Forming mode of nano-silver coating on stem cell culture dish under assistance of magnetron sputtering and chemical gradient sedimentation

Abstract

The invention discloses a forming mode of a nano-silver coating on a stem cell culture dish under assistance of magnetron sputtering and chemical gradient sedimentation. The forming mode comprises the following steps: a, disinfecting the stem cell culture dish, and carrying out high-frequency oscillation cleaning; b, placing the stem cell culture dish in a steady state, removing a three-dimensional maximum diameter under a horizontal condition, customizing a silver target, and placing the stem cell culture dish into the silver target; c, bombarding the target object silver target through electromagnetic sputtering, winding 860 copper wire turns with the diameter of 0.1 cm on an outer target on the outer side of a magnetron sputtering vacuum chamber, and cleaning the vacuum chamber; placing a substrate material (the stem cell culture dish) in an inner target, sealing the vacuum chamber, and starting to vacuumize the vacuum chamber with the vacuum degree of 10 <-6 >; and d, inputting inert gas into the vacuum chamber for filling. The stem cell culture dish formed by the mode has the effect of killing bacteria, fungi, mycoplasma, chlamydia, spirochetes and even trichomonad in clinical use, and does not generate drug resistance.

Description

technical field [0001] The invention relates to the technical fields of biomedicine, biomedicine, nanotechnology and chemical gradient sedimentation, in particular to a method for forming a nano-silver coating on a stem cell culture dish assisted by magnetron sputtering and chemical gradient sedimentation. Background technique [0002] Nanometer (nm) is the smallest unit of measurement after the micron in the physics world, with the development of nanotechnology. A large number of nanomaterials have been found to have antiphysical phenomena. As well as the quality and effect of special functions, nano-silver is also after the extensive development of metal nano-materials. an emerging field of research. Silver in the nanometer state has produced a qualitative leap in the bactericidal ability used in biomedicine. A very small amount of nano-silver can produce a powerful bactericidal effect that was unimaginable in the past. Nano-silver can kill more than 650 kinds of bacter...

AI Technical Summary

Problems solved by technology

[0003] The stem cell culture dish in the prior art is an ordinary polymer material, which cannot effectively protect the stem cells from being eroded by external microorganisms, especially the erosive growth of bacteria during the process of culturing stem cells.

[0004] Moreover, ordinary polymer stem cell culture dishes cannot improve the microcirculation of the tissue around the wound, and at the same time cannot effectively promote wound healing and promote the repair and regeneration of damaged cells, and cannot eliminate the inflammatory response

Can not effectively activate and promote the growth of damaged tissue cells, reduce scar formation, and accelerate wound healing