Cascade catalytic platform modified intraocular lens and preparation method of intraocular lens

A technology of intraocular lens and platform, applied in the direction of prosthesis, medical formula, coating, etc., can solve the problems of toxic and side effects of tissues around the eye, and achieve the effects of high intraocular biocompatibility, low cost, and simple manufacturing process

Active Publication Date: 2021-09-03

WENZHOU MEDICAL UNIV

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AI-Extracted Technical Summary

Problems solved by technology

The above-mentioned method of loading drugs on the surface of the intraocular lens has a certain preventive effect on post-cataract, b...

Abstract

The invention specifically relates to the technical field of ophthalmic medical implant materials and instrument surface modification, and in particular relates to a cascade catalytic platform modified intraocular lens and a preparation method of the intraocular lens. The surface of the intraocular lens is modified with a layer of cascade catalytic platform which takes intraocular glucose as a substrate to promote generation of endogenous active oxygen. The generated active oxygen can promote apoptosis and necrosis of residual lens epithelial cells, so that a good posterior cataract prevention and treatment effect is achieved under the condition that no toxic or side effect medicine is introduced, and the intraocular lens with high intraocular biocompatibility and the good posterior cataract prevention and treatment effect is obtained. According to the invention, a dual-enzyme-loaded cascade catalysis platform is constructed through a simple amidation reaction, and the dual-enzyme-loaded cascade catalysis platform is further modified to the surface of the intraocular lens through the action of an amido bond, so that the preparation process is simple, and the dual-enzyme-loaded cascade catalysis platform can be used for surface modification of various medical implantable devices, and is convenient to manufacture, low in cost, high in safety and convenient for industrial production.

Application Domain

Pharmaceutical delivery mechanismTissue regeneration +2

Technology Topic

Intraocular lensImplant material +15

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Examples

Experimental program(3)

Example Embodiment

[0029] Example 1

[0030] First, take a clean intraocular lens, immerse it in a 50 mg/mL polyethyleneimine aqueous solution, stir at room temperature for 12 hours, and modify the surface of the intraocular lens with amino groups, rinse with water, and dry with nitrogen for use.

[0031] Next, take 50 mg/mL mesoporous silica nanoparticles, 10 mg/mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 10 mg/mL N-hydroxyl Succinimide, 10 mg/mL glucose oxidase and 10 mg/mL horseradish peroxidase were uniformly mixed in an aqueous system, adjusted to pH 7.4, stirred at 4°C for 24 hours, washed with phosphate aqueous buffer solution for 3 Next, the cascade catalytic platform is obtained by centrifugal collection.

[0032] Finally, take 50 mg/mL cascade catalytic platform, 10 mg/mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 10 mg/mL N-hydroxysuccinimide The amine is uniformly mixed in the aqueous system, and put into the intraocular lens with surface amination, adjust the pH to 7.4, stir at 4°C for 24 hours, wash with water, and blow dry with nitrogen to obtain the intraocular lens modified by the cascade catalytic platform. .

[0033] The surface morphology of the intraocular lens modified by the cascade catalytic platform prepared in this example is as follows: figure 2 shown.

[0034] The intracellular reactive oxygen species level of the intracellular lens epithelial cells modified by the cascade catalytic platform prepared in this example after culturing for 1 hour is as follows: image 3As shown, it can be seen that the intraocular lens modified by the cascade catalytic platform prepared in this example can promote the generation of endogenous reactive oxygen species.

[0035] The apoptosis and necrosis of the lens epithelial cells on the surface of the intraocular lens modified by the cascade catalytic platform prepared in this example are as follows after 8 hours of culture. Figure 4 As shown, it can be seen that the intraocular lens modified by the cascade catalytic platform prepared in this example can promote the apoptosis and necrosis of the residual lens epithelial cells.

[0036] The effects of cell proliferation and adhesion on the lens epithelial cells on the surface of the intraocular lens modified by the cascade catalytic platform prepared in this example are as follows after 72 hours of culture. Figure 5 As shown, it can be seen that the intraocular lens modified by the cascade catalytic platform prepared in this example can effectively inhibit the proliferation and adhesion of residual lens epithelial cells.

Example Embodiment

[0037] Example 2

[0038] First, take a clean intraocular lens, immerse it in a 0.3 mg/mL polyethyleneimine aqueous solution, and stir at room temperature for 24 hours to modify the surface of the intraocular lens with amino groups, rinse with water, and dry with nitrogen for use.

[0039] Next, take 0.5 mg/mL protein scaffold, 0.1 mg/mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 0.1 mg/mL N-hydroxysuccinimide and 0.01 mg/mL gold nanoparticles and 0.01 mg/mL hemoglobin were uniformly mixed in the aqueous system, adjusted to pH 7.2, stirred at 4°C for 12 hours, washed with phosphate aqueous buffer solution three times, and collected by centrifugation to obtain the cascade Catalytic platform.

[0040] Finally, take 0.5 mg/mL cascade catalytic platform, 0.1 mg/mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 0.1 mg/mL N-hydroxysuccinimide The amine was uniformly mixed in the aqueous system, and put into the intraocular lens with surface amination, adjusted to pH 7.2, stirred at 4°C for 12 hours, washed with water, and blown dry with nitrogen to obtain the intraocular lens modified by the cascade catalytic platform. .

Example Embodiment

[0041] Example 3

[0042] First, take a clean intraocular lens, immerse it in a 20 mg/mL polyethyleneimine aqueous solution, stir at room temperature for 36 hours, so that the surface of the intraocular lens is modified with amino groups, rinse with water, and dry with nitrogen for use.

[0043] Next, take 20 mg/mL polymersomes, 5 mg/mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 5 mg/mL N-hydroxysuccinimide Amine, 1 mg/mL graphitic carbon nitride nanomaterials and 1 mg/mL ceria nanoparticles were uniformly mixed in an aqueous solution, adjusted to pH 7.0, stirred at 4 °C for 6 hours, and washed with a phosphate aqueous buffer solution for 3 Next, the cascade catalytic platform is obtained by centrifugal collection.

[0044] Finally, take 20 mg/mL cascade catalytic platform, 5 mg/mL 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 5 mg/mL N-hydroxysuccinimide The amine was uniformly mixed in the aqueous system, and put into the intraocular lens with surface amination, adjusted to pH 7.0, stirred for 6 hours at 4°C, washed with water, and blown dry with nitrogen to obtain the intraocular lens modified by the cascade catalytic platform. .

[0045] The principle of the present invention is that high levels of intracellular reactive oxygen species are harmful to cells, which can induce apoptosis or necrosis by triggering oxidative damage to proteins, nucleic acids, mitochondria and cell membranes. Co-loading multiple enzymes to form a cascade catalytic reaction system through appropriate spatial regulation is one of the common strategies to simulate the generation of reactive oxygen species in nature. In the invention, the substance with glucose oxidase function and the material with peroxidase function are co-fixed in the pores of the micro/nano reactor through amidation reaction to form a cascade catalytic reaction platform with dual enzymes jointly loaded. The cascade catalytic platform is further modified on the surface of the intraocular lens by the action of amide bonds, and the intraocular lens modified by the cascade catalytic platform is obtained.

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Cascade catalytic platform modified intraocular lens and preparation method of intraocular lens

A technology of intraocular lens and platform, applied in the direction of prosthesis, medical formula, coating, etc., can solve the problems of toxic and side effects of tissues around the eye, and achieve the effects of high intraocular biocompatibility, low cost, and simple manufacturing process

AI-Extracted Technical Summary

Problems solved by technology

Abstract

Image

Examples

Example Embodiment

Example Embodiment

Example Embodiment

PUM

Description & Claims & Application Information

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