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Application of stem cell-derived exosome in preparation of medicine for treating chronic obstructive pulmonary disease

A chronic obstructive pulmonary and exosome technology, applied in the field of lung disease treatment, can solve the problems of inability to guarantee the effect of colonization treatment, short survival time of stem cells, and side effects that cannot be ignored, so as to inhibit inflammation, prevent immune rejection, and improve lung function Effect

Pending Publication Date: 2021-10-22
THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the deepening of stem cell research, the problems brought about by its application have gradually become prominent
The application of stem cells has its limitations. After transplantation, the survival time of stem cells in the body is short, and the therapeutic effect of colonization after transplantation cannot be guaranteed.
In addition, we cannot ignore its unavoidable side effects, especially tumorigenicity and immunogenicity

Method used

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  • Application of stem cell-derived exosome in preparation of medicine for treating chronic obstructive pulmonary disease
  • Application of stem cell-derived exosome in preparation of medicine for treating chronic obstructive pulmonary disease
  • Application of stem cell-derived exosome in preparation of medicine for treating chronic obstructive pulmonary disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1. Preparation and expansion of mesenchymal stem cells

[0063] The obtained P0 mesenchymal stem cells were isolated, and the original cell bank and the working cell bank were established in the P3, P5 and P6 generations, respectively. Cell phenotype detection and identification of P6 generation cells. After the cell density is greater than 70%, the medium is replaced, and the cell culture supernatant of the P5-P6 generation cell expansion culture is collected after 48 hours.

[0064] 2. Extraction and identification of exosomes

[0065] Centrifuge the collected culture supernatant A mL at 3000g for 30min at 4°C to remove dead cells, take the supernatant and continue to centrifuge at 10000g for 30min to remove cell debris, transfer the supernatant to an ultrafiltration tube and concentrate to B mL (A:B approx. is 20:1). Use an ultracentrifuge to separate and further purify exosomes, and centrifuge twice at 110,000 g for 70 min. The precipitate was resuspended in 100...

Embodiment 2

[0074] 1. Efficacy evaluation of stem cell-derived exosomes in the treatment of CODP model mice

[0075] (1) Construction of COPD model mice and drug administration design

[0076] After passing the quarantine, C57BL / 6J mice were randomly divided into normal control group (CTL), model group, MSCs group, and MSCs-derived exosomes group. Using smoke exposure for 6 months, establish a mouse COPD animal model, specifically: 4-6 weeks old mice, after a week of adaptation, use domestic cheap cigarettes, conduct whole-body exposure in a smoke box, and give mice passive smoking , exposed to cigarette smoke 6 days a week for 6 months. Cell therapy was started after 3 months of smoke exposure, administered once a month at a dose of 200 μg / 200 μl / mouse, and treated for 3 times in total, and samples were taken to evaluate the therapeutic effect after 6 months of smoke exposure.

[0077] (2) Evaluation of the potency of exosomes in the treatment of COPD

[0078] In the model group, the ...

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Abstract

The invention relates to the technical field of lung disease treatment, in particular to application of stem cell-derived exosomes in preparation of drugs for treating chronic obstructive pulmonary diseases. The research shows that the exosome which is small in structure and can be more easily taken by recipient cells has a treatment effect similar to that of stem cells in treatment of chronic obstructive pulmonary diseases, and is more easily taken by the recipient cells due to no immunological rejection, so that the exosome can be used as a substitute of the stem cells and can be used for treating chronic obstructive pulmonary diseases. A new thought and strategy are provided for treating the chronic obstructive pulmonary disease.

Description

technical field [0001] The present invention relates to the technical field of lung disease treatment, in particular to the application of exosomes derived from stem cells in the preparation of drugs for the treatment of chronic obstructive pulmonary disease. Background technique [0002] Chronic obstructive pulmonary disease, characterized by persistent respiratory symptoms and airflow limitation, is a common preventable and treatable chronic respiratory disease. Smoking is one of the main causes of COPD, and its pathogenesis is very complex. In addition to lung transplantation, there is still no effective cure for COPD clinically. [0003] For the pathogenesis of COPD, stem cell transplantation and the application of cytokines and other biotherapeutic methods have become the treatment methods for COPD because there is no transplant rejection, and they can be enriched to the damaged site to exert anti-inflammatory, immunosuppressive, and anti-apoptotic effects. One of the...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K9/00A61K9/12A61P11/00C12N5/0775
CPCA61K35/28A61K9/0019A61K9/12A61K9/0043A61P11/00C12N5/0662C12N2509/00
Inventor 孙瑞婷冉丕鑫吴祖泽周玉民杨月峰王华白鸽孔攀月
Owner THE FIRST AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV (GUANGZHOU RESPIRATORY CENT)
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