Serum-free neural precursor cell culture medium as well as preparation method and application thereof

Abstract

The invention provides a serum-free neural precursor cell culture medium as well as a preparation method and application thereof. The serum-free neural precursor cell culture medium comprises a DMEM / F12 culture medium, non-essential amino acids, human basic fibroblast growth factors, heparin, insulin, total iron transferrin, progesterone and putrescine. When the serum-free neural precursor cell culture medium is used for culturing mesenchymal stem cells (MSCs), the efficiency of differentiating the MSCs into neural precursor cells can be improved, and the yield of the neural precursor cells is increased.

Description

technical field [0001] The invention relates to the technical field of cell biology, in particular to a serum-free culture medium for neural precursor cells and its preparation method and application. Background technique [0002] Injury or dysplasia of the central nervous system can lead to apoptosis of nerve cells, defects of nerve tissue, defects in motor and thinking abilities, and eventually lead to permanent disability or even shortened life span. The self-repair ability of the central nervous system is extremely low, and the treatment of such diseases requires the use of cell replacement therapy to supplement the apoptotic and defective nerve cells and tissues, thereby restoring the function of the nervous system. [0003] Neural precursor cells (Neural precursor cells, NPCs) play an important role in the development of the nervous system and the repair of nerve damage. The difficulty in repairing adult nerve damage is related to the lack or silence of NPCs. Previous...

AI Technical Summary

Problems solved by technology

However, although the medium with chemical inducers can be used to rapidly induce the generation of neural-like cells, the cell viability is poor, and death or apoptosis occurs in an average of 4 days.

The use of the medium added with cell growth factors has defects such as low induction efficiency, long induction period, and short survival time. Some literatures even believe that the nerve-like cells generated by the neural factor induction method are only morphologically like nerve cells, but do not have nerve cells. The function of the cells; the above-mentioned defects cannot be avoided by using the medium supplemented with chemical inducers and growth factors at the same time

The use of culture medium added with various cells and brain tissue fluids has the disadvantages of cell collection complex or heterologous heterogeneous proteins, resulting in immune rejection and disease transmission

[0005] In summary, there are some disadvantages in the use of the above-mentioned media, including low efficiency, long time-consuming, poor system repeatability or poor activity after cell induction, resulting cells are not pure, etc., and finally functional cells cannot be obtained.

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