Composite material for repairing articular cartilage injury and preparation method thereof

Abstract

The invention relates to a composite material for repairing articular cartilage injury. The composite material comprises liquid collagen prepared from animal skin tissues, PRP (platelet-rich protein) prepared from autologous blood and having a certain number of platelets, and a calcium ion activator with the mass percent concentration of 5-10%, wherein the collagen, the PRP and the calcium ion activator are mixed in a volume ratio of (10-15): (10-15): 1, and the collagen and the PRP are the same in volume. The invention also discloses a preparation method of the composite material. The preparation method comprises the following steps: sequentially preparing the collagen and the PRP, mixing the collagen and the PRP, and then mixing the calcium ion activator. The preparation method disclosed by the invention is simple and easy to operate, does not need complex equipment, and is wide in raw material source; and by selecting the animal skin tissues and the autologous blood of a patient, the cost can be obviously reduced, exogenous thrombin does not need to be added, the probability of adverse reactions is reduced, and release of growth factors in the PRP can be slowed down.

Description

Technical field [0001] The present invention relates to the field of medical materials, in particular a composite material for repairing articular cartilage damage to the art. Background technique [0002] The joint cartilage is a milky white, smooth transparent tissue composed of cartilage cells and cartilage matrix, covering the joint surface, which can ensure normal motion of the body. The joint cartilage damage is a more common joint surgery, which can be caused by trauma, osteoarthritis, rheumatoid arthritis and exfoliated osteoclastitis. Unexpected treatment is easy to develop into knee-proof arthritis (KOA), and later Leading pain, joint malformation until disability, seriously affecting patient movement function and quality of life while aggravating patient psychological pressure or spiritual burden. [0003] With the continuous development of renewable medical and tissue engineering, new methods and ideas are provided for cartilage repair and reconstruction. The cartilag...

AI Technical Summary

Problems solved by technology

[0004] With the clinical popularization of PRP treatment of KOA, there are still some shortcomings: first, the density of PRP network structure is determined by the amount of its raw material fibrinogen, and its type depends on the total amount of thrombin and the polymerization speed

In the preparation process of traditional PRP, unpolymerized fibrin is directly discarded because it dissolves in PPP. Therefore, when thrombin is added to promote blood coagulation, the content of fibrinogen has been greatly reduced, making the fibrin network structure after polymerization The density is much lower than that of physiological blood clots. Due to the action of exogenous thrombin, the high thrombin concentration makes the polymerization rate of fibrinogen much higher than the physiological reaction, and the formed fibrin network is formed by the polymerization of four molecular fibrinogen, which is stiff The lack of elasticity is not conducive to recruiting growth factors and promoting cell migration

Images