Aflatoxin B1 detection method based on up-conversion and black phosphorus nanosheet aptamer sensor

A technique for the detection of aflatoxin and aflatoxin B1, which is applied in the field of detection of aflatoxin B1, which can solve the problems of being unable to meet the requirements of rapid and high-sensitivity detection of AFB1 content, low detection accuracy and sensitivity of AFB1, and the inability to achieve specific detection of AFB1, etc. Achieve the effects of eliminating background fluorescence and interference from other molecules, good practical prospects, and ensuring food security

Active Publication Date: 2021-04-30
JIANGSU UNIV
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  • Description
  • Claims
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Problems solved by technology

However, traditional aflatoxin detection methods, such as high performance liquid chromatography, enzyme-linked immunosorbent assay, etc., are not only expensive in equipment, high in detection costs, and cumbersome in steps, but more importantly, they cannot achieve specific detection of AFB1. Therefore, the requirements for rapid and highly sensitive detection of AFB1 content cannot be met, and the detection accuracy and sensitivity of AFB1 are at a low level

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  • Aflatoxin B1 detection method based on up-conversion and black phosphorus nanosheet aptamer sensor
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  • Aflatoxin B1 detection method based on up-conversion and black phosphorus nanosheet aptamer sensor

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Embodiment 1

[0046] The detection of aflatoxin B1 content in the peanut oil of embodiment 1

[0047] A kind of aflatoxin B1 detection method based on the aptamer sensor of up-conversion and black phosphorus nanosheet, comprises the steps:

[0048] Step 1, preparation of amino-functionalized up-conversion nanomaterials: Accurately weigh 0.2366g of yttrium chloride hexahydrate, 0.0775g of ytterbium chloride hexahydrate, 0.0076g of erbium chloride hexahydrate, 0.1461g of sodium chloride and 0.1852g of fluoride Ammonium was added to 15mL ethylene glycol solution, ultrasonically dissolved for 10min, transferred to a 100mL round bottom flask with magnetic stirring for 30min, and mixed evenly; under dark conditions, 0.2g polyethyleneimine was added to the mixed solution and fully stirred for 10min; The mixed solution was transferred to a polytetrafluoroethylene-lined autoclave, and reacted at 200°C for 12 hours to obtain the reaction product; after the reaction product was cooled, it was precipit...

Embodiment 2

[0062] The detection of aflatoxin B1 content in the wheat of embodiment 2

[0063] A kind of aflatoxin B1 detection method based on the aptamer sensor of up-conversion and black phosphorus nanosheet, comprises the steps:

[0064] Step 1, preparation of amino-functionalized up-conversion nanomaterials: Accurately weigh 0.2366g of yttrium chloride hexahydrate, 0.0775g of ytterbium chloride hexahydrate, 0.0076g of erbium chloride hexahydrate, 0.1461g of sodium chloride and 0.1852g of fluoride Ammonium was added to 15mL ethylene glycol solution, ultrasonically dissolved for 15min, transferred to a 100mL round bottom flask with magnetic stirring for 40min, and mixed evenly; under dark conditions, 0.2g polyethyleneimine was added to the mixed solution and fully stirred for 10min; Transfer the uniformly mixed solution to a polytetrafluoroethylene-lined autoclave, and react at 180°C for 8 hours to obtain the reaction product; after the reaction product is cooled, precipitate it with 2...

Embodiment 3

[0072] The detection of aflatoxin B1 content in the embodiment 3 peanuts

[0073] A kind of aflatoxin B1 detection method based on the aptamer sensor of up-conversion and black phosphorus nanosheet, comprises the steps:

[0074] Step 1, preparation of amino functionalized up-conversion nanomaterials: Accurately weigh 0.2366g yttrium chloride hexahydrate, 0.0775g ytterbium chloride hexahydrate, 0.0076g erbium chloride hexahydrate, 0.1461g sodium chloride and 0.1852g Ammonium fluoride was added to 15mL ethylene glycol solution, ultrasonically dissolved for 15min, transferred to a 100mL round bottom flask with magnetic stirring for 40min, and mixed evenly; under the condition of avoiding light, 0.2g polyethyleneimine was added to the mixed solution and fully stirred for 10min ; Transfer the uniformly mixed solution to a polytetrafluoroethylene-lined autoclave, and react at 190°C for 12 hours to obtain the reaction product; Ethanol was centrifuged and washed 3 times; the obtained...

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Abstract

The invention discloses an aflatoxin B1 detection method based on an up-conversion and black phosphorus nanosheet aptamer sensor, and relates to the field of food safety detection. The detection method comprises the following steps: step 1, preparing an amino-functionalized up-conversion nano material; 2, connecting an aflatoxin B1 aptamer to the surface of the amino-functionalized up-conversion nano material; 3, preparing a black phosphorus nanosheet; 4, dissolving the up-conversion nanoparticles with the aptamer connected to the surfaces obtained in the step 2 in a buffer solution, adding a dispersion solution prepared from the black phosphorus nanosheets obtained in the step 3, and carrying out incubating and combining to obtain a specific detection system; 5, establishing an aflatoxin B1 content detection standard curve; and 6, detecting the content of aflatoxin B1 in the sample. By constructing an aflatoxin B1 fluorescence detection system, high specificity and sensitivity detection of aflatoxin B1 in food is achieve, and the method has a wide concentration detection range and a low detection limit and has a good practical prospect.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to an aflatoxin B1 detection method based on an aptamer sensor of upconversion and black phosphorus nanosheets. Background technique [0002] Aflatoxin is a secondary metabolite produced by Aspergillus flavus and Aspergillus parasiticus, and is a highly toxic and carcinogenic mycotoxin. Aflatoxins mainly include group B (B1 and B2), group G (G1 and G2) and group M (M1 and M2), among which aflatoxin B1 (AFB1) is the most toxic and is listed as Class I carcinogens. [0003] AFB1 can lead to a series of health hazards such as severe liver damage, liver cancer, and growth disorders in children. Unfortunately, according to the report of the Food and Agriculture Organization of the United Nations, about 25% of the world's crops are damaged by mycotoxins, and AFB1 pollution alone accounts for a quarter of the total crops damaged by mycotoxins. [0004] Due to the serious h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 吴继忠陈全胜欧阳琴荣雅文李欢欢王丽徐夏霏
Owner JIANGSU UNIV
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