Method for preparing crystal of lycopene and concentrate

Abstract

The present invention relates to a preparation method of lycopene crystal and lycopene concentrate. Said method includes the following steps: pretreating raw material, using weakly alkaline solution to saponify raw material, then using low-molecular alcohol to make treatment, and using organic solvent to make extraction, finally two-temp. crystallizing so as to obtain the dark red lycopene crystal whose purity is greater than 70%, and the purity of lycopene crystal product produced by using said method can be up to above 90%. Said invention also provides its application range.

Description

technical field [0001] The invention relates to the field of food processing, in particular to a method for producing lycopene crystal products and lycopene concentrates by using a solvent to extract tomato peels separated from tomato paste, tomato powder or peel seeds. Background technique [0002] The molecular formula of Lycopene is C 40 h 56 , with a molecular weight of 536.85, and its structure is a polyunsaturated aliphatic hydrocarbon containing eleven conjugated double bonds and two non-conjugated double bonds. Strong coloring ability. Lycopene is one of the main sources of carotenoids in the diet and is also a biosynthetic intermediate of many carotenoids, accounting for more than 50% of carotenoids in serum. Its ability to quench singlet oxygen is 100 times that of vitamin E and more than twice that of β-carotene (Mascio D, Arch Biochem Biophys, 1989, 274:532-538). Epidemiological studies have found that the content of lycopene in the human ...

AI Technical Summary

Problems solved by technology

However, the huge equipment investment required by the supercritical fluid extraction technology and the higher operating costs limit the application in lycopene extraction, and the lycopene products obtained by using supercritical fluid extraction have low purity and low yield. not high

[0007] CN1298904A describes a method for producing lycopene with a purity of 10% from tomato paste, which uses water to stir, centrifugal dehydration, alkali washing, water washing, dry crushing, heating extraction, flash evaporation, crystallization, etc. to obtain lycopene crystals. The difference is that the product has low purity and the yield is only about 20%; CN1316468A discloses a method for separating and preparing high-purity lycopene from crude lycopene by high-speed countercurrent chromatography, and the product purity obtained by the method can reach 95% More than %, but using a variety of organic solvents, including highly toxic acetonitrile, chloroform, etc., while the production efficiency is low, the cost is high, the separation scale is limited, and it is not suitable for industrial production; IL107999 describes the production of lycopene oleoresin from tomato paste method, but did not obtain high-purity lycopene crystals; WO03 / 079816A1 announced a lycopene oleoresin with a lycopene content of 5-20% and a sugar content of less than 1%, and then purified to obtain a high-purity lycopene oleoresin In the method of 50% lycopene crystals, first wash and crush the tomato, heat and concentrate at 40-70°C, then extract with water-saturated ethyl acetate, wash the extract with water, and finally evaporate under reduced pressure to obtain lycopene oleoresin. Further wash the oleoresin with ethanol or ethyl acetate, filter and dry to obtain lycopene crystals with higher purity, the process is long and the operation is cumbersome; US5858700A discloses a method for separating and purifying lycopene from lycopene oleoresin by saponification reaction , but saponification makes the free lycopene fine crystals wrapped by a large amount of glyceride, which is difficult to separate; CN1472183A discloses a preparation method of high-purity lycopene, which uses lycopene oleoresin as raw material, at higher temperature and inert Under the protection of gas, it is dissolved in a hydrocarbon organic solvent to form a saturated solution, and the solution is filtered to remove impurities and then crystallized at a low temperature to finally obtain high-purity lycopene crystals. However, the disadvantage of this method is that the yield is low, and the lycopene The dissolution rate of oleoresin is slow, and the solution contains other immiscible impurity liquids, which can easily contaminate lycopene crystals

At present, many high-purity lycopene are purified from lycopene oleoresin. Usually, recrystallization is used and multiple washings are required. The process is long and the loss is increased.

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