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Pseudomonas chlororaphis and applications thereof

A technology of Pseudomonas chloropinus and bacterial strains, applied in Pseudomonas chloropinus and its application fields, can solve problems such as environmental pollution and residual drug damage, and achieve environmental friendliness and compatibility, strong inhibitory effect, and obvious selective inhibition The effect of action

Active Publication Date: 2021-04-02
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Chemical herbicides have made a significant contribution to my country's food production, but long-term repeated use of chemical herbicides has also brought negative effects such as environmental pollution, residual pesticide damage, and weed resistance.

Method used

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  • Pseudomonas chlororaphis and applications thereof
  • Pseudomonas chlororaphis and applications thereof
  • Pseudomonas chlororaphis and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Isolation of Pseudomonas chloropinus sj19

[0019] Take materials from the bamboo whip of a dragon scale bamboo, clean the newly dug bamboo whip, cut it into small pieces of about 2cm, first sterilize the surface with 70% alcohol, and then disinfect it with 50% antifomin (sodium hypochlorite solution) for half an hour , and finally soaked in sterile water and rinsed 4-5 times. Cut the above-mentioned treated materials on the ultra-clean bench and inoculate them on the PDA medium (i.e. solid potato medium) plate. When bacteria grow out, they are respectively streaked and purified, and the DNA is extracted for molecular identification, so that Obtain and confirm the strain. The specific steps for strain confirmation are as follows:

[0020] 1) Sample collection: Scrape a small amount of bacteria to be tested from the plate into a 200 μL PCR tube, add 100 μL of sterile water, centrifuge at high speed for a short time, and remove the supernatant;

[0021] 2) S...

Embodiment 2

[0027] Example 2: Morphological and biological characteristics of bacterial strains

[0028] The sj19 strain was initially isolated and cultured on PDA medium, and the colony showed a copper-colored metallic luster ( figure 1 a, b). Microscopic observation showed that the bacteria were short rod-shaped ( figure 1 .c). Further studies showed that the optimum temperature range of Pseudomonas chloropinus sj19 was 25-34°C, and it grew better on LB medium than on PDA medium, (eg figure 2 shown). In contrast, Sj19 grew slower on PDA medium than on LB medium (eg image 3 shown).

Embodiment 3

[0029] Embodiment 3: the bacteriostasis of bacterial strain

[0030] Pick the sj19 monoclonal clone in LB liquid medium, culture it at 28°C and 150rpm for 72h, then centrifuge at 4000rpm for 10min, filter the supernatant through a sterile filter membrane with a pore size of 0.22μm, take 0.2mL of the filtrate and spread it evenly on a PDA plate On the culture medium, an equal volume of sterile water was used as a control, and a 0.5 cm glial cell anthracis bacterial block ( Colletotrichum gloeosporioides ), Botrytis ( Botryosphaeria dothidea ) and Gibberella moniliforme ( Gibberella moniliformis ), and measure the growth diameter of mycelium after culturing at 28°C for 3 days. Each treatment was replicated 3 dishes. The mold colony diameter is measured by the cross method, and the mycelial growth inhibition rate is calculated, wherein the inhibition rate (%)=[(control mold colony diameter-treatment mold colony diameter) / (control mold colony diameter-bacteria block diameter...

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Abstract

The invention discloses pseudomonas chlororaphis and applications thereof, and belongs to the technical field of biology. The effects of the pseudomonas chlororaphis on controlling plant disease bacteria and inhibiting the growth of monocotyledon weed are also provided. The pseudomonas chlororaphis and secondary metabolite thereof are from nature and back to the nature, so that the pseudomonas chlororaphis is friendly to environment and compatible; the pseudomonas chlororaphis has good pertinence on inhibition of weeds of agricultural and forestry crops, and has the effects of inhibiting plantdiseases and insect pests while controlling the growth of the weeds; and the pseudomonas chlororaphis is pollution-free and residue-free, so that the pseudomonas chlororaphis can be used as a novel pollution-free microbial herbicide, which can be used for the harmless production of woodland herbicide.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a pseudomonas chloropin and its application. Background technique [0002] Chemical herbicides have made a significant contribution to my country's food production, but the long-term repeated use of chemical herbicides has also brought negative effects such as environmental pollution, residual phytotoxicity, and weed resistance. With the continuous improvement of human awareness of environmental protection, the call for reducing the use of chemical herbicides has become increasingly strong. These factors impel people to devote themselves to the development of new environmentally friendly biological herbicides; [0003] Microbial herbicides use weed pathogenic bacteria or their metabolites to control weeds. They process live weed pathogenic bacteria or their metabolites into a certain dosage form and use them to prevent and control weeds during the appropriate control per...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/27A01P3/00A01P13/02C12R1/38
CPCA01N63/27
Inventor 彭华正金群英朱汤军叶华琳张飞英
Owner ZHEJIANG FORESTRY ACAD
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