotin. The special culture medium has the advantages that an oocystis seed solution is inoculated after the component A and the componentB are mixed, the oocystis can rapidly grow during fermentation culture, the concentrated oocystis solution is produced, and the oocystis culture medium has the effects of high growth rate and high success rate of enlarged culture. Glycerin, maltotriose and glycine are added into the special culture medium, and in the culture process, the oocystis with an obvious inhibition effect on moss can be obtained through domestication under the induction of various factors.

Description

technical field [0001] The invention relates to the technical field of microalgae cultivation, in particular to a special medium for producing concentrated oocysts and a cultivation method thereof. Background technique [0002] With the use of a large amount of chemical fertilizers in agricultural production and the discharge of industrial wastewater, a large amount of nitrogen and phosphorus elements enter the water body, making the environment of the aquaculture water body increasingly eutrophic, and bad algae in the aquaculture water body such as blue-green algae, moss, and blue-green algae often have rapid The ability to grow makes the aquaculture water body often have cyanobacteria blooms or other bad algal phases, leading to the deterioration of the aquaculture water environment, poisoning of the aquaculture animals, and ultimately the failure of aquaculture. [0003] In the prior art, beneficial algae preparations are used to artificially intervene in the algae phase ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C02F3/32C12R1/89C02F103/20
CPCC12N1/12C02F3/322C02F2103/20
Inventor 唐宾国谢敏代中艳王继岩
Owner 武汉市中易天地物联科技有限公司
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