Microbial agent based on amine oxide flavobacterium and flavobacterium oryzae as well as preparation method and application of microbial agent

Abstract

The invention belongs to the field of microorganisms, and particularly relates to a microbial agent based on amine oxide flavobacterium and flavobacterium oryzae as well as a preparation method and application of the microbial agent. The microbial agent comprises mixed fermentation liquor of amine oxide flavobacterium, flavobacterium oryzae and azospirillum brasilense, the effective viable count of the three strains in the mixed fermentation liquor is not lower than 7 X 10 < 8 > CFU / mL, and the infectious microbe rate is not higher than 3%. The microbial agent can be colonized in soil for a long time, has the effects of improving soil fertility and water-retaining property, can secrete plant hormones, and regulates plant growth by promoting plant root growth.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a microbial agent based on Flavobacterium amineoxidans and Flavobacterium oryzae, a preparation method and application thereof. Background technique [0002] With the increasing application of chemical fertilizers year by year, its adverse effects on the soil have gradually become prominent. Excessive application of chemical fertilizers has caused soil degradation, soil compaction, soil overnutrition, water and soil erosion, and soil salinization, resulting in a decrease in soil moisture and water retention capacity and soil aggregate structure. Destruction, soil pH out of control, low soil nitrogen fixation capacity, etc., reduce the soil's ability to protect crops, thereby reducing the stress resistance of crops, and eventually lead to crop disease and reduced production. For example, the freezing damage that occurred in the Central China Plain in the winter of 2020 aff...

AI Technical Summary

Problems solved by technology

The prior art shows that most of the bacterial species that can effectively colonize in the rhizosphere environment are Gram-negative bacteria, so whether the active ingredients in these prior art can effectively colonize still needs to be considered. Long-term colonization of plant rhizosphere, often with low fertilizer efficiency and short duration

In addition, in the prior art such as CN103952349B, its effective active bacteria is only one strain of Bacillus arborii, and its bacterial species is difficult for Gram-positive bacteria to play a role. No matter whether it can play a role, a single strain has only one effect. Difficult to solve complex soil problems

[0006] Most of the existing agricultural microbial agent products are based on Gram-positive bacteria in the selection of strains. Because Gram-positive bacteria are difficult to colonize and play a role in the rhizosphere environment, a small amount of Gram-negative bacteria are the main species. The main microbial agent products are difficult to produce and store stably, and difficult to colonize and play a role in the field because of their single strains, low viability, and insufficient adaptability to the diversity of the field.

[0007] In addition, traditional planting techniques often rely on a large amount of chemical fertilizers, but the application of a large amount of chemical fertilizers into the field will inevitably reduce the utilization rate of fertilizers, inorganic fertilizers will be mineralized and chelated, and organic fertilizers will degrade slowly. Potential fertility lacks effective use measures, and lacks the promotion of crop rhizosphere absorption capacity

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