Production method of trichoderma chlamydospore

A technology for chlamydospore and production method, which is applied in the field of fermentation engineering, can solve the problems of affecting the quality of chlamydospore hyphae, can not meet the lack of carbon source, affect the activity and yield of spores, etc., and achieves storage resistance and strong stress resistance. , the effect of cheap and easy-to-obtain raw materials

Pending Publication Date: 2021-08-06
上海大井生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this type of method is still a one-time feeding, which cannot meet the lack of carbon source in the carbon source fermentation process, which will affect the quality of mycelium forming chlamydospores, and then affect the activity and output of spores

Method used

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  • Production method of trichoderma chlamydospore
  • Production method of trichoderma chlamydospore
  • Production method of trichoderma chlamydospore

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Medium ratio:

[0033] a) Potato glucose culture solution:

[0034] 200g potato extract (200g potato peeled, chopped, boiled until soft, then filtered with gauze to get the clear liquid), 20g glucose, add water to 1L

[0035] Add 1.5% (15g / L) agar to the potato dextrose agar plate on the basis of the above, and sterilize at 121°C for 25min.

[0036] b) cornmeal medium,

[0037]

[0038]

[0039] After mixing in the fermenter, sterilize at 121°C for 30 minutes, cool to 28°C for inoculation after sterilization, and the pH value after sterilization is 5.0.

[0040] S1. Strain maintenance and seed liquid culture: Aseptically operated in a clean environment, Trichoderma harzianum strains were maintained on potato dextrose agar plates. Punch a hole on the flat plate covered with thalline and inoculate it into 500mL potato dextrose culture solution, at 28 DEG C, 120rpm shaker shaking culture for 48h, can be used for inoculating the primary fermenter (seed tank).

[0...

Embodiment 2

[0058] Medium ratio:

[0059] a) Potato glucose culture solution:

[0060] 200g potato extract (200g potato peeled, chopped, boiled until soft, then filtered with gauze to get the clear liquid), 20g glucose, add water to 1L

[0061] Add 1.5% (15g / L) agar to the potato dextrose agar plate on the basis of the above, and sterilize at 121°C for 25min.

[0062] b) cornmeal medium,

[0063] corn flour 60g / L Sodium chloride NaCl 1g / L Sodium nitrate NaNO 3

1.42g / L Potassium dihydrogen phosphate KH 2 PO 4

3.82g / L Ammonium sulfate (NH 4 ) 2 SO 4

1.1g / L Magnesium Sulfate Heptahydrate MgSO 4 ·7H 2 o

0.5g / L Zinc sulfate heptahydrate ZnSO 4 ·7H 2 o

0.002g / L Heptahydrate ferrous sulfate FeSO 4 ·7H 2 o

0.0075g / L Manganese Sulfate Monohydrate MnSO 4 ·H 2 o

0.0025g / L

[0064] After mixing in the fermenter, sterilize at 121°C for 30 minutes, cool to 30°C for inoculation after steri...

Embodiment 3

[0082] Medium ratio:

[0083] a) Potato glucose culture solution:

[0084] 200g potato extract (200g potato peeled, chopped, boiled until soft, then filtered with gauze to get the clear liquid), 20g glucose, add water to 1L

[0085] Add 1.5% (15g / L) agar to the potato dextrose agar plate on the basis of the above, and sterilize at 121°C for 25min.

[0086] b) cornmeal medium,

[0087] corn flour 60g / L Sodium chloride NaCl 1g / L Sodium nitrate NaNO 3

1.42g / L Potassium dihydrogen phosphate KH 2 PO 4

3.82g / L Ammonium Sulfate (NH 4 ) 2 SO 4

1.1g / L Magnesium Sulfate Heptahydrate MgSO 4 ·7H 2 o

0.5g / L Zinc sulfate heptahydrate ZnSO 4 ·7H 2 o

0.002g / L Heptahydrate ferrous sulfate FeSO 4 ·7H 2 o

0.0075g / L Manganese Sulfate Monohydrate MnSO 4 ·H 2 o

0.0025g / L

[0088] After mixing in the fermenter, sterilize at 121°C for 30 minutes, cool to 28°C for inoculation after steri...

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Abstract

The invention provides a production method of trichoderma chlamydospore, and belongs to the technical field of fermentation engineering. The method comprises the following steps: activating a trichoderma harzianum strain, performing inoculating in a primary seed tank for seed amplification, further performing transferring to a secondary seed tank for further fermentation amplification, performing transferring to production fermentation equipment for segmented fermentation, supplementing the material for the first time in the process, then supplementing alkali, controlling the pH value, supplementing the material for the second time when the pH value is stabilized to be 3-4 and chlamydospores start to form in the fermentation process, performing fermenting until the chlamydospores are mature, performing discharging from a tank, and performing treating to obtain the trichoderma chlamydospores. According to the method, the technical problem of low chlamydospore yield is solved through multi-time feeding and segmented fermentation, and the yield of live spores in fermentation liquor during large-scale fermentation can reach (1.5-2.5) * 10 < 8 >/mL. The spores are filtered and separated by adding a solid carrier, and the number of live spores in the prepared spore powder can reach (10-16) * 10 < 8 >/g and is far higher than the related standard.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a production method of Trichoderma chlamydospores. Background technique [0002] Due to the threat of agricultural non-point source pollution caused by excessive use of chemical pesticides and fertilizers to the ecological environment and the sustainable development of agriculture, biological control technology has gradually received attention. Trichoderma, as a commonly used plant disease biological control fungus in the world, has become an important resource microorganism for the development of biopesticides and biofertilizers. However, as active biological agents, the stability and shelf life of Trichoderma biopesticides and biofertilizers are important factors restricting their application. [0003] Chlamydospore is a survival structure with strong stress resistance formed by Trichoderma in unfavorable environment. Inducing Trichoderma to produce chlamydosp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12N1/14C12R1/885
CPCC12N3/00C12N1/14
Inventor 唐卫东张学力陈捷李易
Owner 上海大井生物工程有限公司
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