Method for determining aflatoxin B1 in traditional Chinese medicinal materials
A technology for the determination of aflatoxin and aflatoxin B1 in Chinese medicinal materials, which can solve the problems of poor accuracy, inaccurate quantification, and complicated operation of the detection method, and achieve high precision
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Embodiment 1
[0024] Aflatoxin B in Chinese herbal medicines 1 The assay method is characterized in that: comprising,
[0025] Sample selection: This operation is used to select the material to be tested. Grind about 200g of Chinese herbal medicine into powder with a pulverizer to make the particle size smaller than 2mm test sieve. After mixing evenly, divide it into 50g, store it in a sample bottle, and keep it airtight. Save for testing; weigh 1g of Chinese herbal medicine sample (accurate to 0.01g) and put it in a 50mL centrifuge tube, add 20mL of 50% methanol aqueous solution, vortex extract for 3 minutes, centrifuge at 4000r / min for 5 minutes, and use the supernatant for later use , It should be noted that when encountering a medicinal sample with heavy pigment, accurately pipette 10mL of the supernatant extract into a 15mL centrifuge tube, add 300mg of C18 powder, 900mg of anhydrous magnesium sulfate, 300mg of PSA powder, 300mg of silica gel, 90mg of GCB, Vortex for 3 minutes, centri...
Embodiment 2
[0031] The aflatoxin B 1 The preparation of immunofluorescence test strips is to use bioinformatics technology to prepare aflatoxin B 1 Antigen and antibody of the aflatoxin B1 antibody and fluorescent microspheres were coupled, and the test strip was prepared by indirect competition method, and the fluorescent microsphere-conjugated antibody was sprayed and fixed on the fiber pad, and antigen and secondary antibody of appropriate concentration were used in the NC Stretch on the membrane, embed and fix the antigen and secondary antibody on the NC membrane, age at 37° for 24 hours, the precision is improved, the quality control line C line and the detection line T line are formed, and the concentration of aflatoxin B1 in the sample is taken as the horizontal line Coordinates, the corresponding concentration T / C fluorescence intensity ratio is the ordinate, draw a multi-segment linear standard curve, the unknown concentration sample can read the T / C value through the fluorescenc...
Embodiment 3
[0042] The setting of the multi-segment linear standard curve weighs 6 samples with a mass of 1.00g and places them in 6 50mL centrifuge tubes. 1 Accurately pipette 0 μL, 10 μL, 25 μL, 50 μL, 100 μL, and 150 μL from the standard solution into the above-mentioned 6 centrifuge tubes; read the above-mentioned 6 spiked samples according to the methods of sample selection, test strip selection, liquid extraction and data acquisition. For the T / C ratio, take the spiked concentration as the abscissa and the T / C ratio as the ordinate, establish a multi-segment linear standard curve and input it into the fluorescence card reader.
[0043] serial number Punctuation concentration (μg / kg) T / C value 1 0 1.12 2 2.00 0.95 3 5.00 0.74 4 10.0 0.51 5 20.0 0.33 6 30.0 0.24
[0044] This table is the tested T / C value in six centrifuge tubes, and plotted as figure 1 The multi-segment linear standard curve diagram shown, the centrifuge tube is ...
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