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Compound bacterial strain, fungicide and application of compound bacterial strain and fungicide

A technology of bacterial strains and bacterial agents, which is applied in the fields of compound bacterial strains, bacterial agents and their applications, and can solve problems such as high cost and large amount of sludge

Active Publication Date: 2021-03-16
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, the main purpose of the present invention is to propose a composite bacterial strain, bacterial agent and its application, the composite bacterial strain can directional transform nitrate and nitrite, realize efficient denitrification treatment, and can solve the problem of low C / N livestock and poultry The problems of high cost and large amount of sludge in the process of denitrification of biogas slurry

Method used

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  • Compound bacterial strain, fungicide and application of compound bacterial strain and fungicide
  • Compound bacterial strain, fungicide and application of compound bacterial strain and fungicide
  • Compound bacterial strain, fungicide and application of compound bacterial strain and fungicide

Examples

Experimental program
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Embodiment 1

[0035] This embodiment is the screening and purification of Enterobacteriaceae C1-3 bacterial strain and Pantoea A1 bacterial strain:

[0036] 1. Build a microbial strain library:

[0037] 1.1. Use SPIN Kit for Soil (MP Biomedicals, CA, USA) Soil DNA Extraction Kit is used to extract DNA from activated sludge samples that have been fully mixed and freeze-dried. The activated sludge samples are from Jinjing Institute of Subtropical Ecology, Chinese Academy of Sciences Activated sludge deposits in the livestock and poultry biogas slurry disposal device of the livestock and poultry breeding wastewater treatment system of the experimental station; and use F27 and 1492R as primers, and use the extracted DNA as a template to perform PCR amplification, and the 50ul amplification system is 2×Taq PCR Master Mix 25ul, F27 1ul, 1492R 1ul, DNA template 2ul, ddH2O2 1ul. The amplification program was pre-denaturation at 94°C for 1 min, denaturation at 94°C for 30 s, annealing at 57°C for ...

Embodiment 2

[0067]This embodiment is a bacterial agent, which contains Pantoea A1 strain and Enterobacter C1-3 strain, wherein the ratio of the number of Pantoea A1 strain to Enterobacter C1-3 strain is 1:1.

[0068] The preparation of the above bacterial agent is as follows:

[0069] Flush argon gas into the serum bottle to provide anaerobic conditions for anaerobic denitrifying microorganisms, inject Enterobacteriaceae C1-3 strains and Pantoea A1 strains at a ratio of 1:1 into 4ml sterile distilled water, and use a sterile syringe Inject into a serum bottle containing 40ml of LB liquid medium, and after culturing for 12 hours, collect the culture medium in the logarithmic growth phase, centrifuge it in a centrifuge at 5000rpm for 5 minutes, and then wash it 3 times with sterilized 1×PBS to remove residual LB medium, and then use 1×PBS to resuspend the bacteria to obtain the above bacterial preparation.

experiment example 1

[0071] This experimental example is the research on the biotransformation of nitrate by Enterobacteriaceae C1-3 strain

[0072] Flush argon gas into the serum bottle to provide anaerobic conditions for anaerobic denitrifying microorganisms, inject Enterobacter C1-3 strain into 4 ml sterile distilled water, and use a sterile syringe to inject serum containing 40 ml LB liquid medium In the bottle, after culturing for 12 hours, collect the culture medium in the logarithmic growth phase, and centrifuge it in a centrifuge at 5000rpm for 5 minutes, then wash it with sterilized 1×PBS three times to remove the residual LB medium, and then use 1×PBS Resuspended bacteria, OD of resuspended bacterial solution 600 1.42 and inoculated into sterile DM1 (KNO 3 As the only nitrogen source, the concentration is 30mg / L), and cultivated at 150rpm, 30°C for 72h, and each strain was set up in three parallels;

[0073] The results of nitrate conversion are as image 3 shown by image 3 It can b...

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Abstract

The invention discloses a compound bacterial strain, a fungicide and application of the compound bacterial strain and the fungicide. The compound bacterial strain consists of enterobacteriaceae C1-3 of which the collection number is CGMCC No.20581 and pantoea A1 of which the collection number is CGMCC No.20582, wherein the enterobacteriaceae C1-3 is obtained by separating and screening activated sludge. The invention also provides the application of the compound bacterial strain and the fungicide containing the compound bacterial strain in nitrogen removal treatment, and an efficient nitrogenremoval treatment effect is obtained. The compound bacterial strain nitrogen removal system constructed by the invention has a good application value on an aspect of nitrate (biogas liquid) nitrogen removal.

Description

technical field [0001] The invention relates to the technical field of denitrification, in particular to a compound bacterial strain, bacterial agent and application thereof. Background technique [0002] With the comprehensive control of point source pollution in urban areas, China's intensive livestock and poultry farming has gradually become prominent, and livestock and poultry breeding wastewater contains a large amount of chemical oxygen demand (COD) and ammonia nitrogen; in view of the characteristics of high organic matter in livestock and poultry wastewater, anaerobic Oxygen fermentation is used to obtain biogas with high value, so as to realize energy conversion. The anaerobic fermentation stage realizes the conversion of organic matter to CH 4 biotransformation, but the removal of ammonia nitrogen (ammonia nitrogen ≈ 500mg / L) is not obvious, so the COD / TN (total nitrogen) ratio of the biogas slurry is significantly reduced. At present, the COD / TN ratio of the biog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C02F101/16C12R1/01
CPCC12N1/20C02F3/34C02F2101/163Y02W10/10
Inventor 庄绪亮徐圣君徐立娜左佳靓张小寒姜参参杨东敏白志辉李瑞杨凡
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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