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Preparation method of composite nanoparticles for increasing active oxygen content in tumor cells

A technology of composite nanoparticles and active oxygen content, which is applied in the field of tumor cells, can solve the problems of unsatisfactory treatment effect and weakened treatment effect, and achieve the effect of increasing active oxygen content, enhancing synergistic treatment, and increasing active oxygen content

Pending Publication Date: 2021-06-11
WUYI UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

In recent years, a treatment method targeting the tumor microenvironment—chemodynamic therapy—has just been proposed. Research on this treatment method can improve the therapeutic effect of tumors by reducing the pH value of the tumor microenvironment and supplementing the content of hydrogen peroxide, but its The treatment effect is not ideal
[0006] In addition, although the existing technology can enhance the therapeutic effect by changing the wavelength, improving the microenvironment of the tumor and increasing the oxygen content in the microenvironment, the excess glutathione in the microenvironment will quickly clear the photodynamic and chemical dynamics. Generated singlet oxygen and hydroxyl radicals to weaken the therapeutic effect of both

Method used

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  • Preparation method of composite nanoparticles for increasing active oxygen content in tumor cells
  • Preparation method of composite nanoparticles for increasing active oxygen content in tumor cells
  • Preparation method of composite nanoparticles for increasing active oxygen content in tumor cells

Examples

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Embodiment 1

[0041] Such as figure 1 As shown, this embodiment provides a method for preparing composite nanoparticles that increase the content of active oxygen in tumor cells, and the method includes the following steps:

[0042] S1), synthesis of UCNPs:

[0043] S101), adding 1 mmol of the aqueous solution of rare earth chloride into the reaction flask, and heating to 80° C. with magnetic stirring, until a white solid is precipitated;

[0044] S102), then add 15 mL of octadecene and 6 mL of oleic acid, heat to 130° C. to form a yellow homogeneous solution, and cool to room temperature;

[0045] S103), dropwise add 10mL methanol solution containing 4.0mmol ammonium fluoride and 2.5mmol sodium hydroxide to step S102) to form a yellow turbid solution;

[0046] S104), then heating the solution to 100° C., degassing for 15 minutes to remove methanol, water and oxygen, and then heating the solution to 300° C. under the protection of nitrogen, and keeping it for 1.5 h;

[0047] S105), after t...

Embodiment 2

[0057] In this example, in order to verify that UCNPs@Cu / ZIF-8 / RB / F127 can be degraded and released specifically by acid treatment, this example provides scans at different time points at different pHs, from figure 2 and image 3 It can be seen that the morphology of the crystals hardly changed under neutral environment (pH=7.4). However, when treated with an acidic solution, the crystal structure decomposed over time and degraded after 10 min of stirring, confirming the pH sensitivity of UCZRF.

[0058] Furthermore, the released Cu was detected by ICP 2+ As shown, when the pH value decreased to 6.5, more than 80% of Cu was released due to the decomposition of UCZRF 2+ . The above results indicated that UCNPs@Cu / ZIF-8 / RB / F127 has great potential as a drug for specific delivery.

[0059] The present invention uses diphenylisobenzofuran (DPBF) as a probe molecule to detect the extracellular singlet oxygen ( 1 o 2 )Such as Figure 4 (a) shown. 1 o 2 It can react with DP...

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Abstract

The invention provides a preparation method of composite nanoparticles for increasing the active oxygen content in tumor cells, which comprises the following steps: by synthesizing nanoparticles with core-shell structures, the nanoparticles firstly consume glutathione in a tumor microenvironment so as to avoid the removal of active oxygen; then, a photodynamic process and a further triggered chemical dynamic process are carried out under the irradiation of 980nm laser, so that the amount of active oxygen is accumulated, and an enhanced synergistic treatment effect is generated; according to the invention, the reversible reaction of a tumor microenvironment consumes glutathione and triggers continuous generation of hydroxyl radicals, so that the content of active oxygen is remarkably increased; under the irradiation of 980nm laser, a photodynamic process can be generated to generate singlet oxygen to damage tumor cells; and the effect of obviously increasing the content of active oxygen in tumor cells is achieved by adopting a photodynamic and chemical kinetics synergistic treatment mode, and the death probability of the tumor cells is further improved.

Description

technical field [0001] The invention relates to the technical field of tumor cells, in particular to a method for preparing a substance UCNPs@Cu / ZIF-8 / RB / F127 that can be used to increase the content of active oxygen in tumor cells. Background technique [0002] In recent years, tumor treatment strategies mediated by reactive oxygen species with strong oxidative properties have been extensively studied by researchers. Active oxygen includes superoxide anion (O 2- ), hydroxyl radicals (·OH) and singlet oxygen ( 1 o 2 ), which induce organelle damage, oxidative damage and cell death. [0003] Therefore, using high levels of reactive oxygen species to destroy the redox mechanism in tumor cells and cause tumor cell death is an effective means to inhibit tumor development. [0004] At present, there are two ways to generate reactive oxygen species: photodynamic (PDT) and chemical kinetic (CDT). Photodynamic has better spatial and temporal resolution, less trauma, and can be t...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K33/34A61K47/32A61K47/34A61K47/69A61P35/00B82Y5/00
CPCA61K41/0057A61K33/34A61K47/6949A61K47/34A61K47/32A61P35/00B82Y5/00A61K2300/00
Inventor 林君邹漫马平安陈叶青车海林
Owner WUYI UNIV
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