Sodium alginate/collagen composite bone scaffold as well as preparation method and application thereof

A sodium alginate and bone scaffold technology, applied in the field of regenerative medicine, can solve the problems of poor mechanical properties and unstable structure, and achieve the effects of high porosity, good biocompatibility, and easy attachment and proliferation.

Active Publication Date: 2021-08-17
SHANGHAI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, pure collagen hydrogel bone scaffolds have the problems of poor mechanical properties and unstable structure.

Method used

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  • Sodium alginate/collagen composite bone scaffold as well as preparation method and application thereof
  • Sodium alginate/collagen composite bone scaffold as well as preparation method and application thereof
  • Sodium alginate/collagen composite bone scaffold as well as preparation method and application thereof

Examples

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preparation example Construction

[0032] The invention provides a preparation method of sodium alginate / collagen composite bone scaffold, comprising the following steps:

[0033] Provide hydroxyapatite hollow microspheres loaded with antibacterial drugs;

[0034] Water, hydroxyapatite (HAP) nanoparticles, collagen cross-linking agent, collagen (Col), D-gluconolactone, sodium alginate (SA) and the hydroxyapatite hollow microparticles loaded with antibacterial drugs The balls are subjected to a pre-crosslinking reaction to obtain a pre-crosslinking reaction mixture;

[0035] Printing the pre-crosslinked reaction mixture to obtain a primary bone scaffold;

[0036] Re-crosslinking the primary bone scaffold and divalent copper ions to obtain the sodium alginate / collagen composite bone scaffold.

[0037] In the present invention, unless otherwise specified, the raw materials used in the present invention are preferably commercially available products.

[0038] The invention provides hydroxyapatite hollow microsph...

Embodiment 1

[0084] Provides hydroxyapatite hollow microspheres loaded with antibacterial drugs:

[0085] (1) The preparation of hydroxyapatite hollow microspheres comprises the following steps:

[0086] 2.5g of polyaspartic acid and 200mL of 0.1mol / L sodium hydroxide solution were mixed in the first stage to obtain solution A;

[0087] Calcium nitrate tetrahydrate solution (2.365g calcium nitrate tetrahydrate was dissolved in 100mL of deionized water) was mixed with solution A to obtain solution B;

[0088] The diammonium hydrogen phosphate solution (0.792g of diammonium hydrogen phosphate dissolved in 100mL of deionized water) was mixed with solution B in the third stage, the mixing method was constant magnetic stirring, and the time was 30min to obtain a mixed solution;

[0089] Adjust the pH value of the mixed solution to 5.0 with dilute nitric acid;

[0090] The mixed solution was transferred to a Teflon-lined stainless steel autoclave, heated at 180°C for 3h, centrifuged at 4000rpm...

Embodiment 2

[0102] Prepare sodium alginate / collagen composite bone scaffold according to the method of Example 1, the only difference is:

[0103] The concentration of amoxicillin was 10 mg / mL.

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Abstract

The invention belongs to the technical field of regenerative medicine, and particularly relates to a sodium alginate/collagen composite bone scaffold as well as a preparation method and application thereof, and the preparation method comprises the following steps: providing hydroxyapatite hollow microspheres loaded with antibacterial drugs; carrying out a pre-crosslinking reaction on water, hydroxyapatite nanoparticles, a collagen crosslinking agent, collagen, D-glucolactone, sodium alginate and the hydroxyapatite hollow microspheres loaded with the antibacterial drugs, so as to obtain a pre-crosslinked mixture; printing the pre-crosslinked mixture to obtain a primary bone scaffold; subjecting the primary bone scaffold and bivalent copper ions to a re-crosslinking reaction so as to prepare the sodium alginate/collagen composite bone scaffold. The sodium alginate/collagen composite bone scaffold obtained by using the preparation method disclosed by the invention has good mechanical properties and a relatively stable structure, and has an antibacterial effect.

Description

technical field [0001] The invention belongs to the technical field of regenerative medicine, and in particular relates to a sodium alginate / collagen composite bone scaffold and a preparation method and application thereof. Background technique [0002] Infected bone defect is a very serious complication, mostly caused by trauma, tumor resection and congenital diseases. For large-scale bone defects, there is no way for the human body to fully heal itself, and in most cases, external surgical intervention is required to return to normal. Implant-associated bone infections such as osteomyelitis frequently occur during orthopedic trauma surgery, which hinders bone repair. Therefore, infected bone defects have always been a challenging clinical problem. [0003] Currently, methods for treating bone defects include autologous bone grafts, allogeneic bone grafts, and biomaterial scaffolds. Autologous bone grafts have the disadvantages of insufficient bone supply and secondary i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/48A61L27/54A61L27/46A61L27/58A61L27/56
CPCA61L27/48A61L27/54A61L27/46A61L27/58A61L27/56A61L2430/02A61L2300/404A61L2300/622A61L2300/112C08L89/00C08L5/04
Inventor 张海光宋永腾胡庆夕王国印
Owner SHANGHAI UNIV
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