Method for rapidly detecting histamine

A kind of histamine and fast technology, applied in the field of rapid detection of histamine, using molecular imprinting-surface enhanced Raman spectroscopy combined technology to rapidly detect histamine in fish meat, which can solve the problem of complex matrix components and affect the combination of chemical molecules to be tested and substrates. , affecting the sensitivity of detection results, etc., to achieve high selection specificity, reduce interference of SERS signals, and high accuracy

Pending Publication Date: 2021-07-09
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aquatic products, especially fish, have complex matrix components. Substances such as protein, fat, and water will affect the combination of chemical molecules to be tested and the substrate, thereby affecting the sensitivity of the test results.

Method used

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  • Method for rapidly detecting histamine
  • Method for rapidly detecting histamine
  • Method for rapidly detecting histamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The Raman spectrum detection of embodiment 1 histamine solid standard substance

[0049] Weigh about 0.5 mg of histamine standard solid powder and place it on a glass slide, press into tablets, and detect by Raman spectroscopy. The result is as figure 1 As shown, the main characteristic peaks of histamine are located at: 650, 670, 814, 928, 984, 1032, 1098, 1126, 1174, 1251, 1293, 1359, 1433, 1479 and 1596cm –1 , and can be clearly identified.

Embodiment 2

[0050] Example 2 Preparation of gold core silver shell nano sol (Au@AgNPs)

[0051] (1) Adding 1.8mL concentration of 1 wt% trisodium citrate aqueous solution to the 0.01% chloroauric acid solution to the heated and boiled 100mL concentration, while vigorously stirring at 1100rpm and keeping boiling for 5min, then quickly ending the reaction in an ice-water bath to prepare The particle size of the obtained gold nanoparticles is 18±2nm, which is used as the gold seed solution.

[0052] (2) Take 3 mL of gold seed solution and add 0.4 mL of L-ascorbic acid with a concentration of 0.1 mol / L, and add 2.7 mL of silver nitrate solution with a concentration of 1 mmol / L dropwise while stirring. After magnetic stirring for 5 min, gold-core silver-shell nanoparticles were prepared and used as a substrate for surface-enhanced Raman spectroscopy for subsequent detection.

[0053] The prepared nano-substrates were detected, and the TEM results were as follows: figure 2 As shown, the aver...

Embodiment 3

[0060] Example 3 Preparation of histamine molecularly imprinted polymer

[0061] Add 0.13mmol template molecule histamine and 0.65mmol functional monomer methacrylic acid into a 250mL three-necked flask, dissolve in 20mL acetonitrile after ultrasonication for 5 minutes, and pre-polymerize for 20 minutes with magnetic stirring; then add 3.28mmol cross-linking agent dimethacrylic acid Ethylene glycol ester is dispersed in the above pre-polymerization mixture by ultrasonic for 30 minutes, 20 mg of initiator 2,2-azobisisobutyronitrile is added, ultrasonically degassed for 5 minutes, and nitrogen protection is introduced for 30 minutes, and the three-neck flask is placed at a constant temperature of 60°C In a water bath, the reaction was carried out with magnetic stirring for 24 hours. The obtained molecularly imprinted polymer was properly ground (sieved through a 200-mesh molecular sieve) and then washed with a mixed solution of methanol and acetic acid (volume ratio 9:1), placed...

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Abstract

The invention belongs to the field of food safety detection, and provides a method for rapidly detecting histamine, the method comprises the following steps: by taking gold-core silver-shell nano sol as an enhanced substrate, detecting a sample by applying a molecularly imprinted polymer capable of selectively adsorbing histamine in combination with a surface enhanced Raman spectroscopy technology, and obviously responding to SERS signal change of low-concentration histamine in the sample. The method can be used for detecting trace histamine in aquatic products such as fish meat. The detection method can simply, quickly and accurately realize qualitative and quantitative detection of histamine, and is good in sensitivity and high in selectivity.

Description

technical field [0001] The invention belongs to the field of food safety detection, and specifically relates to a method for rapidly detecting histamine, in particular to a method for rapidly detecting histamine in fish using molecular imprinting-surface-enhanced Raman spectroscopy (SERS) combined technology. Background technique [0002] Histamine, also known as histamine, has the molecular formula C 5 h 9 N 3 , the chemical name is 4(5)-(2-aminoethyl)imidazole. Histamine is a biogenic amine, and also a polyamine substance that has the greatest impact on human health. It is mainly produced by histidine in organisms through the decarboxylation reaction of histidine decarboxylase. The content of histamine in fresh aquatic products is low, but when the fish meat is improperly stored and rotten, a large amount of histamine will be produced. Therefore, both foreign countries and my country have formulated the maximum residue limit standard for histamine in aquatic products a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658G01N21/65G01N2021/655
Inventor 赖克强张苑怡宁海花苗军舰曲映红
Owner SHANGHAI OCEAN UNIV
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