Preparation method of plant functional protein and application of plant functional protein in protection of acute alcoholic liver injury

A functional and plant technology, which is used in the preparation of plant functional proteins and the application field of protection of acute alcoholic liver injury, can solve the problems of inappropriateness, long treatment period, and accompanying adverse reactions.

Pending Publication Date: 2021-07-06
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The treatment cycle of chemical drugs is short, but it is often accompanied by adverse reactions, and it is not suitable for the elderly, weak and pregnant people, and there are certain limitations
The side effects of traditional Chinese medicine are small but the treatment cycle is long. Except for some Chinese patent medicines that have been verified, other prescriptions vary from person to person, which is not conducive to large-scale clinical application

Method used

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  • Preparation method of plant functional protein and application of plant functional protein in protection of acute alcoholic liver injury
  • Preparation method of plant functional protein and application of plant functional protein in protection of acute alcoholic liver injury
  • Preparation method of plant functional protein and application of plant functional protein in protection of acute alcoholic liver injury

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Experimental program
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Effect test

Embodiment 1

[0024] A method for preparing plant functional protein, comprising the following preparation steps: adding defatted wheat germ powder into NaCl solution at a ratio of 1:10 w / v (mass volume ratio), stirring evenly, and shaking at a constant temperature; Supernatant: Using a Buchner funnel with a vacuum pump, pass the supernatant through 0.22 μm and 0.1 μm microporous membranes in sequence; collect the filtrate with a dialysis bag, and obtain a plant functional protein solution after magnetic stirring.

[0025] The mass concentration of the NaCl solution is 0.9%.

[0026] The constant temperature shaking time is 1.5h, and the temperature is 30°C.

[0027] The centrifugation speed is 4000rpm, the centrifugation temperature is 20°C, and the centrifugation time is 10min.

[0028] The MWCO of the dialysis bag is 3500.

[0029] The time of magnetic stirring was 12 h, and the temperature was 4°C.

[0030] The plant functional protein of the invention can be applied to protect the l...

Embodiment 3

[0064] organ index

[0065] Organ coefficient = organ weight (g) / mouse final body weight (g) × 100%.

[0066] Changes in organ indexes can initially reflect the degree of alcohol-mediated damage to the body. Liver index, as the main indicator in this change, is the ratio of the wet weight of the mouse liver to its body weight. Alcohol-mediated liver damage is mainly manifested by cell damage and lipid metabolism disorder. If the liver becomes fatty, the ratio of wet weight to body weight will increase. It can be seen from the data in Table 2 that the liver coefficient of the model group was significantly higher than that of the blank group, while the liver coefficient of the WEG treatment group was significantly lower than that of the model group and had no significant difference from the blank group (P<0.05). Among them, the effect of middle dose was significantly better than that of high dose treatment group. Correspondingly, compared with the blank group mice, the spleen...

Embodiment 4

[0071] blood fat

[0072] The levels of TC and TG in the serum of acute ALD mice were detected by a biochemical automatic analyzer.

[0073] Lipid metabolism disorder is one of the manifestations of alcoholic liver injury. Alcohol promotes the synthesis of TC and TG, transfers high-density lipoprotein from the liver to the periphery, increases the liver lipid content, and leads to the deterioration of ALD. The severity of liver fat can be judged by detecting the content of TC and TG in serum. Such as figure 1 As shown, the serum TC and TG levels of the mice in the model group were significantly higher than those in the blank group, and under the protective effect of wheat germ globulin, the blood lipid levels of the mice with liver injury were significantly lower than those in the model group (P<0.05). It can be seen that WEG can play a very good protective role against alcohol-mediated fatty liver lesions.

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PUM

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Abstract

The invention discloses a preparation method of a plant functional protein and application of the plant functional protein in protection of acute alcoholic liver injury, and belongs to the technical field of functional materials. Natural defatted wheat germ powder is used as a raw material to prepare wheat germ globulin through salt extraction, and the wheat germ globulin can effectively reduce the increase in liver indexes, spleen indexes and kidney indexes caused by acute alcohol stimulation, reduce the levels of total cholesterol and triglycerides in serum and play a role in protecting the liver, improve the content of glutathione in the liver and the activity of superoxide dismutase, reduce the content of malondialdehyde, improve liver oxidative stress caused by alcohol, relieve the degree of hepatocyte necrosi and play a protective effect on acute alcoholic liver injury. Compared with the existing anti-alcohol and liver-protecting medicine, the wheat germ globulin disclosed by the invention is safer and more effective, has no toxic or side effect, and obviously improves physiological and biochemical abnormalities caused by alcohol. The wheat germ globulin has considerable development and application values in the aspect of improving the acute alcoholic liver injury, and has a wide market application prospect.

Description

technical field [0001] The invention belongs to the technical field of functional materials, and in particular relates to a preparation method of plant functional protein and its application in protecting acute alcoholic liver injury. Background technique [0002] Alcoholic liver disease (ALD) will not only damage the health of drinkers, but also endanger the family and cause social problems. Statistics show that in 2016, excessive drinking caused about 3 million deaths worldwide. One-time excessive drinking or long-term heavy alcohol intake can lead to liver damage, which can be divided into chronic and acute liver damage according to different causes. It is generally believed that alcoholic liver injury is caused by oxidative stress mediated by ethanol and its metabolites, immune response and liver cell damage. [0003] The initial stage of alcoholic liver injury is fatty liver, which is mainly due to the accumulation of fat (triglycerides, phospholipids and cholesterol ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/14C07K1/34A61K38/16A61P1/16
CPCA61K38/00A61P1/16C07K14/415
Inventor 黄继红廖爱美魏兆军侯银臣潘龙董得平张康逸罗登林刘劝平杨璨睿
Owner HENAN UNIVERSITY OF TECHNOLOGY
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