Supercharge Your Innovation With Domain-Expert AI Agents!

Application of bacteroides cellulosilyticus in prevention and/or treatment of inflammatory bowel diseases

A technology of inflammatory bowel disease and cellulose, applied in the field of microorganisms, can solve the problems that the application has not been disclosed, and achieve the effect of great application value, no toxic side effects, and excellent resistance

Pending Publication Date: 2021-10-22
GUANGZHOU ZHIYI PHARMA INC
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the bacterium itself and its use in food have been published, its use in the prevention and treatment of IBD has not been published

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of bacteroides cellulosilyticus in prevention and/or treatment of inflammatory bowel diseases
  • Application of bacteroides cellulosilyticus in prevention and/or treatment of inflammatory bowel diseases
  • Application of bacteroides cellulosilyticus in prevention and/or treatment of inflammatory bowel diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 The cultivation of Bacteroides cellulolyticum

[0055] Streak activated strain: DSM 14838T strain of Bacteroides cellulolyticum, used BHA (brain heart infusion agar medium) plate for streak activation. Anaerobic culture at 37°C for 2-7 days to obtain a single colony.

[0056] Colony characteristics: After Bacteroides cellulolyticum was cultured on the BHA plate for 48 hours, the colony characteristics were round, slightly convex, translucent, white, and smooth.

[0057] Morphology under the microscope: Bacteroides cellulolyticum was subjected to Gram-staining microscopic examination, and it was a Gram-negative bacterium with a typical rod shape, blunt rounded ends and dense staining, and the uncolored part in the middle of the bacteria was like a vacuole. figure 1 .

[0058] Sludge preparation: select a single colony and inoculate it in tryptone broth for 8 hours of fermentation (at a temperature of 37°C), centrifuge the obtained bacterial solution at a spee...

Embodiment 2

[0059] Example 2 The efficacy experiment of Bacteroides cellulolyticum in the treatment of Crohn's disease in mice induced by trinitrobenzenesulfonic acid (TNBS) combined with ethanol

[0060] experimental design

[0061] 60 SPF healthy BALB / c mice, female, average weight 18-22g, average age 6-8 weeks. After 1 week of adaptive feeding, they were randomly divided into groups as follows: blank group, model group, mesalazine group, low-, medium-, and high-dose groups of Bacteroides cellulolyticum (BC).

[0062] Modeling: In addition to the blank group, 2.0 mL of 3% chloral hydrate was injected intraperitoneally once a week, and after anesthesia, 0.1 mL of 2.0 mg TNBS / 50% ethanol mixed enema solution was administered as an enema using a 12 mm size mouse enema. The blank group was given 0.1 mL of normal saline by enema once a week. Administration for 6 consecutive weeks.

[0063] Dosing: After 6 weeks of modeling, except for the model group, the mice in each group were given the...

Embodiment 3

[0078] Example 3 Drug efficacy experiment of Bacteroides cellulolyticum in treating chronic ulcerative colitis in rats induced by dextran sulfate sodium salt (DSS)

[0079] experimental design:

[0080] Select 60 healthy and mature SD rats, half male and half female, body weight 200±20g, purchased from Guangdong Experimental Animal Center, raised in SPF grade animal room, randomly divided into 6 groups, 10 rats in each group, all rats before experiment Adapt to the environment for 1 week, and feed normal rat chow.

[0081] Using 5% dextran sulfate sodium salt (dextran sulfate sodium, DSS), purchased from Sigma, USA) solution to induce chronic ulcerative colitis model in rats, 60 SD rats were randomly divided into the following 6 groups:

[0082] Normal control group, model group, mesalazine group, BC low-dose group, BC medium-dose group, BC high-dose group, DAI score and tissue injury score were used to detect the curative effect of each intervention group. The animal groupi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of bacteroides cellulosilyticus in prevention and / or treatment of inflammatory bowel diseases, in particular to application of bacteroides cellulosilyticus in preparing medicines, pharmaceutical compositions, food, health-care products, food additives and the like for preventing and / or treating the inflammatory bowel diseases. Results of related in-vivo and in-vitro experiments prove that the bacteroides cellulosilyticus has excellent resistance to inflammatory bowel diseases, including ulcerative colitis and Crohn's disease, and has no toxic or side effect; and the bacteroides cellulosilyticus has durable and effective effect when applied to preparation of the medicines, the pharmaceutical compositions, the food, the health care products or the food additives and the like for preventing and / or treating the inflammatory bowel diseases. The medicines, the pharmaceutical compositions, the food, the health care products or the food additives can be used for preventing and treating the inflammatory bowel diseases and have great application value.

Description

technical field [0001] The present invention relates to microbial technology, especially the application of Bacteroides cellulolyticum in the prevention and / or treatment of inflammatory bowel disease, including the preparation of medicines, pharmaceutical compositions, food and health care for the prevention and / or treatment of inflammatory bowel disease products and food additives. Background technique [0002] Inflammatory bowel disease (Inflammatory bowel disease, IBD) is a group of chronic intestinal inflammatory diseases of unknown cause, including ulcerative colitis (Ulcerative colitis, UC) and Crohn's disease (Crohn's disease, CD). The former, also known as nonspecific ulcerative colitis, is an inflammation of the rectum and colon of unknown cause, and the lesions are mainly limited to the mucosa and submucosa of the large intestine. The latter is a kind of chronic granulomatous inflammation, and the lesions can involve various parts of the gastrointestinal tract, ma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/74A61P1/00A61P1/04A23L33/135A23L29/00
CPCA61K35/74A61P1/00A61P1/04A23L33/135A23L29/065A23V2002/00A23V2200/32
Inventor 刘洋洋郑丽君常秀娟梁德宝林渝清
Owner GUANGZHOU ZHIYI PHARMA INC
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com