Collagen for preparing hydrogel and preparation method of collagen

A collagen and hydrogel technology, applied in the field of bioengineering, can solve the problems of poor adhesion and unsuitable for in vivo application, etc.
CN112521491AActive Publication Date: 2021-03-19JIANGNAN UNIV +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2021-03-19

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Abstract

The invention relates to collagen for preparing hydrogel and a preparation method of the collagen. A collagen sequence containing a bioactive structure GFPGER region is designed, cysteine with an oxidative crosslinking characteristic is introduced into a plurality of sites of the collagen sequence, and four kinds of cysteine-containing collagen with higher purity are obtained through heterologousexpression of escherichia coli. Hydrogen peroxide is used as a crosslinking agent for crosslinking the collagen to prepare the hydrogel. The prepared collagen has good thermosensitivity, can form stable hydrogel construction in an oxidation environment, has no toxicity to cells, and provides a good basis for the application of a drug controlled release carrier.
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Description

technical field

[0001] The invention relates to collagen for preparing hydrogel and a preparation method thereof, belonging to the technical field of bioengineering. Background technique

[0002] Collagen is an important class of biopolymer materials, the main component of animal connective tissue, and the most abundant and widely distributed functional protein in mammals, accounting for about 30% of the total protein. Collagen has good biocompatibility, low immunogenicity and tissue degradable absorbability. It can stop bleeding and heal wounds, fill, repair and reconstruct defective tissues, and diagnose, treat and repair organisms, as well as drug carriers and tissues. It is widely used in engineering and other aspects, and is an ideal medical biomaterial.

[0003] Natural collagen has a wide range of sources, mainly in animal tendons, bones, skin, ligaments and other tissues, and is the main source of collagen raw materials. The methods of extracting collagen include a...

Claims

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