Raman sensing analysis method for detecting enterotoxin

An analysis method, enterotoxin technology, applied in the field of optical analysis, to achieve the effect of enhancing SERS signal, realizing detection and improving sensitivity

Active Publication Date: 2021-05-28
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Raman sensing analysis method for detecting enterotoxin
  • Raman sensing analysis method for detecting enterotoxin
  • Raman sensing analysis method for detecting enterotoxin

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[0030] Example 1

[0031] 1. Preparation of plasmonic nanomaterial Au-Ag Janus NPs:

[0032] ① Preparation of monodisperse Au NPs:

[0033] Au NPs were prepared as seeds by citric acid reduction method. 0.8mL HAuCl 4 Mix with ultrapure water and heat to boiling. Quickly add 0.9mL sodium citrate solution under vigorous stirring, and the mixed solution continues to boil for 15min. Then cool down to room temperature naturally, and centrifuge and wash with ultrapure water to obtain Au NPs;

[0034] ② Preparation of Au-Ag Janus NPs:

[0035] Under vigorous stirring, 20 μL of 0.8 mM 2-mercaptobenzimidazole-5-carboxylic acid (MBIA) was added to 1 mL of citric acid-stabilized Au NPs synthesized in step (1). The mixed solution was incubated in a water bath at 60°C for 2h. After the reaction, the solution was cooled to room temperature, and 50 μL of 8 mM hydroquinone (HQ) and AgNO at a final concentration of 50 μM were added at a constant speed under vigorous stirring. 3 solutio...

Example Embodiment

[0045] Example 2

[0046] 1. Preparation of plasmonic nanomaterial Au-Ag Janus NPs:

[0047] ① Preparation of monodisperse Au NPs:

[0048] Au NPs were prepared as seeds by citric acid reduction method. 1.0mL HAuCl 4 Mix with ultrapure water and heat to boiling. Quickly add 1.0mL sodium citrate solution under vigorous stirring, and the mixed solution continues to boil for 17.5min. Then cool down to room temperature naturally, and centrifuge and wash with ultrapure water to obtain Au NPs;

[0049] ② Preparation of Au-Ag Janus NPs:

[0050] Under vigorous stirring, 22 μL of 1 mM 2-mercaptobenzimidazole-5-carboxylic acid (MBIA) was added to 1.1 mL of citric acid-stabilized Au NPs synthesized in step (1). The mixed solution was incubated in a water bath at 65°C for 2.5h. After the reaction, the solution was cooled to room temperature, and 60 μL of 10 mM hydroquinone (HQ) and AgNO with a final concentration of 52.5 μM were added at a constant speed under vigorous stirring. ...

Example Embodiment

[0060] Example 3

[0061] 1. Preparation of plasmonic nanomaterial Au-Ag Janus NPs:

[0062] ① Preparation of monodisperse Au NPs:

[0063] Au NPs were prepared as seeds by citric acid reduction method. 1.2mL HAuCl 4 Mix with ultrapure water and heat to boiling. Quickly add 1.1mL sodium citrate solution under vigorous stirring, and the mixed solution continues to boil for 20min. Then cool down to room temperature naturally, and centrifuge and wash with ultrapure water to obtain Au NPs;

[0064] ② Preparation of Au-Ag Janus NPs:

[0065] Under vigorous stirring, 24 μL of 1.2 mM 2-mercaptobenzimidazole-5-carboxylic acid (MBIA) was added to 1.2 mL of citric acid-stabilized Au NPs synthesized in step (1). The mixed solution was incubated in a water bath at 70°C for 3h. After the reaction, the solution was cooled to room temperature, and 70 μL of 12 mM hydroquinone (HQ) and AgNO with a final concentration of 55 μM were added at a constant speed under vigorous stirring. 3 so...

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Abstract

The invention provides a Raman sensing analysis method for detecting enterotoxin, and belongs to the technical field of light analysis. The method comprises the following steps: doping cesium bromide (CsBr) powder and lead bromide (PbBr2) powder into mesoporous silica (MSNs), and preparing space-hindered CsPbBr3-coated MSNs through a high-temperature solvent removal method; growing an Ag island on an Au core through a ligand 2-mercaptobenzimidazole-5-carboxylic acid (MBIA), and synthesizing Au-Ag Janus NPs. In addition, the ligand MBIA acts as a Raman beacon molecule, and shows a very unique and stable Raman signal. The composite material formed by the Au-Ag Janus NPs and the CsPbBr3 (at) MSNs through an antigen and an antibody can be used for greatly amplifying an SERS signal. The Raman signal of MBIA is used as a detection signal so that additional modification of a Raman beacon is avoided. The detection method is high in sensitivity and strong in specificity.

Description

technical field [0001] The invention belongs to the technical field of optical analysis, in particular to a Raman sensing analysis method for detecting enterotoxin. Background technique [0002] Staphylococcus aureus enterotoxins (SEs, including SEA, SEB, SEC, SED, and SEE) are considered to be common toxins that cause food poisoning, causing bacterial food poisoning, bacteremia, skin tissue infection, and other diseases. It is not allowed to be detected in food. As the most common Staphylococcus aureus enterotoxins in dairy products and the environment, sensitive and accurate detection of SEs is of great significance for food safety. At present, the methods for detecting enterotoxin mainly include: enzyme-linked immunoassay, mass spectrometry, PCR, electrochemical immunoassay, etc., but there are disadvantages such as high detection limit, cumbersome operation, expensive reagent equipment, time-consuming, and low sensitivity. Food safety poses an urgent requirement for ac...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N33/543G01N33/68
CPCG01N21/658G01N33/68G01N33/54346G01N2333/31
Inventor 赵媛施丽霞
Owner JIANGNAN UNIV
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