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Degradation method of algal polysaccharides

A seaweed polysaccharide and seaweed extraction technology, which is applied in the field of marine chemical engineering, can solve the problems of unstable molecular weight of finished products, environmental pollution, large molecular weight, etc., and achieves the effects of good application prospect, process safety and simple operation.

Inactive Publication Date: 2021-06-08
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, seaweed polysaccharide has the characteristics of large molecular weight, poor water solubility and not conducive to body absorption, so its further degradation is conducive to its further development
[0003] In the prior art, CN 03138969.4 discloses a manufacturing method and application of marine sulfated polysaccharides. Alginic acid is made into a gel solution with water, and sulfuric acid is added to react for 4 to 6 hours. The supernatant is discarded by centrifugation, and the resulting precipitate is formulated Form an aqueous solution, adjust the pH value to form a flocculent precipitate, and precipitate the supernatant with ethanol, dissolve the precipitate with formamide, add chlorosulfonic acid for 6 to 8 hours and then precipitate with ethanol, and prepare the precipitate as an aqueous solution Membrane separation is used to obtain a marine sulfated polysaccharide solution with a molecular weight of 3000-5000, which is then spray-dried. However, acid degradation or oxidative degradation methods often require the use of a large amount of acid and alkali, which is likely to cause a certain degree of environmental pollution and affect the acid and alkali resistance of the preparation equipment. Corrosion requirements are also high; CN02147139.8 discloses sulfated algae polysaccharide oligosaccharides, the method adopts enzymatic degradation, although the enzymatic degradation reaction conditions are mild, the molecular weight uniformity of the degraded finished product is poor; in addition CN1267457C discloses a A preparation method of low molecular weight seaweed sulfated polysaccharide, by adding ascorbic acid and hydrogen peroxide to the seaweed sulfate polysaccharide solution for degradation reaction, the molecular weight of the degraded finished product is also unstable
So far, no literature has reported UV-binding H 2 o 2 Method for degrading seaweed polysaccharide

Method used

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  • Degradation method of algal polysaccharides
  • Degradation method of algal polysaccharides
  • Degradation method of algal polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Wash, after drying, the seaweed is ultrafinely pulverized, and 100 g is mixed with 400 ml 95 vol% ethanol solution, and then reflux 3 times in a micro-boiling state, a total of 5 h, then collect the extraction liquid to collect precipitation and dry, For the pre-treatment of seaweed ultrafine powder.

[0038] (2) Pre-treatment, the seaweed ultrafine powder is from 1 g: 50 mL ratio, using hot water method to extract the seaweed polysaccharide, extracting the extraction conditions at 100 ° C for 4 h, extracting liquid after centrifugation, evaporating the vacuum rotary evaporator Concentrated, then 95 vol% ethanol was slowly added to give the final concentration of 80% by weight, and the oscillation was mixed, and then placed at 4 ° C for 12 h, the next day, the resulting precipitate was washed with 95 vol% ethanol, after 25 ° C under conditions. Place, after ethanol, add the right amount of pure water recolition, and finally the vacuum rotary evaporator is concentrated and...

Embodiment 2

[0041] In addition to Hes with a concentration of 5 mmol / L 2 O 2 The aqueous solution is replaced with a concentration of 50 mmol / L 2 O 2 Outside the aqueous solution, the remaining and Example 1 were identical.

Embodiment 3

[0043] In addition to Hes with a concentration of 5 mmol / L 2 O 2 The aqueous solution is replaced with a concentration of 150 mmol / L 2 O 2 Outside the aqueous solution, the remaining and Example 1 were identical.

[0044] Molecular weight determination of the degradation product of Examples 1 to Example 3

[0045] Test of molecular weight: Take a proper amount of sample well dissolved in 0.02 mol / l kh 2 PO 4 In the aqueous solution, the spare was filtered with 0.22 μm aqueous phase filter. Columns: TSK-Gel G-3000PWXL (7.8mm × 300mm I.D, 7 μm), TSK-Gel G-6000PWXL (7.8mm × 300mm I.D., 13μm), two columns in series; mobile phase 0.02mol / LKH 2 PO 4 The flow rate was 0.5 ml / min, the column temperature was 35 ± 1 ° C, and the sample was 25 μL. As standards of different molecular weight dextran (including 4.32 kDa, 12.6kda, 126kda and 289kda), the standardized molecular weight measurement results are as follows figure 1 Indicated.

[0046] The molecular weight measurement result...

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Abstract

The invention provides a degradation method of algal polysaccharides, and belongs to the technical field of marine chemical engineering. The degradation method comprises the following steps: mixing algal polysaccharides, water and H2O2 to obtain a mixed solution; and carrying out radiation degradation on the mixed solution by adopting ultraviolet light to obtain a degradation product. According to the method, the algal polysaccharides are degraded by combining UV with H2O2, an acidic or alkaline solvent is not involved in the operation process, the process is safe and environmentally friendly, the cost is low, the operation is simple and time-saving, the degradation effect is relatively good, and the method has a good application prospect.

Description

Technical field [0001] The present invention relates to the field of marine chemical engineering, and more particularly to a degradation method of seaweed polysaccharides. Background technique [0002] Polysaccharide is a natural polymer carbohydrate composed of at least 10 monosaccharides, which can be divided into plant polysaccharides, animal polysaccharides, and microbial polysaccharides depending on their source. Algae polysaccharides are one of the important sources of plant polysaccharides. my country's common seaweed includes brown algae, red algae, green algae. Algae polysaccharides have a variety of bioactive, such as antioxidants, anticoagulants, antiviral, lower blood sugar and immunomodulation, etc., which gradually be focused on researchers. However, algae polysaccharide has a large molecular weight, poor water soluble and is not conducive to the body's absorption, so further degrading is conducive to it further development. [0003] In the prior art, CN 03138969.4 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/00
Inventor 游丽君陈孝勇李雄
Owner SOUTH CHINA UNIV OF TECH
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