Streptomyces lateritius A001 and application thereof
A technology of Streptomyces rubrum and bacterial agent, which is applied in the field of microorganisms to achieve the effect of prolonging lifespan and relieving aging
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Embodiment 1
[0036] Example 1 Isolation and screening of Streptomyces lateritius A001
[0037] 1. Material collection: Sand samples were collected from Miyun District, Beijing, China, mixed and put into sterilized ziplock bags, sent to the laboratory at room temperature on the same day, and stored at 4 °C for later use.
[0038] 2. Sample pretreatment: air-dry the soil sample at room temperature for 20 days, and sieve it for later use. Weigh 1 g of the air-dried sample, grind it in a mortar and grind it at 100 °C for 1 h, and put the treated soil sample into a solution containing 1% Na 4 PO 3 100 mL of sterile distilled water was shaken for 30 min, and then treated with an ultrasonic cleaner for 40 s, and the soil suspension was used to separate strains.
[0039] 3. Isolation and purification of strains: Take the treated soil suspension for gradient dilution, absorb the stock solution and 10 -1Dilute the solution to the surface of the solid medium (set up 3 replicates and 1 blank contro...
Embodiment 2
[0041] Example 2 Identification of Streptomyces lateritius A001
[0042] 1. Morphological and Physiological Biochemical Identification
[0043] After the bacterial strain Streptomyces lateritius A001 screened in Example 1 was grown on YIM38 agar medium for 7 days, the hyphae in the substrate developed well, without septa, and did not break; the aerial hyphae grew well and were multi-branched. ; Spore filaments are straight and flexible, and the spores are round or oval and smooth ( figure 1 ), the colony morphology see figure 2 , these features are consistent with Streptomyces.
[0044] Physiological and biochemical identification was carried out referring to the relevant contents of "Rapid Identification and Isolation of Actinomycetes" and "Bergey'Manual of Systematic Bacteriology" Vol. II, and the results are shown in Table 1.
[0045] Table 1 Physiological and biochemical characteristics of strain Streptomyces lateritius A001
[0046]
[0047] 2. Molecular identific...
Embodiment 3
[0053] Example 3 Preparation of Streptomyces lateritius A001 anti-aging preparation for Caenorhabditis elegans
[0054] A single colony of Streptomyces lateritius A001 was selected and inoculated into 150 mL of YIM38 liquid medium, and cultured with shaking at 200 rpm and 28°C for 48 h. After the culture solution was centrifuged at 4 °C and 9500 rpm for 20 min, the fermentation supernatant and cells were collected respectively. The fermentation supernatant was extracted with an equal volume of ethyl acetate, and the bacteria were extracted with an equal volume of acetone, and then the extract was concentrated by rotary evaporation at 38°C with a rotary evaporator (RE-52AA, YARONG, China). , the concentrate was redissolved in 1 mL of methanol to obtain the ethyl acetate extract of the fermentation supernatant of the strain A001 and the acetone extract of the bacteria, respectively, and stored at -20 °C for future use.
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