Special green alga polysaccharide lyase and production process thereof

A technology of chlorella polysaccharides and lytic enzymes, which is applied in the field of marine biology to achieve efficient degradation of Enteromorpha polysaccharides, high yield, and improved enzymatic hydrolysis effect

Pending Publication Date: 2021-04-02
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a polyanionic heterogeneous polysaccharide, Enteromorpha polysaccharide has a special sugar chain structure, and the existing tool enzymes cannot effectively hydrolyze it

Method used

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  • Special green alga polysaccharide lyase and production process thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A special green algae polysaccharide lyase, characterized in that the production process of the lyase specifically includes the following steps:

[0026] (1) Activation: inoculate microbial strains into the activation medium, adjust the initial pH to 7.0, and ferment at 28-35°C for 8 hours to obtain fermented seed liquid; the composition of the activation medium is: 2% glucose, Moss polysaccharide 1%, peptone 0.5%, sodium nitrate 0.5%, sodium chloride 1%, MgSO 4 0.10%, K 2 HPO 4 0.20%; the composition of the microbial strains is a mixed strain of Alteromonassp.A321, methylotrophic bacillus (Bacillus methylotrophicus) LJ, Sim bacillus (Bacillussiamensis) L13, and its compatibility ratio is 6:2:1;

[0027] (2) Fermentation broth preparation: prepare fermentation broth, adjust the initial pH to 7.0, and sterilize at 121°C for 30 minutes; the composition of the fermentation broth is: Enteromorpha 2%, peptone 0.5%, sodium nitrate 0.5%, chloride Sodium 1%, MgSO 4 0.10%...

Embodiment 2

[0032] A special green algae polysaccharide lyase, characterized in that the production process of the lyase specifically includes the following steps:

[0033] (1) Activation: inoculate microbial strains into the activation medium, adjust the initial pH to 7.0, and ferment at 28-35°C for 8 hours to obtain fermented seed liquid; the composition of the activation medium is: 2% glucose, Moss polysaccharide 1%, peptone 0.5%, sodium nitrate 0.5%, sodium chloride 1%, MgSO 4 0.10%, K 2 HPO 4 0.20%; The microbial strain is Alteromonassp.A321;

[0034] (2) Fermentation broth preparation: prepare fermentation broth, adjust the initial pH to 7.0, and sterilize at 121°C for 30 minutes; the composition of the fermentation broth is: Enteromorpha 2%, peptone 0.5%, sodium nitrate 0.5%, chloride Sodium 1%, MgSO 4 0.10%, K 2 HPO 4 0.20%;

[0035] (3) Inoculation: inoculate the fermented seed liquid into the fermentation liquid, the inoculum amount is 8%;

[0036] (4) Fermentation: ...

Embodiment 3

[0039] A special green algae polysaccharide lyase, characterized in that the production process of the lyase specifically includes the following steps:

[0040](1) Activation: Inoculate microbial strains into the activation medium, adjust the initial pH to 7.0, and ferment at 28-35°C for 8 hours to obtain fermented seed liquid; the composition of the activation medium is: 2% glucose, Moss polysaccharide 1%, peptone 0.5%, sodium nitrate 0.5%, sodium chloride 1%, MgSO 4 0.10%, K 2 HPO 4 0.20%; The microbial strain is methylotrophic bacillus (Bacillus methylotrophicus) LJ;

[0041] (2) Fermentation broth preparation: prepare fermentation broth, adjust the initial pH to 7.0, and sterilize at 121°C for 30 minutes; the composition of the fermentation broth is: Enteromorpha 2%, peptone 0.5%, sodium nitrate 0.5%, chloride Sodium 1%, MgSO 4 0.10%, K 2 HPO 4 0.20%;

[0042] (3) Inoculation: inoculate the fermented seed liquid into the fermentation liquid, the inoculum amount ...

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Abstract

The invention discloses special green alga polysaccharide lyase and a production process thereof, and belongs to the technical field of marine organisms. According to the invention, microorganisms such as Alteromonas sp.A321, Bacillus methylotrophicus LJ, Bacillus siamensis L13 and the like are utilized to prepare enteromorpha polysaccharide lyase through a fermentation method, and the lyase has the effects of efficiently degrading enteromorpha polysaccharide and remarkably reducing the viscosity and can provide an efficient enzyme preparation for extraction of enteromorpha active substances and preparation of oligosaccharides with different polymerization degrees in the future; the enteromorpha polysaccharide lyase is prepared through the fermentation method, the production process is simple, the yield of the enteromorpha polysaccharide lyase is high, the highest enzyme activity in a fermentation liquor can be 72.8 U / ml, and the lyase can provide strong support for industrialization development of enteromorpha polysaccharides and has relatively good industrialization application prospects.

Description

technical field [0001] The invention relates to a special-purpose green algae polysaccharide lyase and a production process thereof, belonging to the technical field of marine organisms. Background technique [0002] my country is rich in seaweed resources, and its resource utilization has become the focus of my country's marine economic development. Enteromorpha is a large-scale economical green algae, commonly known as moss strips and seaweed, belonging to the phylum Chlorophyta, Ulvaceae of the order Ulva. Research has found that Enteromorpha is rich in carbohydrates, protein, crude fiber, amino acids, fatty acids, polysaccharides, vitamins and various mineral elements, among which Enteromorpha polysaccharides have the highest content, mainly composed of rhamnose, xylose, glucose and a small amount of mannose It is composed of monosaccharides such as sugar, galactose, and arabinose, and contains uronic acid and sulfate groups. The main chain of polysaccharides contains a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88C12P19/00C12R1/07C12R1/01
CPCC12N9/88C12P19/00
Inventor 王海华庄松娟单俊伟
Owner 青岛海大生物集团股份有限公司
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