Golden pomelo young fruit polysaccharide-containing grouper feed and preparation method thereof
A technology of grouper and young fruit, which is applied in the field of grouper feed containing pomelo young fruit polysaccharide and its preparation, can solve the problems of frequent outbreaks of grouper liver disease, insufficient disease prevention and control, and fish death economy , to achieve the effects of benefiting growth and reproduction, improving antioxidant capacity, and improving growth adaptability
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[0046] Example 1. Preparation and structural analysis of goldprised fruit polysaccharides
[0047] First, the preparation method of the gold pomelo, the preparation:
[0048] S1 gold pomelo is dry after drying, and deionized water is added to 1:30 according to the liquid ratio of 1: 30, and the raw rubber enzyme (2% w / v, pH 6.0, 50 ° C) is 1.5-3H. ;
[0049] After S2, after the product acquired by step S1, it is extracted by ultrasonic breakage;
[0050] After the extraction of the S3 ultrasonic breakage, the extract is centrifuged and concentrated, and trichloroacetic acid is removed, and the four-fold volume of ethanol precipitated overnight layers under 4 ° C, and precipitated;
[0051] S4 is dissolved in deionized water and injects Deae Sepharose Fast Flow, and the deAsepharose Fast Flow packing first uses 0.5 mol / ml hydrochloric acid for 1 hour, washing the impurities in the filler, with 4-5 times volume The distilled water was washed away from neutral, the resolution flo...
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