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Wormwood traditional Chinese medicine micro-ecological fermentation preparation and preparation method and application thereof

A technology of traditional Chinese medicine microecology and fermented preparations, which is applied in the field of livestock and poultry breeding, can solve problems such as the combination, content, and safety of microecological preparations, the easy loss of activity of live bacterial preparations, and uneven product quality, so as to maintain intestinal microbiota. Ecological balance, improvement of serum biochemical indicators, and the effect of improving the body's immunity

Pending Publication Date: 2021-03-12
上海植酵盛生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are still several problems in the use of microecological preparations in the livestock and poultry breeding industry: live bacterial preparations are easily affected by intestinal bile acids, hydrochloric acid, etc. Breeding, and finally select the strains with better tolerance for transformation and development; in addition, live bacterial preparations tend to lose their activity during processing, transportation, and storage, so the development of microencapsulation technology has a better effect on the growth and drying of probiotics. Effect, according to the characteristics of the strains to produce stable and efficient probiotics with a high number of viable bacteria; product quality varies, and the combination, content, and safety of probiotics are not clear

Method used

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  • Wormwood traditional Chinese medicine micro-ecological fermentation preparation and preparation method and application thereof
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  • Wormwood traditional Chinese medicine micro-ecological fermentation preparation and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Isolation, Identification and Preservation of Example 1 Bacterial Strains

[0038] 1. Isolation of strains

[0039] The samples from natural fermented wormwood were put into the transport medium, quickly placed in the ice box and sent to the laboratory for strain isolation. After the sauerkraut sample was ground and serially diluted, the Lactobacillus in the sample was isolated using a Lactobacillus selective medium, cultured at 37°C for 48 hours, and typical colonies on the plate were picked (milky white, round, with neat edges, raised, Opaque, wet and smooth surface), streak culture MRS solid medium, and isolate pure colonies. The purely cultured strains were inoculated into liquid MRS medium for culture, then added with 60% glycerol, and stored in a -80°C refrigerator. One of the Lactobacillus strains was named YL198.

[0040] 2. Identification of strains

[0041] Physiological and biochemical identification results of YL198: Gram staining positive, catalase test...

Embodiment 2

[0050] Embodiment 2 The preparation of wormwood Chinese medicine microecological fermentation preparation of the present invention

[0051] 1. Preparation of fermented bacteria suspension

[0052] Lactobacillus rhamnosus (Lactobacillus rhamnosus) YL198 (hereinafter referred to as YL198) was inoculated in liquid MRS medium, cultured statically at 37°C for 16 hours, centrifuged at 4°C and 6000rpm for 8 minutes, collected bacterial precipitates, and fermented and sterilized with aqueous medium solution Suspend, adjust the number of viable bacteria to 1.0×10 9 CFU / ml, to obtain YL198 bacterial suspension; wherein, the aqueous fermentation and sterilization medium solution was prepared by the following method: adding 30 g of corn steep liquor powder and 30 g of molasses to each liter of liquid medium, and autoclaving at 121 ° C for 15 minutes.

[0053] 2. Preparation of wormwood traditional Chinese medicine microecological fermentation preparation

[0054] The aboveground part of...

Embodiment 3

[0056] Embodiment 3 The influence of the wormwood traditional Chinese medicine microecological fermentation preparation of the present invention on the growth performance and serum biochemical indicators of weaned piglets

[0057] 1. For the experiment, 40 weaned piglets with similar age, weight and good health were selected and randomly divided into a control group and an experimental group. Each group had 2 replicates, and each replicate had 10 piglets. Starting from weaning at the age of 21 days, in addition to feeding with normal feed, the drinking water of the experimental group was added with Chinese herb wormwood microecological fermentation preparation (content: 3%), and the control group was only fed with normal feed and free drinking water, without adding Chinese herb wormwood Micro-ecological fermentation preparation, continuous feeding for 30 days.

[0058] 2. Growth performance index

[0059] The daily feed intake of piglets was recorded, and the body weight of e...

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Abstract

The invention discloses a wormwood traditional Chinese medicine micro-ecological fermentation preparation and a preparation method and application thereof, and relates to the field of livestock and poultry breeding. The wormwood traditional Chinese medicine micro-ecological fermentation preparation comprises lactobacillus rhamnosus YL198 bacterial suspension and wormwood, and the volume-to-weightratio of the lactobacillus rhamnosus YL198 bacterial suspension to the wormwood is 1L: 100kg-1L: 500 kg; the lactobacillus rhamnosus YL198 is preserved in China Center for Type Culture Collection on November 27, 2020, and the preservation number of the lactobacillus rhamnosus YL198 is CCTCC NO: M2020781. The lactobacillus rhamnosus YL198 is used for fermenting the traditional Chinese medicine wormwood; meanwhile, a magnetic field generated by terahertz ore is used in cooperation for assisting probiotic fermentation and fermentation conversion of wormwood bioactive components, the pH value of the obtained traditional Chinese medicine microecological preparation ranges from 3.0 to 3.5, and the product has the functions of enhancing body immunity, adjusting intestinal flora, improving the stomach and intestine and the like; the feed additive is green, environment-friendly, safe, effective and stable, and can be used for replacing or reducing antibiotics.

Description

technical field [0001] The invention relates to the technical field of livestock and poultry breeding, in particular to a microecological fermentation preparation of mugwort traditional Chinese medicine and its preparation method and application. Background technique [0002] Many food safety problems are caused by the detection of antibiotic residues in livestock and poultry products, which is caused by farmers not following national standards or strictly following drug withdrawal periods during the breeding process. The excessive use of antibiotics has always been a safety hazard in my country's breeding industry. It not only easily leads to antibiotic residues, but also causes endogenous infection and superinfection of animals after long-term use of antibiotics, reducing animal production performance and service life. Among them, endogenous infection means that antibiotics will cause microbial flora imbalance, reduce the beneficial flora in animals, and the original drug-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/282A61P37/04A61P1/00A61P29/00A23K10/30A23K10/12
CPCA61K36/282A61K2236/19A23K10/12A23K10/30A61P1/00A61P29/00A61P37/04
Inventor 李海权
Owner 上海植酵盛生物科技有限公司
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