Semi-homogeneous metalloenzyme integrated nano-catalyst as well as preparation method and application thereof

Abstract

The invention relates to a semi-homogeneous metalloenzyme integrated nano-catalyst as well as a preparation method and application thereof. The semi-homogeneous metalloenzyme integrated nano-catalyst comprises palladium metal nanoparticles wrapped by a hydrophobic organic molecular cage and lipase CALB fixed on the surface of the organic molecular cage. According to the semi-homogeneous metalloenzyme integrated nano-catalyst, the hydrophobic organic molecular cage is used as a stabilizer and a carrier of immobilized enzyme, separation of a metal catalyst and enzyme can be achieved, and contact inhibition of the metal catalyst and the enzyme is avoided. Meanwhile, the organic molecular cage can provide extremely high hydrophobicity for the catalyst, and the solubility of the catalyst in an organic solvent is improved, so a semi-homogeneous catalyst can be realized, the mass transfer performance of the catalyst is improved, and the catalytic capability of the catalyst can be effectively improved.

Description

technical field [0001] The invention relates to the technical field of nanocatalysis, in particular to a semi-homogeneous metalloenzyme-integrated nanocatalyst. At the same time, the invention also relates to a preparation method of a semi-homogeneous metalloenzyme-integrated nanocatalyst, and the semi-homogeneous metalloenzyme-integrated nanocatalyst. Catalyst application. Background technique [0002] The field of chemical-biological catalysis has the advantages of both chemical catalysts and biocatalysts, and has played a huge role in the fields of organic synthesis and fine chemical industry. However, there is usually mutual inhibition between chemical catalysis and biocatalysis, which seriously hinders the development of both. combination. Although heterogeneous metalloenzyme-integrated catalysts can effectively solve the problem of metal-enzyme mutual inhibition, the mass transfer problem caused by the inherent nature of heterogeneous catalysts will lead to their low ...

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Problems solved by technology

[0002] The field of chemical-biological catalysis has the advantages of both chemical catalysts and biocatalysts, and has played a huge role in the fields of organic synthesis and fine chemical industry. However, there is usually mutual inhibition between chemical catalysis and biocatalysis, which seriously hinders the development of both. combination of

Although heterogeneous metalloenzyme-integrated catalysts can effectively solve the problem of metal-enzyme mutual inhibition, the mass transfer problem caused by the inherent nature of heterogeneous catalysts will lead to their low catalytic ability.

[0003] Chiral amines are important intermediates in the synthesis of drugs. The traditional kinetic resolution of chiral amines catalyzed by lipase is one of the effective ways to obtain single enantiomers of chiral amines, but the maximum yield of this method is low

Although the current metalloenzyme cascade catalytic reaction can improve the yield of traditional kinetic resolution, there are still many shortcomings as follows: (1) There is mutual inhibition between the metallocatalyst and the biocatalyst; (2) Relative to the lipase Appropriate reaction temperature, a higher reaction temperature not lower than 70°C is required; (3) Basic additives need to be added to inhibit the occurrence of side reactions

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