Biochemical analysis method for simultaneously detecting multiple target objects

A technology for biochemical analysis and target objects, applied in the field of biochemical analysis, can solve problems such as cumulative errors, lower analysis efficiency, and inability to realize simultaneous detection of multiple targets, and achieve the effects of fast speed, improved analysis efficiency, and fast recognition speed

Active Publication Date: 2021-05-28
HUAZHONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method enables ultrasensitive, absolute quantification of disease-associated proteins, but cannot simultaneously detect multiple targets
In addition, this method requires multiple magnetic separations and the addit

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  • Biochemical analysis method for simultaneously detecting multiple target objects
  • Biochemical analysis method for simultaneously detecting multiple target objects
  • Biochemical analysis method for simultaneously detecting multiple target objects

Examples

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Example Embodiment

[0064]Example 1 Detection of a variety of inflammatory markers in blood using a capillary separation method

[0065]Four inflammatory markers in blood samples were used in a blood sample, a multivariate detection of four inflammatory markers in blood samples, calcitonin (PCT), C-reactive protein (CRP), serum amyloid (SAA), Its principleFigure 4 As shown: The surface is modified with the captured antibody corresponding to IL-6, PCT, CRP, SAA, and 1 μm red PS microspheres, 1 μm blue PS microspheres, 1 μm red PS microspheres, 3 μm red PS microspheres Microspheres, 3 μm Blue PS microspheres are signal probes, and the detection antibodies corresponding to IL-6, PCT, CRP, SAA, respectively. When the target is present, the capture antibody, the target, the target, and the detection resistance of the signal probe surface form a sandwich composite, resulting in a PS microsphere and different particle size, color PS of 50 μm. The microspheres are coupling, causing the free PS microsphere signal ...

Example Embodiment

[0092]Example 2 Multi-edged detection of Salmonella, mono-Listeria, gold-yellow Staphylococcus, using a composite membrane separation method

[0093]DNA hybrid reactions against Salmonella, plum in Listeria, Golden Colored Staphylococcus,Figure 5 As shown: The surface of the Salmonella, single-Listeria, and S. S. Smectica, S. microspaths, respectively, of the PS microspheres of 50 μm. The detection probe coupling of Salmonella, single Ristercius, S. aureus is added. When the target is present, the capture probe is modified on the surface of the carrier, the target, and the detection probe modified in the surface of the signal probe will form a complex by DNA hybridization, resulting in a PS microsphere and different grains having a particle size of 50 μm. The PS microspheres coupling of the diameter, causing the free PS microsphere signal probe in the solution to decrease. When there is no target, since the formation of a DNA hybrid reaction complex is not formed, the PS microspheres h...

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Abstract

The invention discloses a biochemical analysis method for simultaneously detecting multiple target objects, which combines biochemical reaction with visual microsphere counting, machine vision and deep learning, and respectively couples biological recognition molecules corresponding to the target objects to be detected on the surfaces of a carrier and a signal probe to perform immunoreaction or DNA molecular hybridization reaction. The method comprises the following steps: firstly, detecting a carrier and a signal probe, then separating the carrier and the signal probe by using a capillary tube or a composite film according to different particle sizes of the carrier and the signal probe, and finally, directly carrying out optical microscopic imaging counting on the separated signal probe. According to the invention, the signal probes with different particle sizes or colors are used for distinguishing different detection objects, so that simultaneous detection of multiple target objects is finally realized.

Description

technical field [0001] The invention belongs to the field of biochemical analysis and relates to a biochemical analysis method for simultaneously detecting multiple targets. Background technique [0002] With the development of the country and the improvement of people's living standards, the challenges faced in the fields of food safety, in vitro diagnostics and environmental monitoring are increasing day by day. Efficient detection methods have an extremely important impact on ensuring food safety, promoting human health, and ecological environment safety. . Most of the traditional detection methods rely on large-scale precision instruments and equipment. Although they have the characteristics of high precision and good accuracy, most of the instruments are expensive and inconvenient to carry. Before use, complex pre-treatment of the test samples is required. There are also stricter requirements for personnel. [0003] Immunoassay methods based on antibody-antigen recogn...

Claims

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Application Information

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IPC IPC(8): C12Q1/6834G01N33/546G01N33/566
CPCC12Q1/6834G01N33/54313G01N33/566C12Q2523/301C12Q2563/149C12Q2523/303
Inventor 陈翊平何慧禹赵维琦聂荣彬周阳
Owner HUAZHONG AGRICULTURAL UNIVERSITY
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