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Chlamydomonas reinhardtii powder and application thereof in flour and rice food

A rhinestone and algae powder technology, which is applied in the field of food processing, can solve the problems of incomplete nutrition, unsatisfactory taste of staple food, and the inability of diabetics to eat normally, and achieve the effect of enhancing nutrition and immunity and high safety

Pending Publication Date: 2021-03-16
山西透云生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a Chlamydomonas reinhardtii powder and its application in flour and rice food, so as to solve the problems of incomplete nutrition, unsatisfactory taste of existing staple food and the inability of diabetics to eat normally.

Method used

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  • Chlamydomonas reinhardtii powder and application thereof in flour and rice food
  • Chlamydomonas reinhardtii powder and application thereof in flour and rice food
  • Chlamydomonas reinhardtii powder and application thereof in flour and rice food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Inoculate Chlamydomonas reinhardtii seeds to 6m 3 In the seed tank, the seed tank medium is: copper sulfate 80g, zinc oxalate 80g, sodium acetate 350g, potassium dihydrogen phosphate 200g, calcium hydroxide 800g, magnesium carbonate 10g, iron sulfate 20g, manganese sulfate 20g, ammonia water 0.3L, add water to 4m 3 , adjust the pH to 7.0 with acetic acid, then start the inoculation fermentation, the inoculum size is 0.5vol%. The culture temperature is 20°C, the pressure is 0.05 MPa, the dissolved oxygen is 50%, the stirring speed is 200 rpm, and the pH is maintained between 6.3 and 8.0 during the culture process. When a large amount of foam is produced in the tank, add a defoamer, the defoamer is 3 kg of corn oil and 1 kg of triglycerin, and the amount of defoamer added is 4 kg. During the fermentation process, after three days, 0.2 kg of medium was added every 2 hours. After 120 hours of fermentation, when the dry weight in the detected culture medium reaches 30 g / L...

Embodiment 2

[0033] 1. Hypoglycemic test in normal mice

[0034] 20 healthy female mice were selected and randomly divided into a control group and a high-dose group according to the blood glucose level measured after fasting for 5 hours. The control group was given distilled water, and the high-dose group was given the test sample (gavage with distilled water) at 3.0 g / kg BW. After continuous gavage for 30 days, the fasting blood glucose was measured for 5 hours, and the blood glucose values ​​of the two groups of mice were compared. The results are shown in Table 1.

[0035] Table 1: Effect of Chlamydomonas reinhardtii powder on fasting blood glucose in normal mice (mmol / L)

[0036] group Number of animals (only) Before the test After the test control group 10 7.22±0.76 6.99±0.81 high dose group 10 7.23±0.89 6.96±0.74

[0037] 2. Hypoglycemia test in hyperglycemia model mice

[0038] Using the hyperglycemia model of pancreatic islet damage: Randomly...

Embodiment 3

[0050] The first step: take the Chlamydomonas reinhardtii powder prepared in Example 1, add 60g of Chlamydomonas reinhardtii powder to 1kg wheat flour and mix evenly, set aside;

[0051] The second step: the water temperature is controlled at 30°C, according to the standard of 1kg wheat flour mixed with 0.3L water, water is added in proportion to the preparation of the first step, and the dough is fully mixed with a dough mixer for 10 minutes to make a dough;

[0052] Step 3: Put the dough into a static curing machine for 10 minutes. The prepared Chlamydomonas reinhardtii dough is used as a raw material, and other raw materials are added (according to a conventional method) for further deep processing to make noodles, pancakes, dumplings, steamed buns, steamed buns, deep-fried sticks and bread.

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Abstract

The invention provides chlamydomonas reinhardtii powder and application thereof in flour and rice food. A preparation method of the chlamydomonas reinhardtii powder comprises the following steps: (a)inoculating chlamydomonas reinhardtii seeds into a seed tank for heterotrophic culture, and after the culture, inoculating the chlamydomonas reinhardtii seeds with the inoculation quantity of 1-10vol%into a fermentation tank for heterotrophic fermentation; (b) after the fermentation is finished, carrying out membrane separation on the obtained fermentation liquid to obtain a chlamydomonas reinhardtii solid product; and (c) carrying out spray drying or vacuum freeze drying on the obtained product to obtain the chlamydomonas reinhardtii powder. The chlamydomonas reinhardtii powder disclosed bythe invention can be added when staple foods such as steamed buns, noodles, rice and rice noodles are made in daily life, has the effect of assisting in reducing blood sugar, and can enhance the nutrition and immunity of diabetic patients.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to Chlamydomonas reinhardtii powder and its application in flour and rice food. Background technique [0002] In recent years, with the continuous improvement of living standards, people's requirements for food quality have also gradually increased. In my country, rice noodles are the main staple food, and flour-based foods such as noodles, steamed buns, pancakes, etc., and rice-based foods such as rice noodles, etc., have a huge market share in my country. consumption base. [0003] There are many problems in the processing of existing flour and rice foods. Taking pasta as an example, the flour is prepared by peeling and crushing. It is usually made by adding water, stirring and mixing, and then steaming, boiling, and frying. Various forms of delicacies, but the refined flour is separated from the wheat cortex and germ during the refined process, resulting in a large loss o...

Claims

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Application Information

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IPC IPC(8): C12N1/12A23L7/10A23L33/10C12R1/89
CPCC12N1/12A23L7/10A23L33/10A23V2002/00A23V2200/324A23V2200/328
Inventor 牛志国王力平赵元芬周萍
Owner 山西透云生物科技有限公司
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