Biosensor based on earthworm coelomic fluid, application of biosensor in detection of tetracycline antibiotics and detection method

A tetracycline and antibiotic technology, which is applied in the field of biosensors and achieves the effects of simple extraction method, good optical performance and simple detection process

Inactive Publication Date: 2021-08-31
四川中科微纳科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, various fluorescent probes have been synthesized for the detection of tetracycline antibiotics, but there is

Method used

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  • Biosensor based on earthworm coelomic fluid, application of biosensor in detection of tetracycline antibiotics and detection method
  • Biosensor based on earthworm coelomic fluid, application of biosensor in detection of tetracycline antibiotics and detection method
  • Biosensor based on earthworm coelomic fluid, application of biosensor in detection of tetracycline antibiotics and detection method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Example 1

[0037] A method of preparation based on the biosensor of the hormone cavity, including the following steps:

[0038] (1) The stimulation liquid was prepared by mixing 10% ethanol, 0.01 mPbs buffer and 2.5 g / L ethylenediamine tetracetate (EDTA).

[0039] (2) The adult red love is wiped and wiped, placed in a petri dish containing a stimulating liquid equipped with 蚓.

[0040] (3) Collect the exhausted body cavity to the centrifugal tube, and freeze-80 ° C.

Example Embodiment

[0041] Example 2

[0042] Example 1 Extraction Extraction Body Cavity Biosensor was observed using a laser confocal microscope and flow cytometry ( Figure 1-3 ). The results showed that the rumor cavity contains two cells, wherein only the particulate cell has spontaneous fluorescence, the fluorescence intensity is proportional to the vesicle size. Under different excitation, it can emit purple, green, yellow and red fluorescence, indicating that it has excited Dependency.

Example Embodiment

[0043] Example 3

[0044] The biosensor of the humeral cavity prepared in Example 1 detected tetracycline antibiotics, including the following steps:

[0045] (1) First configure 250 μmol / L tetracycline antibiotic standard solution, diluted tetracycline antibiotics to the following concentrations by PBS buffer (pH = 7.2-7.4) and double vapor water: 0.5, 0.75, 1, 2.5, 5, 7.5, 10, 25, 50, 75 μmol / L.

[0046] (2) Remove the -80 ° C for freezing sputum volatilum, 10 min under the conditions of 10000 r / min, and then collect the upper solution as a rumor cavity liquid detection fluid.

[0047] (3) 1 ml of the above different concentrations of tetracycline antibiotic antibiotic solution were mixed with 1 ml of the body cavity to detect the fluorescence spectrum at different excitation wavelengths, and the fluorescence spectrum in the range of 450-650 nm was measured to obtain The best emission wavelength corresponding to the strongest fluorescence intensity.

[0048](4) The fluores...

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Abstract

The invention discloses a biosensor based on earthworm coelomic fluid, application of the biosensor in detection of tetracycline antibiotics and a detection method. The biosensor is derived from a coelomic fluid of eisenia foetida, wherein the coelomic fluid emits green fluorescence under excitation of 370 nm, and a fluorescence emission peak is located at 520 nm and can be quenched under the action of tetracycline antibiotics. The invention also discloses an application of the biosensor in selective detection of tetracycline antibiotics. In the detection process, along with the increase of the concentration of the added tetracycline antibiotics, the fluorescence intensity of an emission peak at 520 nm is reduced, so that the content of the tetracycline antibiotics in the sample can be detected according to the quenching degree of fluorescence. Compared with the traditional method, the method for detecting the tetracycline antibiotics provided by the invention has the advantages of high selectivity, high sensitivity, biological non-toxicity and compatibility.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to a biosensor based on body cavity fluid of earthworms, a preparation method thereof, and a method for detecting tetracycline antibiotics by the biosensor. Background technique [0002] Tetracycline antibiotics are a class of broad-spectrum antibiotics. They usually have good antibacterial and sterilizing effects on most bacteria, so they are widely used to treat various diseases. They can also be used as veterinary drugs to promote animal growth and prevent disease etc. However, in recent years, the illegal abuse of tetracycline antibiotics in the livestock and poultry breeding process has been very serious, resulting in them being flooded in animal-derived foods. In addition, in the process of livestock and poultry farming, some tetracycline antibiotics cannot be absorbed by animals, and are excreted in the form of mothers, accumulate in soil and freshwater enviro...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6428G01N2021/6417G01N2021/6432
Inventor 霍峰蒋志刘冠辉王曦妮王显祥
Owner 四川中科微纳科技有限公司
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