Recombinant yeast for producing xanthophyll and application of recombinant yeast

Abstract

The invention discloses a recombinant yeast for producing xanthophyll. An octahydro-lycopene synthase gene, an octahydro-lycopene dehydrogenase gene, a lycopene epsilon cyclase gene, a lycopene beta cyclase gene, a carotene beta hydroxylase gene and a carotene epsilon hydroxylase gene are exogenously introduced into the yeast. The six enzymes capable of being successfully expressed in yeast are obtained through gene cloning and codon optimization, and a genetically engineered yeast strain for producing xanthophyll is constructed. The delta carotene is produced under the catalysis of phytoene synthase, phytoene dehydrogenase and lycopene epsilon cyclase, and then the delta carotene is converted into xanthophyll under the catalysis of lycopene beta cyclase, carotene beta hydroxylase and carotene epsilon hydroxylase.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a recombinant yeast for producing lutein and its application. Background technique

[0002] The chemical name of Lutein is 3,3'-diol-α-carotene, which can be synthesized in higher plants and algae, and plays an important role in physiological metabolism. As an important part of the plant photosystem, lutein captures light energy in photosynthesis, regulates plant growth and development, and participates in the interaction between plants and the environment as a biological signal molecule. Lutein is also an important component of retinal macular pigment, which has the function of absorbing blue light to protect the eyes. Since humans cannot produce lutein themselves, they need to be ingested from food. Studies have shown that lutein can prevent and treat retinal damage, including age-related macular degeneration, glaucoma, and diabetic retinopathy. As a nutraceutical, lutein can imp...

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