Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fluorescent probe based on benzothiazole fluorophore and application of fluorescent probe in hypochlorous acid detection

A fluorescent probe, benzothiazole technology, applied in the field of fluorescent probe based on benzothiazole fluorophore and in the detection of hypochlorous acid, achieving obvious results

Active Publication Date: 2021-10-01
GUANGDONG UNIV OF PETROCHEMICAL TECH
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few reports on the fluorescence properties of HBT and its methyl derivatives in different solvents

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent probe based on benzothiazole fluorophore and application of fluorescent probe in hypochlorous acid detection
  • Fluorescent probe based on benzothiazole fluorophore and application of fluorescent probe in hypochlorous acid detection
  • Fluorescent probe based on benzothiazole fluorophore and application of fluorescent probe in hypochlorous acid detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Fluorescent probe preparation:

[0039] Add 3mmol of salicylaldehyde and 3.2mmol of 2-aminomercaptophenol into a round bottom flask filled with 6mL of ethanol solution, and connect a glass three-way valve with a balloon attached to the flask. Vacuumize the flask and pass nitrogen through a glass three-way valve with a balloon, and react at room temperature for 30 minutes. After reacting for 30 min, quickly add 0.34 mL of H 2 o 2 (30%, mass) and 0.17 mL of HCl (37%, mass). Continue to react under nitrogen atmosphere for 12h.

[0040] After the reaction was over, the flask was kept under ice-water conditions with 0.05 mL of HCl (37%), then the solution was transferred to a 100 mL beaker, and 30 mL of saturated saline was added. Extracted 3 times with 50 mL of ethyl acetate, and evaporated the sample to dryness by a rotary evaporator to obtain a preliminary purified sample.

[0041] The preliminarily purified sample was dissolved in ethyl acetate and an appropriate am...

Embodiment 2

[0043] 0.01 mmol of the probe was dissolved in 10 mL of methanol solution to obtain a 1 mM probe stock solution. Take 20 μL of the probe stock solution and add it to the cuvette of methanol and aqueous solution, then gradually add 5, 10, 15, 20 and 25 μL of 0.04M sodium hydroxide solution. When it was added to 20 μL, the fluorescence intensity basically stopped increasing. Therefore, 20 μL of NaOH solution with a concentration of 0.04 M was added under the same conditions for subsequent solvent environments. And at the same time, the corresponding ultraviolet absorption spectrum is measured and added. Such as figure 1 , figure 2 shown.

Embodiment 3

[0045] Take 20 μL of the probe stock solution described in Example 2 and add it to the centrifuge tubes of water, methanol, ethanol, isopropanol, N,N-dimethylformamide, acetonitrile, dimethyl sulfoxide and acetone solvents respectively , and then add 20 μL of NaOH solution with a concentration of 0.04M, and then add the same and excess sodium hypochlorite solution, and take fluorescence pictures under a UV lamp with a wavelength of 365 nm. It was found that in methanol solution, the quenching effect of the probe was the best (obvious fluorescence quenching). Such as image 3 .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention aims to provide a fluorescent probe based on benzothiazole fluorophore and application of the fluorescent probe in hypochlorous acid detection. The fluorescent probe disclosed by the invention is a fluorescent probe for visually detecting pypocholoride. The fluorescent probe is an HBT methyl derivative. The invention verifies the fluorescence stability in different solvents, and is applied to linear detection of hypochlorous acid / pypocholoride based on the fluorescence stability. The probe disclosed by the invention can be prepared into a test paper sensor for quickly, visually and semi-quantitatively detecting the concentration of hypochlorous acid / pypocholoride in a solution on site.

Description

technical field [0001] The invention belongs to the technical field of fluorescence analysis and detection of hypochlorous acid and hypochlorite, and in particular relates to a fluorescent probe based on a benzothiazole fluorophore and its application in the detection of hypochlorous acid. Background technique [0002] Hypochlorite, especially sodium hypochlorite, is widely used in various environmental disinfections in daily life because of its strong oxidizing ability. Sodium hypochlorite is widely used for indoor and outdoor disinfection during the COVID-19 pandemic. In some cities with severe epidemics, in order to completely eliminate the remaining viruses in the environment, the epidemic prevention departments use a large amount of sodium hypochlorite to eliminate potential viruses in every corner of the city. While the virus was being wiped out, the sodium hypochlorite left in the environment also entered rivers and lakes through sewers. However, excess sodium hypoc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D277/66C09K11/06G01N21/64
CPCC07D277/66C09K11/06G01N21/643C09K2211/1037G01N2021/6432
Inventor 王素华余龙李鑫孙明泰苏鹏辰殷冉皓
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com