A kind of composite nanomaterial with antibacterial effect and its preparation method and application

A composite nanomaterial and nanomaterial technology, which is applied in the field of composite nanomaterials and its preparation for removing root canal bacteria in teeth, can solve problems that have not been reported, and achieve good results, easy control of process parameters, and stable performance

Active Publication Date: 2022-05-13
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • A kind of composite nanomaterial with antibacterial effect and its preparation method and application
  • A kind of composite nanomaterial with antibacterial effect and its preparation method and application
  • A kind of composite nanomaterial with antibacterial effect and its preparation method and application

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Experimental program
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Effect test

Embodiment

[0045] Embodiment. Preparation and characterization of antibacterial material

[0046] 1. Preparation of antibacterial materials

[0047] The preparation method of the mesoporous silicon composite material loaded with protoporphyrin and ferric chloride described in this embodiment comprises the following steps:

[0048] (1) Synthesis of nano-mesoporous silicon carrier: Dissolve 10 g of cetyltrimethylammonium chloride (CTAC) and 0.4 g of triethanolamine (TEA) in 100 mL of deionized water, and stir vigorously at 95 ° C for 1 h , and then slowly and uniformly dropwise added 7.5mL Tetraethyl orthosilicate (TEOS) and then stirred for 1h, and the resulting solution was centrifuged at 11000rpm for 30 minutes to collect the precipitate, which was washed several times with deionized water and methanol. The resulting precipitate was mixed with concentrated hydrochloric acid, vigorously stirred at 60 °C under reflux for 48 hours to remove the template CTAC, and then obtain mesoporous si...

experiment example

[0055] Experimental example. Material performance test

[0056] 1. ROS production efficiency test

[0057] The non-fluorescent 2,7-dichlorofluorescein diacetate (DCFH-DA) was used as a fluorescent probe to detect the ROS generation of the synthesized materials. Dissolve 0.1 mg of the prepared MSN, M@P and M@P-Fe in 1 mL of deionized water or 0.01% V / VH 2 o 2 , and then 20uL of DCFH-DA was added, and the samples of the ultrasound group (US) were exposed to ultrasound conditions (1MHz, 0.5W / cm 2 , after a duration of 3min), use a fluorometer to detect the fluorescence intensity of DCF under excitation at a wavelength of 488nm, and the detection results are as follows Figure 3-4 As shown, for easy identification, Figure 4 for image 3 Partial zoom-in.

[0058] Figure 3-4 Among them, the order of fluorescence intensity peaks from high to low is: M@P-Fe+H 2 o 2 +US, M@P-Fe+H 2 o 2 、M@P+H 2 o 2 +US、MSN+H 2 o 2 +US、M@P+H 2 o 2 、MSN+H 2 o 2 , M@P+US, M@P-Fe+US, M...

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Abstract

The invention discloses a composite nano-material with sterilization effect and its preparation method and application. The mesoporous nano-material is modified by amination and loaded with a sound sensitizer, and the surface is coated with divalent or / and ferric ions. In the presence of hydrogen peroxide, the Fenton reaction occurs to generate reactive oxygen species (ROS) to play a bactericidal effect. By using ultrasonic auxiliary methods commonly used in oral diagnosis and treatment, it can stimulate the sound sensitizer to generate ROS, and at the same time further assist and enhance the Fenton reaction, generate a large amount of ROS, improve the efficiency of removing bacterial infection and sterilization in the root canal, and achieve the removal of bacterial biofilm in the root canal And the purpose of completely killing bacteria.

Description

technical field [0001] The invention relates to the technical field of nanomaterials, in particular to a composite nanomaterial for removing tooth root canal bacteria and its preparation method and application. Background technique [0002] Pulpitis and periapical infection are common and high-incidence diseases in the oral cavity at present, which not only easily lead to oral diseases such as tooth loss, dentition defect, oral and maxillofacial infection, but also cause bacterial infection such as pulp and periapical infection. Systemic diseases such as endocarditis not only cause great damage to the physical and mental health of patients, reduce the quality of life of patients, but also cause huge economic losses. According to current research, bacterial infection is the main cause of pulpitis, and root canal therapy is currently the first choice for the treatment of pulpitis and periapical lesions internationally recognized. Disinfect, and then tightly fill the root cana...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K6/52A61K6/69A61K41/00A61P31/04B82Y5/00
CPCA61K6/52A61K6/69A61K41/0033A61P31/04B82Y5/00
Inventor 张先正郭景梅黄翠刘妙登
Owner WUHAN UNIV
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