Preparation method and application of tobacco cembrane diterpene microemulsion

A kind of ceberane diterpene microemulsion, tobacco technology, applied in the directions of botanical equipment and method, application, biocide and the like

Active Publication Date: 2021-04-27
TOBACCO RES INST CHIN AGRI SCI ACAD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the research and application of microemulsions prepared as fungicides using tobacco cembradane diterpene extracts as raw materials

Method used

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  • Preparation method and application of tobacco cembrane diterpene microemulsion
  • Preparation method and application of tobacco cembrane diterpene microemulsion
  • Preparation method and application of tobacco cembrane diterpene microemulsion

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Experimental program
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Effect test

Embodiment 1

[0022] Bacteriostasis experiment of embodiment 1 tobacco cembradane diterpenes

[0023] Refer to the ZL 201711082390.1 and ZL 201510382060.9 methods, use fireworks, non-applicable grade tobacco leaves or tobacco branches as raw materials, use 80-100% ethanol as the extraction solvent, and the amount of solvent is 1.2-1.5 times the volume of the extraction raw materials, and shake the extraction at room temperature and pressure Or continuous flow extraction, the extraction time is 2min-3min, continuous extraction 2-3 times, the extract is concentrated under reduced pressure, the concentrated solution is left standing at 2°C-8°C, flocculent precipitation appears in the concentrated solution, add activated carbon, stir Adsorbed for 5 minutes, filtered and concentrated to obtain tobacco cembradane diterpene extract extract.

[0024] (1) Determination of the antibacterial rate of cembrane diterpene to different pathogenic fungi

[0025] Tobacco cembradane diterpene extract extract...

Embodiment 2

[0035] Embodiment 2 prepares tobacco cembradane diterpene microemulsion

[0036]Mix the extract of tobacco cembradane diterpene, ethanol, fatty alcohol polyoxyethylene ether, and n-butanol in the ratio described in Table 1, stir and dissolve at a speed of 60-80r / min, and continue to stir for 30min; Sodium benzenesulfonate is added into water, stirred and dissolved; under stirring condition, the oil phase is slowly added into the water phase, after the mixing is completed, the stirring is continued at 60-80r / min for 30min, and the tobacco cembradane diterpene microemulsion is obtained.

[0037] Table 2 Tobacco cembradane diterpene microemulsion formulation

[0038]

[0039] The quality parameter that obtains tobacco cembradane diterpene microemulsion is as follows:

[0040] Table 3 8% Tobacco Cembradane Diterpene Microemulsion Quality Specifications

[0041] Quality technical indicators measured value Detection method Transparent temperature range -4℃-60...

Embodiment 3

[0042] The bacteriostasis activity of embodiment 3 cembradane diterpene microemulsions

[0043] Strawberry gray mold (Botrytis cinerea), apple rot fungus (Valsa mali Miyabeet Yamada), tobacco black shank fungus (Phytophthora parasitica var. Rate method, respectively measure the activity of tobacco cembradiene diterpenes and its microemulsion preparations to the tested strains, establish the toxicity method of tobacco cembratriene diol concentration and its logarithm of mycelial growth inhibition rate, according to the toxicity equation, calculate Cembratrienediol is to the concentration (EC 50 ).

[0044] According to the test results, the EC of tobacco cembratrienediol inhibiting the mycelial growth of strawberry botrytis cinerea, apple rot fungus and tobacco black shank fungus 50 The values ​​are: 10.75, 10.64, 23.77, after preparing the tobacco cembradane diterpene extract into microemulsion, its EC 50 The values ​​are greatly reduced, respectively: 3.94, 2.74, and 5.4. ...

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Abstract

The invention belongs to the field of plant disease control. The invention provides a preparation method and application of a tobacco cembrane diterpenoid bactericidal microemulsion in order to solve the technical problems that the prevention and treatment effect of tobacco cembrane diterpenoid in the development process of botanical pesticides is unclear, and a green dosage form formula with commercial value is not formed. The tobacco cembrane diterpenoid bactericidal microemulsion is prepared from the following components in percentage by weight: 6-8% of cembratriene diterpene extract, 15-20% of ethanol, 15-20% of fatty alcohol-polyoxyethylene ether, 5-8% of sodium dodecyl benzene sulfonate, 5-8% of n-butyl alcohol and the balance of water. The microemulsion has a better or equivalent control effect on apple canker, crop gray mold, black shank, root rot, powdery mildew and the like than or equal to a control chemical pesticide, does not contain any toxic organic solvent, and is good in pesticide effect, low in production cost and safe to crops and the environment.

Description

technical field [0001] The invention belongs to the field of plant disease prevention and control, and in particular relates to a preparation method and application of tobacco cembradane diterpene microemulsion. Background technique [0002] Cembrane diterpene belongs to the fourteen-membered macrocyclic diterpenes, which is a kind of natural product with fourteen-membered rings, three symmetrically distributed methyl groups and one isopropyl group in the parent skeleton. It mainly exists in tobacco, turpentine, frankincense and marine In organisms (mainly soft corals and gorgonians), it has various activities such as antibacterial, antitumor, and neuroprotective. There are many types of cembradane diterpenoids in tobacco, and 125 kinds have been reported so far. Among them, cembratriene diol is the most abundant cembradane diterpene in tobacco, accounting for more than 70% of the total amount of tobacco. FERCHMIN et al. (2013) conducted acute toxicity and subchronic toxici...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A01N25/04A01N31/06A01P3/00
CPCA01N25/04A01N31/06
Inventor肖勇杜咏梅刘雨露余祥文刘艳华张建会张鹏杨兴有侯小东
OwnerTOBACCO RES INST CHIN AGRI SCI ACAD