Crassostrea gigas DM9 structural domain-containing recombinant protein rCgDM9CP-6, and preparation method and application thereof

A technology of recombinant protein, long oyster, applied in the field of molecular biology
CN113185593APending Publication Date: 2021-07-30DALIAN OCEAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN OCEAN UNIV
Publication Date
2021-07-30

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Abstract

The invention discloses a crassostrea gigas DM9 structural domain-containing recombinant protein rCgDM9CP-6. The amino acid sequence of the crassostrea gigas DM9 structural domain-containing recombinant protein rCgDM9CP-6 is as shown in SEQ ID NO. 1; A preparation method of the recombinant protein rCgDM9CP-6 sequentially comprises the following steps: carrying out PCR amplification on a Crassostrea gigas CgDM9CP-6 gene coding region fragment by using primers P1 and P2, wherein the DNA sequence of the primer P1 is as shown in SEQ ID NO.2, and the DNA sequence of the primer P2 is as shown in SEQ ID NO.3; carrying out BamHI and Xhol I enzyme digestion on a PCR amplification product and a pET30a vector, then connecting and transforming through T4 ligase, and sequencing to identify a recombinant; and transferring the recombinant into an escherichia coli Transsetta (DE3) expression strain, performing induced culture, and then performing purification and renaturation to obtain the recombinant protein rCgDM9CP-6 with the amino acid sequence in the sequence table SEQ ID NO.1. The recombinant protein rCgDM9CP-6 can be applied to preparation of drugs for inhibiting gram-positive bacteria M.luteus.
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Description

technical field

[0001] The invention belongs to the technical field of molecular biology, and in particular relates to rCgDM9CP-6, a recombinant protein containing a DM9 domain of oyster, a preparation method and an application. Background technique

[0002] long oysters ( Crassostrea gigas ) is an important marine cultured shellfish. Due to the lack of adaptive immune defense system of long oyster, it mainly relies on the innate immune system to resist the infection of exogenous pathogenic microorganisms. Due to the deterioration of the marine environment in recent years, various diseases caused by bacteria, fungi and viruses have continued to break out in oyster culture groups, causing huge economic losses.

[0003] DM9 is a protein that contains a carbohydrate recognition domain (DM9) and can resist fungal and bacterial infections, promote cell phagocytosis and perform immune regulation functions. Studies have found that DM9 domain-containing proteins play an important...

Claims

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