Bovine serum albumin-nano-silver modified chitosan nano-drug delivery system and preparation method thereof

A nanotechnology of bovine serum albumin and chitosan, which is applied in the field of polysaccharides, can solve the problems of toxicity, poor bioavailability of camptothecin, and easy drug resistance, so as to achieve controlled drug release, good therapeutic effect, and prevent tumors The effect of cell migration

Inactive Publication Date: 2021-06-18
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the bioavailability of camptothecin is very poor, and it is highly toxic to normal tissue cells, which is prone to drug resistance

Method used

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  • Bovine serum albumin-nano-silver modified chitosan nano-drug delivery system and preparation method thereof
  • Bovine serum albumin-nano-silver modified chitosan nano-drug delivery system and preparation method thereof
  • Bovine serum albumin-nano-silver modified chitosan nano-drug delivery system and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of nano-silver modified chitosan: add 1g of silver nitrate to 1000g of water, heat up to 50°C, add 20g of sodium ascorbate aqueous solution with a mass fraction of 5% drop by drop at a rate of 1 drop / second, and the addition is completed Stir for 2 hours, cool down to 20°C, add 10g of chitosan, dropwise add 50g of 2.5% acetic acid aqueous solution with a mass fraction of 2 drops / second, stir for 1 hour, dialyze with a dialysis bag with a molecular weight of 3500 for 1 day, freeze Dried nano-silver modified chitosan;

[0033] (2) Preparation of bovine serum albumin-nano-silver modified chitosan nano drug-loading system: take the nano-silver modified chitosan prepared in step (1), add it to 1500g water, heat up to 20°C, stir for 10 minutes, add 30g hydrochloric acid Berberine methanol solution (mass fraction of berberine hydrochloride is 5%), stirred for 0.5 hours, added 50g of camptothecin methanol solution (mass fraction of camptothecin is 2%), stirred f...

Embodiment 2

[0035] (1) Preparation of nano-silver modified chitosan: Add 5g of silver nitrate to 1300g of water, raise the temperature to 70°C, add 60g of sodium ascorbate aqueous solution with a mass fraction of 10% dropwise at a rate of 3 drops / second, and the addition is completed Stir for 3 hours, cool down to 40°C, add 20g of chitosan, dropwise add 150g of 10% acetic acid aqueous solution with a mass fraction of 4 drops / second, stir for 2 hours and dialyze with a dialysis bag with a molecular weight of 3500 for 3 days, freeze Dried nano-silver modified chitosan;

[0036] (2) Preparation of bovine serum albumin-nano-silver modified chitosan nano-loading system: take the nano-silver modified chitosan prepared in step (1), add it to 2000g water, heat up to 50°C, stir for 30 minutes, add 60g hydrochloric acid Berberine methanol solution (mass fraction of berberine hydrochloride is 15%), stir for 1.5 hours, add 150g camptothecin methanol solution (mass fraction of camptothecin is 6%), sti...

Embodiment 3

[0038] (1) Preparation of nano-silver modified chitosan: Add 2g of silver nitrate to 1100g of water, heat up to 60°C, add 40g of sodium ascorbate aqueous solution with a mass fraction of 7.5% drop by drop, at a rate of 2 drops / second, and the addition is complete Stir for 2.5 hours, cool down to 30°C, add 15g of chitosan, dropwise add 100g of acetic acid aqueous solution with a mass fraction of 6%, at a rate of 3 drops / second, stir for 1.5 hours and dialyze with a dialysis bag with a molecular weight of 3500 for 2 days, freeze Dried nano-silver modified chitosan;

[0039](2) Preparation of bovine serum albumin-nano-silver modified chitosan nano drug-loading system: take the nano-silver modified chitosan prepared in step (1), add it to 1700g water, heat up to 35°C, stir for 20 minutes, add 50g hydrochloric acid Berberine methanol solution (mass fraction of berberine hydrochloride is 10%), stir for 1 hour, add 90g camptothecin methanol solution (mass fraction of camptothecin is ...

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Abstract

The invention relates to the technical field of polysaccharides, in particular to a bovine serum albumin-nano-silver modified chitosan nano-drug delivery system and a preparation method thereof. Nano-silver chitosan gel is prepared from nano-silver modified chitosan, and the obtained gel has elasticity and stability similar to those of soft tissues, can effectively prolong the in-vivo circulation time of a nano-drug delivery system, and controls slow release of drugs. According to the invention, bovine serum albumin is also added, and the bovine serum albumin has excellent transmission and metabolism effects, good biocompatibility and degradability, can promote the circulation of the material in vivo, and more effectively controls the drug release. Two alkaloid drugs including berberine hydrochloride and camptothecin are added, camptothecin has an excellent anti-tumor effect, berberine hydrochloride has the effects of improving the microenvironment for tumor cell survival and preventing tumor cell migration, and the two drugs have a synergistic effect, so that the treatment effect on tumors is better.

Description

technical field [0001] The invention relates to the technical field of polysaccharides, in particular to a bovine serum albumin-nano-silver modified chitosan nano drug loading system and a preparation method thereof. Background technique [0002] Camptothecin is an alkaloid compound extracted from the plant Camptotheca, which has excellent antitumor activity and has good therapeutic effects on liver cancer, lung cancer, and colorectal cancer. However, the bioavailability of camptothecin is very poor, and it is highly toxic to normal tissue cells, and it is easy to develop drug resistance. Therefore, the nano-drug delivery system is of great significance for improving the anti-tumor activity of camptothecin and reducing its toxic and side effects. Such as Chinese patent CN112107690A discloses a kind of preparation method of camptothecin class drug nanoparticle, will have the camptothecin class drug of aromatic ring structure and copolymer to be dissolved in the organic solve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/52A61K31/4375A61K31/4745A61K47/54A61K47/52A61K47/36A61K47/04A61K47/42A61P35/00C08B37/08
CPCA61K9/5169A61K9/5161A61K9/5115A61K31/4745A61K31/4375A61K47/545A61K47/52A61P35/00C08B37/003A61K2300/00
Inventor 秦大伟王利振
Owner QILU UNIV OF TECH
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