Age-related complementary food nutrition bag rich in hypoallergenic heterologous immunocompetence peptides and preparation method thereof

Abstract

The invention relates to an age-related complementary food nutrition bag rich in hypoallergenic heterologous immunocompetence peptides and a preparation method thereof, and belongs to the technical field of age-related nutrition supplement food. Twelve nutrient supplement factors are formed on three low-sensitization protein-based carriers, namely sea cucumber protein peptide powder, terrestrial plant source-soybean protein peptide powder and terrestrial animal source high-quality protein-skimmed milk powder for the first time; I-level steady-state protection of nutrient supplementation factors, II-level steady-state protection formed on a low-sensitization protein-based carrier and a III-level steady-state protection technology of double quantitative precise filling are designed, and the senile complementary food nutrition bag rich in the low-sensitization heterologous immunocompetence peptide is created; and the symbolic characteristics of low sensitization, high immunocompetence and high stability of the compound are confirmed through inspection of three indexes including a BALB / C mouse sensitization evaluation model, a mouse macrophage RAW264.7 cell model and the decay rate of vitamin A acetate, cholecalciferol and cyanocobalamin, and technical support is provided for development of series products.

Description

technical field

[0001] The invention belongs to the technical field of supplementary nutritional supplements for the elderly, and relates to a kind of 12 kinds of nutrient supplement factors that are stably formulated into marine source-sea cucumber immunoactive peptide powder, terrestrial plant source-soybean immunoactive peptide powder, terrestrial animal Source high-quality protein-skimmed milk powder 3 kinds of low-allergenic protein peptide-based carriers, and create a quality control technology for elderly supplementary food nutrition packages rich in low-allergenic heterologous immune active peptides. Background technique

[0002] "Nutrition package" is a kind of supplementary nutritional supplement made of soybeans, dairy products, etc. as food base materials, adding a variety of micronutrients and other auxiliary materials. According to the nutritional needs of different groups of people, it is usually divided into supplementary food nutrition packages for pregnant ...

Examples

Embodiment 1

[0040] (1) Preparation of hypoallergenic sea cucumber immunoactive peptide powder: the sea cucumber dry powder with protein content ≥ 30% is prepared with deionized water as the initial solution of sea cucumber protein with a protein concentration of 8%. After 15 minutes of homogenizer slurry treatment, remove the air bubbles on the surface of the sea cucumber protein slurry and pour it into the high-voltage pulse electric field feeding tank. Set the high-voltage pulse electric field strength to 15kV / cm, the pulse frequency to 2000 Hz, and the pulse width to 50μs, the flow rate is set to 2.5L / h treatment conditions, turn on the high-voltage pulse electric field to treat all the sea cucumber protein slurry for 6 cycles, and then prepare the denatured slurry liquid of sea cucumber protein, then introduce it into the enzymatic hydrolysis tank, and heat it while stirring. Heat up to 50°C, adjust the pH value to 9.5, add alkaline protease according to 2% of the added amount of sea c...

Embodiment 2

[0044] (1) Preparation of hypoallergenic sea cucumber immunoactive peptide powder: the sea cucumber dry powder with protein content ≥ 30% is prepared with deionized water as the initial solution of sea cucumber protein with a protein concentration of 5%. After 7.5 minutes of homogenizer slurry treatment, remove the air bubbles on the surface of the sea cucumber protein slurry and pour it into the high-voltage pulse electric field feeding tank. Set the high-voltage pulse electric field strength to 5kV / cm, the pulse frequency to 1000 Hz, and the pulse width to 20μs, the flow rate is set at 1.0L / h treatment conditions, turn on the high-voltage pulse electric field to process all the sea cucumber protein slurry for 2 cycles, and then prepare the denatured slurry liquid of sea cucumber protein, then introduce it into the enzymatic hydrolysis tank, and heat it while stirring. The temperature is raised to 52°C, the pH value is adjusted to 10.0, and alkaline protease is added according...

Embodiment 3

[0048] (1) Preparation of hypoallergenic sea cucumber immunoactive peptide powder: the sea cucumber dry powder with protein content ≥ 30% is prepared with deionized water as the initial solution of sea cucumber protein with a protein concentration of 7%. After the homogenizer is mixed for 5 minutes, remove the air bubbles on the surface of the sea cucumber protein slurry and pour it into the high-voltage pulse electric field feeding tank. Set the high-voltage pulse electric field strength to 10kV / cm, pulse frequency to 1500Hz, and pulse width to 35μs , the flow rate was set to 2.0L / h under the treatment condition, and the high-voltage pulse electric field was turned on to treat all the sea cucumber protein slurry for 4 cycles to obtain a sea cucumber protein denatured slurry liquid, which was then introduced into the enzymatic hydrolysis tank and heated while stirring to make it Raise the temperature to 55°C, adjust the pH value to 10.5, add alkaline protease according to 4% of...

technical field

[0001] The invention belongs to the technical field of supplementary nutritional supplements for the elderly, and relates to a kind of 12 kinds of nutrient supplement factors that are stably formulated into marine source-sea cucumber immunoactive peptide powder, terrestrial plant source-soybean immunoactive peptide powder, terrestrial animal Source high-quality protein-skimmed milk powder 3 kinds of low-allergenic protein peptide-based carriers, and create a quality control technology for elderly supplementary food nutrition packages rich in low-allergenic heterologous immune active peptides. Background technique

[0002] "Nutrition package" is a kind of supplementary nutritional supplement made of soybeans, dairy products, etc. as food base materials, adding a variety of micronutrients and other auxiliary materials. According to the nutritional needs of different groups of people, it is usually divided into supplementary food nutrition packages for pregnant ...

Examples

Embodiment 1

[0040] (1) Preparation of hypoallergenic sea cucumber immunoactive peptide powder: the sea cucumber dry powder with protein content ≥ 30% is prepared with deionized water as the initial solution of sea cucumber protein with a protein concentration of 8%. After 15 minutes of homogenizer slurry treatment, remove the air bubbles on the surface of the sea cucumber protein slurry and pour it into the high-voltage pulse electric field feeding tank. Set the high-voltage pulse electric field strength to 15kV / cm, the pulse frequency to 2000 Hz, and the pulse width to 50μs, the flow rate is set to 2.5L / h treatment conditions, turn on the high-voltage pulse electric field to treat all the sea cucumber protein slurry for 6 cycles, and then prepare the denatured slurry liquid of sea cucumber protein, then introduce it into the enzymatic hydrolysis tank, and heat it while stirring. Heat up to 50°C, adjust the pH value to 9.5, add alkaline protease according to 2% of the added amount of sea c...

Embodiment 2

[0044] (1) Preparation of hypoallergenic sea cucumber immunoactive peptide powder: the sea cucumber dry powder with protein content ≥ 30% is prepared with deionized water as the initial solution of sea cucumber protein with a protein concentration of 5%. After 7.5 minutes of homogenizer slurry treatment, remove the air bubbles on the surface of the sea cucumber protein slurry and pour it into the high-voltage pulse electric field feeding tank. Set the high-voltage pulse electric field strength to 5kV / cm, the pulse frequency to 1000 Hz, and the pulse width to 20μs, the flow rate is set at 1.0L / h treatment conditions, turn on the high-voltage pulse electric field to process all the sea cucumber protein slurry for 2 cycles, and then prepare the denatured slurry liquid of sea cucumber protein, then introduce it into the enzymatic hydrolysis tank, and heat it while stirring. The temperature is raised to 52°C, the pH value is adjusted to 10.0, and alkaline protease is added according...

Embodiment 3

[0048] (1) Preparation of hypoallergenic sea cucumber immunoactive peptide powder: the sea cucumber dry powder with protein content ≥ 30% is prepared with deionized water as the initial solution of sea cucumber protein with a protein concentration of 7%. After the homogenizer is mixed for 5 minutes, remove the air bubbles on the surface of the sea cucumber protein slurry and pour it into the high-voltage pulse electric field feeding tank. Set the high-voltage pulse electric field strength to 10kV / cm, pulse frequency to 1500Hz, and pulse width to 35μs , the flow rate was set to 2.0L / h under the treatment condition, and the high-voltage pulse electric field was turned on to treat all the sea cucumber protein slurry for 4 cycles to obtain a sea cucumber protein denatured slurry liquid, which was then introduced into the enzymatic hydrolysis tank and heated while stirring to make it Raise the temperature to 55°C, adjust the pH value to 10.5, add alkaline protease according to 4% of...

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