Lactobacillus gasseri and application thereof in relieving and treating hyperuricemia

A Lactobacillus gasseri, hyperuricemia technology, applied in the field of microorganisms, can solve problems such as undiscovered, and achieve the effects of promoting health, promoting excretion, and reducing gout

Active Publication Date: 2021-03-09
JIANGNAN UNIV
View PDF12 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The excretion of uric acid in the human body is mainly through the excretion of the kidneys and intestines. As a uric acid transporter, ABCG2 plays an important role in the intestinal excretion of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus gasseri and application thereof in relieving and treating hyperuricemia
  • Lactobacillus gasseri and application thereof in relieving and treating hyperuricemia
  • Lactobacillus gasseri and application thereof in relieving and treating hyperuricemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Screening of Lactobacillus gasseri CCFM1133

[0044] (1) Isolation and screening of Lactobacillus

[0045] (1) Take 1g of fresh feces of healthy adults. After gradient dilution, it was applied to LBS medium supplemented with 1% nystatin, and placed in a constant temperature incubator at 37°C for 48 hours.

[0046] (2) After cultivation, according to the color, size, and edge shape of the colony, use an inoculation loop to pick up the colony and streak it for purification.

[0047] (3) The obtained colonies were subjected to Gram staining and catalase analysis.

[0048] (4) Retain Gram-positive bacilli and catalase-negative bacteria.

[0049] (2) Molecular biological identification of Lactobacillus

[0050] (1) Single bacterial genome extraction

[0051] (A) cultivating the lactobacilli screened in step (1) overnight;

[0052](B) Take 1 mL of the bacterial suspension cultivated overnight in a 1.5 mL centrifuge tube, centrifuge at 10000 r / min for 2 min, di...

Embodiment 2

[0069] Example 2: Lactobacillus gasseri CCFM1133 has good tolerance to simulated gastrointestinal fluid

[0070] The cryopreserved Lactobacillus gasseri CCFM1133 was inoculated in MRS medium, anaerobically cultured at 37° C. for 14 hours, and subcultured in MRS medium for 2 to 3 times.

[0071] Take 3mL of the culture solution of Lactobacillus gasseri CCFM1133 and centrifuge at 8000×g for 2min to collect the bacteria, mix it with 3mL pH 3.0 artificial simulated gastric juice (containing 3g / L pepsin, pH=3.0 physiological saline), and anaerobically Cultivate, take samples at 0h and 2h respectively, use MRS agar medium to pour culture, count the colonies on the plate, determine the number of viable bacteria and calculate their survival rate.

[0072] Take 3 mL of the culture solution of Lactobacillus gasseri CCFM1133 and centrifuge at 8000×g for 2 min to collect the cells, add 3 mL of artificial simulated intestinal fluid (containing trypsin 1 g / L, bile salt 0.3%, pH=8.0) to mix,...

Embodiment 3

[0076] Example 3: Lactobacillus gasseri CCFM1133 has no toxic side effects on KunMing mice

[0077] Suspend Lactobacillus gasseri CCFM1133 in a sucrose solution with a concentration of 30g / L to make a concentration of 4.0×10 9 CFU / mL bacterial suspension. Twelve healthy male KunMing mice with a body weight of about 38-44 g were taken, and after one week of adaptation to the environment, they were divided into CCFM1133 group and control group. The CCFM1133 group was given this concentration of bacterial suspension 0.3mL by intragastric administration once a day, and the control group was intragastrically administered the same amount of 30g / L sucrose solution without Lactobacillus gasseri CCFM1133, observed for one week, and recorded death and body weight.

[0078] The results of these tests are listed in Table 2. These results indicate that feeding concentrations of 1×10 9 CFU / Lactobacillus gasseri CCFM1133 had no significant impact on mice, no significant change in body wei...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses lactobacillus gasseri and application thereof in relieving and treating hyperuricemia, and belongs to the technical field of microorganisms. According to the lactobacillus gasseri CCFM1133 disclosed by the invention, the serum uric acid level of hyperuricemia mice and the xanthine oxidase (XOD) activity of serum and a liver can be reduced, and the occurrence of hyperuricemia and gout is reduced; the blood glucose and serum triglyceride (TG) level of a patient with hyperuricemia can be regulated, and the activity of liver catalase (CAT) and glutathione peroxidase (GSH-Px) is improved; expression of ileum ABCG2 is improved, and excretion of intestinal uric acid is promoted; and the intestinal short-chain fatty acid level is improved, and the health is promoted. The lactobacillus gasseri CCFM1133 disclosed by the invention can be used for preparing foods, functional foods or medicines, and has a wide application prospect.

Description

technical field [0001] The invention relates to a strain of Lactobacillus gasseri and its use for alleviating and treating hyperuricemia, belonging to the technical field of microorganisms. Background technique [0002] Hyperuricemia (Hyperuricemia, HUA) refers to a disease in which the level of uric acid in the blood exceeds the normal value. In recent years, with the improvement of living standards, the incidence of hyperuricemia is also increasing. In my country, patients with hyperuricemia account for about 13.3% of the total population. Urate stones caused by long-term hyperuricemia can further induce gout. At the same time, hyperuricemia is considered a risk factor for cardiovascular and cerebrovascular diseases, chronic kidney disease, and atherosclerosis, which seriously endangers human health. Therefore, the treatment of hyperuricemia has attracted great attention. At present, the drugs for the treatment of hyperuricemia mainly include allopurinol (xanthine oxidas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/20A61K35/747A61P19/06A23L33/135A23L33/00C12R1/225
CPCC12N1/20A61K35/747A61P19/06A23L33/135A23L33/00A23V2002/00A23V2400/145A23V2200/30
Inventor 王刚倪彩新王琳琳赵建新张灏陈卫
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap