A strain of Lactobacillus gasseri and its use for relieving and treating hyperuricemia

A technology of Lactobacillus gasseri and hyperuricemia, applied in the field of microorganisms, can solve problems such as undiscovered

Active Publication Date: 2022-08-02
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The excretion of uric acid in the human body is mainly through the excretion of the kidneys and intestines. As a uric acid transporter, ABCG2 plays an important role in the intestinal excretion of uric acid. The expression of intestinal ABCG2 is considered to be a new target for the treatment of hyperuricemia and gout. point, but currently no drugs targeting intestinal ABCG2 have been found

Method used

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  • A strain of Lactobacillus gasseri and its use for relieving and treating hyperuricemia
  • A strain of Lactobacillus gasseri and its use for relieving and treating hyperuricemia
  • A strain of Lactobacillus gasseri and its use for relieving and treating hyperuricemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Screening of Lactobacillus gasseri CCFM1133

[0044] (1) Isolation and screening of Lactobacillus

[0045] (1) Take 1g of fresh feces from healthy adults. After gradient dilution, it was coated on LBS medium supplemented with 1% nystatin, and cultured in a constant temperature incubator at 37°C for 48h.

[0046] (2) After culturing, according to the color, size, edge shape, etc. of the colony, pick the colony with an inoculation loop and purify it by streaking.

[0047] (3) The obtained colonies were subjected to Gram staining and catalase analysis.

[0048] (4) Retain Gram-positive bacilli and catalase-negative bacteria.

[0049] (2) Molecular biological identification of Lactobacillus

[0050] (1) Single bacterial genome extraction

[0051] (A) the lactobacillus obtained by step (1) is cultivated overnight;

[0052](B) get 1mL of bacterial suspension cultured overnight in a 1.5mL centrifuge tube, centrifuge at 10000r / min for 2min, discard the supernata...

Embodiment 2

[0069] Example 2: Lactobacillus gasseri CCFM1133 has good tolerance to simulated gastrointestinal fluid

[0070] The cryopreserved Lactobacillus gasseri CCFM1133 was inoculated into MRS medium, cultured anaerobically at 37°C for 14 hours, and then subcultured with MRS medium for 2 to 3 times.

[0071] Take 3 mL of Lactobacillus gasseri CCFM1133 culture solution by centrifugation at 8000 × g for 2 min to collect bacterial cells, mix with 3 mL of pH 3.0 artificial simulated gastric juice (containing 3 g / L pepsin, pH=3.0 normal saline), and anaerobic at 37 ° C Culture, take samples at 0h and 2h respectively, use MRS agar medium for pouring culture to count the plate colonies, measure the number of viable bacteria and calculate the survival rate.

[0072] Take 3 mL of the culture solution of Lactobacillus gasseri CCFM1133 by centrifugation at 8000 × g for 2 min to collect bacterial cells, add 3 mL of pH 8.0 artificial simulated intestinal fluid (physiological saline containing try...

Embodiment 3

[0076] Example 3: Lactobacillus gasseri CCFM1133 has no toxic and side effects on KunMing mice

[0077] Lactobacillus gasseri CCFM1133 was resuspended in a sucrose solution with a concentration of 30 g / L to make a concentration of 4.0 × 10 9 CFU / mL bacterial suspension. Twelve healthy male KunMing mice weighing about 38-44 g were selected and divided into CCFM1133 group and control group after one week of acclimatization. The CCFM1133 group was given 0.3 mL of this concentration of bacterial suspension by gavage once a day, and the control group was given the same amount of 30 g / L sucrose solution without Lactobacillus gasseri CCFM1133 by gavage for one week, and the death and body weight were recorded.

[0078] The results of these tests are listed in Table 2. These results indicate that feeding concentrations of 1 × 10 9 CFU / Lactobacillus gasseri CCFM1133 did not significantly affect the mice, and there was no significant change in body weight and no death. The mice show...

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Abstract

The invention discloses a strain of Lactobacillus gasseri and its application for relieving and treating hyperuricemia, belonging to the technical field of microorganisms. The Lactobacillus gasseri CCFM1133 of the present invention can reduce the level of serum uric acid, the activity of xanthine oxidase (XOD) in serum and liver in hyperuricemia mice, reduce the occurrence of hyperuricemia and gout; and can regulate hyperuricemia The blood glucose and serum triglyceride (TG) levels of patients with diabetes mellitus were improved, and the activities of liver catalase (CAT) and glutathione peroxidase (GSH‑Px) were increased; excretion; and increase intestinal short-chain fatty acid levels to promote health. The Lactobacillus gasseri CCFM1133 of the invention can be used for preparing food, functional food or medicine, and has wide application prospects.

Description

technical field [0001] The invention relates to a strain of Lactobacillus gasseri and its application for relieving and treating hyperuricemia, belonging to the technical field of microorganisms. Background technique [0002] Hyperuricemia (Hyperuricemia, HUA) refers to a disease in which the level of uric acid in the blood exceeds the normal value. In recent years, with the improvement of living standards, the incidence of hyperuricemia is also increasing, and patients with hyperuricemia in my country account for about 13.3% of the total population. Urate stones caused by long-term hyperuricemia can further induce gout. At the same time, hyperuricemia is considered to be a risk factor for cardiovascular and cerebrovascular diseases, chronic kidney disease, and atherosclerosis, which seriously endangers human health. Therefore, the treatment of hyperuricemia has attracted great attention. At present, the main drugs for the treatment of hyperuricemia are allopurinol (xanthi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P19/06A23L33/135A23L33/00C12R1/225
CPCC12N1/20A61K35/747A61P19/06A23L33/135A23L33/00A23V2002/00A23V2400/145A23V2200/30
Inventor 王刚倪彩新王琳琳赵建新张灏陈卫
Owner JIANGNAN UNIV
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