A kind of fluorescent probe molecule and its preparation method and application

A technology of fluorescent probes and molecules, which is applied in the field of fluorescent probe molecules and their preparation, can solve the problems of lack of detection and achieve the effects of simple steps, high sensitivity and good selectivity
CN113501818BActive Publication Date: 2022-06-07YUNNAN NORMAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YUNNAN NORMAL UNIV
Publication Date
2022-06-07

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Abstract

The invention provides a fluorescent probe molecule, relates to the technical field of organic functional materials, and is used for simultaneously monitoring hypochlorous acid and pH; a fluorescent probe molecule is a methylene blue-4-aminonaphthalimide derivative, which has the The amide group that reacts with acid, and the o-phenylenediamine group that responds to the pH value; HClO has a strong oxidizing property, which can break the amide bond in the fluorescent probe molecule, generate an unstable intermediate, and finally release A positively charged methylene blue fluorophore is produced, and fluorescence (690nm) appears in the near-infrared region; when the proton is combined with the 4-position o-phenylenediamine, the PET effect is suppressed, resulting in yellow-green fluorescence (525nm), thereby achieving double recognition Site selective recognition and simultaneous detection of hypochlorous acid and pH value; the invention also provides a preparation method and application of a fluorescent probe molecule; the fluorescent probe molecule is used for detecting hypochlorous acid and pH in multiple cells.
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Description

technical field

[0001] The invention relates to the technical field of organic functional materials, and more particularly, to a fluorescent probe molecule and a preparation method and application thereof. Background technique

[0002] Optical probe molecular imaging technology has good application prospects in the field of surgery and biomedicine. In recent years, fluorescence imaging technology has achieved rapid development. Due to its convenient, non-invasive, real-time in-situ dynamic visual monitoring of biological samples, high sensitivity and high temporal and spatial resolution, it has gradually become an important research field of biological fluorescent probes. tool.

[0003] Fluorescent probes for the detection of biologically active small molecules (reactive oxygen species (ROS), reactive nitrogen species (RNS), reactive sulfur (RSS), etc.) and biological in vivo fluorescence imaging research are current research hotspots. Compared with single detection fluore...

Claims

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