Preparation and application of Beauveria bassiana BB-7 metabolites

A technology of Beauveria bassiana and BB-7, applied in the field of microorganisms, can solve the problems of short-acting, strong toxicity, frequent use, etc.

Pending Publication Date: 2021-04-20
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the treatment of pet skin diseases is mainly antibiotic topical ointment or spray, which often has problems such as strong toxicity, frequent use, short-acting, and easy drug resistance during the treatment process

Method used

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  • Preparation and application of Beauveria bassiana BB-7 metabolites
  • Preparation and application of Beauveria bassiana BB-7 metabolites
  • Preparation and application of Beauveria bassiana BB-7 metabolites

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Screening of antifungal activity of metabolites of Beauveria bassiana

[0030] 1. Beauveria bassiana ( Beauveria bassiana ) BB-7 is an endophytic fungus with antifungal activity isolated from the root of Hosta japonica. Collect fresh and healthy roots of Hosta japonica, after surface disinfection of the root tissue of Hosta japonica, check the cleanliness of the ultra-clean workbench, check the rinse solution, and screen the aseptic tissue blocks by plant tissue imprinting method; cut the above-mentioned aseptic tissues on the aseptic workbench Form small pieces with a diameter of about 0.5 cm, place them in PDA medium, and culture them upside down in a constant temperature fungal incubator at 25 °C. When endophytic fungal mycelium grows outward along the tissue incision, use the tip mycelia picking method. Colonies with different shapes were picked, and after repeated purification, they were transferred to PDA slant medium for purification, numbering, and a...

Embodiment 2

[0043] Example 2: Screening of active antifungal polar parts of Beauveria bassiana fermentation broth

[0044] 1. Preparation of different solvent extraction parts of Beauveria bassiana fermentation broth: after freeze-drying Beauveria bassiana fermentation broth, disperse with appropriate amount of distilled water, put the dispersed sample in a separatory funnel, and use petroleum ether, chloroform, Ethyl acetate was extracted 1:1 (v / v), repeated 3 times, the extracts were combined, and the extracts were concentrated and dried under vacuum at 40°C to obtain petroleum ether, chloroform fractions, ethyl acetate fractions, and water fractions (lyophilized) .

[0045] 2. Determination of antifungal activity of different solvent extraction parts of Beauveria bassiana fermentation broth: dissolve the above four polar parts with 500 μL DMSO, dilute to 10 mg / mL with distilled water and add the melted PDA medium at 1:9 ( 40-50°C), so that the final concentration was 1 mg / mL, and the ...

Embodiment 3

[0052] Example 3: Preparation of Beauveria bassiana fermentation broth spray film agent and its antifungal animal pharmacodynamics evaluation

[0053] 1. Preparation of film-spraying agent: Select polyvinyl alcohol (PVA17-88) and polyvinylpyrrolidone (PVP-K30), which are commonly used in pharmacy, as film-forming materials, use water as solvent, and glycerin as humectant. Antioxidants and preservatives are added, and a certain amount of Beauveria bassiana fermented liquid freeze-dried powder is added, and after being screened by the preparation process, the film spraying agent is prepared.

[0054] 2. Animal drug efficacy test of spray film

[0055] 2.1 Preparation of test animals

[0056] Three days before the inoculation of the bacterial solution, the white rabbits were subcutaneously injected with dexamethasone sodium phosphate immunosuppressant at a dose of 2.5 mg / kg, once a day, and observed.

[0057] 2.2 Establishment of animal model of fungal infection

[0058] Rabbi...

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Abstract

The invention relates to an isolated culture method of a Beauveria bassiana BB-7 strain and an inhibition effect of metabolites of the Beauveria bassiana BB-7 strain on animal fungal skin diseases. The strain is preserved in China Center for Type Culture Collection at Wuhan University, Wuhan, China, on April 30, 2019, with the postcode of 430072 and the preservation number of CTCC NO: M2019260; and the strain has been subjected to whole genome sequencing. The fungal metabolites have good antibacterial activity on three animal pathogenic bacteria, i.e., Sabourauditeslanosus, Microsporumgypseum and Trichophyton mentagrophytes.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to the application of a metabolite of Beauveria bassiana in the preparation of antifungal drugs. Background technique [0002] In recent years, the prevalence of fungal pet skin diseases has increased year by year, which has become a distress and problem for the pet market. More importantly, the disease is a zoonotic disease that directly poses a serious threat to human health. However, at present, the treatment of pet skin diseases is mainly antibiotic topical ointment or spray, which often has problems such as strong toxicity, frequent use, short-acting, and easy drug resistance during the treatment process. The development of a new antifungal pet drug source with high efficiency, low toxicity and long-acting effect is imminent. [0003] In 2016, the Ministry of Agriculture clearly stated in the "National Veterinary Health Development Plan (2016-2020)" to support th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61K36/06A61P31/10A61P17/00C12R1/645
Inventor 梁晓霞陈楠谢昀宇吴强胡云锴
Owner SICHUAN AGRI UNIV
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