Physical stimulation method for increasing yield of penicillin

Abstract

The invention discloses a physical stimulation method for increasing the yield of penicillin. The method comprises the following steps of 1) preparing atmospheric pressure low-temperature plasma activated water PAW, and further forming an atmospheric pressure low-temperature plasma activated solid culture medium PASM and atmospheric pressure low-temperature plasma activated microbubbles PAB; 2) inoculating penicillium chrysogenum suspension obtained through soil separation into PASM, and putting the materials into an incubator to be cultured; 3) preparing a penicillium chrysogenum spore solution from PASM cultured in step 2), adding PAB, and performing stimulation treatment by using a nanosecond pulse power technology; and 4) adding the PAW prepared in the step 1) into a culture medium containing the penicillium chrysogenum spore solution treated in the step 3) in a dropwise adding manner on the basis of an automatic dosage control method, and performing fermentation so that the yield of penicillin is increased. According to the method, the plasma technology and the nanosecond pulsed electric field technology are adopted to intervene the penicillium chrysogenum, the expression of pcbAB, pcbC and penDE is effectively up-regulated, meanwhile, the growth cycle of the penicillium chrysogenum is obviously shortened, and the yield of penicillin is greatly increased.

Description

Technical field [0001] The present invention relates to a method of improving penicillin production, belonging to the field of biotechnology. Background technique [0002] Penicillin is a kind of natural antibiotics produced by yellowish artica, with high antibacterial, high efficacy, low toxicity, is included in the basic drug standard list of the World Health Organization, is the most effective and safe and safest of human beings. One of the basic health drugs. At present, the main techniques for improving penicillin fermentation and shortening the fermentation cycle include: Mutagenicity Breeding of high strains; gene engineering orientation acquisition high strains; optimized strains fermentation environmental conditions, and increase the fermentation of strains. However, existing technical methods have many inherent disadvantages in practical applications. The problem of mutagenic breeding is that the forward mutation caused by the excessive genetic load and the increase of ...

AI Technical Summary

Problems solved by technology

However, the existing technical means have many inherent shortcomings in practical application.

The problem of mutagenesis breeding is that the positive mutations caused by the overload of genetic load and the increase of drug resistance of the strains decrease or even disappear, which makes it difficult to breed new high-yielding strains

The problem with genetic engineering is that it relies heavily on effective gene targets. Due to the limitation of the number of gene targets, it is increasingly difficult to construct mature and stable engineered bacteria.

The problem with optimizing the fermentation environment conditions of strains lies in the existence of the upper limit of optimization. For a certain strain, when the external factors reach the optimal combination, it will not be possible to continue to optimize to further increase the fermentation yield and shorten the fermentation cycle.

For Penicillium chrysogenum, which has been studied for a long time to increase production, the above problems are particularly prominent, and have become a major bottleneck restricting the development of penicillin industry

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