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A 3D printing drug-loaded bone defect filler scaffold and its preparation method and application

A 3D printing and filler technology, used in 3D printing, drug delivery, bone implants, etc., can solve the problems of non-absorbable metabolism of stabilizers and auxiliaries, the proportion of active ingredients is reduced, and there is no sustained release effect. The effect of inhibiting bacterial or tumor cell growth, promoting osteogenesis in vivo, and facilitating regulation of release

Active Publication Date: 2022-05-24
SHANGHAI BALCK FLAME MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a certain amount of stabilizers and auxiliaries need to be added to the slurry during printing, resulting in a decrease in the proportion of active ingredients (generally inorganic materials) in the final product, and the stabilizers and auxiliaries are usually not absorbed and metabolized by the human body; soaking the drug solution The way of drug loading is easy to release the drug too fast, without sustained release effect

Method used

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  • A 3D printing drug-loaded bone defect filler scaffold and its preparation method and application
  • A 3D printing drug-loaded bone defect filler scaffold and its preparation method and application
  • A 3D printing drug-loaded bone defect filler scaffold and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0067] A preparation method of a 3D printed drug-loaded bone defect filler scaffold is as follows:

[0068] (1) After drying the tricalcium phosphate (β-TCP) powder and the hydroxyapatite (HA) powder, under vacuum at 60°C overnight, use a 500-mesh stainless steel screen to sieve the two powders respectively. The two powders are reserved;

[0069] (2) Fully dissolve 0.8g of L-polylactic acid (PLLA) in 3.5mL of dichloromethane, then slowly add 1.2g of sieved tricalcium phosphate (β-TCP) and 2g of hydroxyapatite (HA) powder, Then 1 mL of absolute ethanol was added, and the matrix slurry was obtained by fully stirring at 1500 rpm for 0.5 h;

[0070] (3) Dissolve 450 mg of dopamine in 6 mL of ultrapure water, fully dissolve to obtain solution A, fully mix 81 mL of ethanol, 36 mL of ultrapure water and 2.7 mL of ammonia to obtain solution B, add A to solution B to react for 24 hours, and then After centrifugation to remove the supernatant, freeze-dried to obtain polydopamine (PDA)...

Embodiment 2

[0080] A preparation method of a 3D printed drug-loaded bone defect filler scaffold is as follows:

[0081] (1) After drying the tricalcium phosphate (β-TCP) powder and the hydroxyapatite (HA) powder, under vacuum at 60°C overnight, use a 500-mesh stainless steel screen to sieve the two powders respectively. The two powders are reserved;

[0082] (2) Fully dissolve 1.44g L-polylactic acid (PLLA) in 5.4mL dichloromethane, then slowly add sieved 2.88g tricalcium phosphate (β-TCP) and 2.88g hydroxyapatite (HA) powder , and then add 1.8 mL of anhydrous ethanol, and fully stir at 1500 rpm for 1 h to obtain a matrix slurry;

[0083] (3) Dissolve 450 mg of dopamine in 6 mL of ultrapure water, fully dissolve to obtain solution A, fully mix 81 mL of ethanol, 36 mL of ultrapure water and 2.7 mL of ammonia to obtain solution B, add A to solution B to react for 24 hours, and then The supernatant was removed by centrifugation and then freeze-dried to obtain 200 nm polydopamine (PDA) micr...

Embodiment 3

[0092] A preparation method of a 3D printed drug-loaded bone defect filler scaffold is as follows:

[0093] (1) After drying the tricalcium phosphate (β-TCP) powder and the hydroxyapatite (HA) powder, under vacuum at 60°C overnight, use a 500-mesh stainless steel screen to sieve the two powders respectively. The two powders are reserved;

[0094] (2) Fully dissolve 1 g of L-polylactic acid (PLLA) in 4 mL of dichloromethane, then slowly add sieved 1.5 g of tricalcium phosphate (β-TCP) and 2.5 g of hydroxyapatite (HA) powder, and then Add 1 mL of absolute ethanol, and fully stir at 1200 rpm for 1 h to obtain a matrix slurry;

[0095] (3) Dissolve 450 mg of dopamine in 6 mL of ultrapure water, fully dissolve to obtain solution A, fully mix 81 mL of ethanol, 36 mL of ultrapure water and 2.7 mL of ammonia to obtain solution B, add A to solution B to react for 24 hours, and then The supernatant was removed by centrifugation and then freeze-dried to obtain 200 nm polydopamine (PDA)...

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Abstract

The invention provides a 3D printing drug-loaded bone defect filler scaffold, a preparation method and application thereof. By self-polymerizing dopamine under alkaline conditions in advance, polydopamine microspheres are obtained after freeze-drying, and then mixed with a matrix slurry composed of tricalcium phosphate, hydroxyapatite and L-polylactic acid to obtain a mixed slurry, which is 3D printed technology to obtain bone defect filler scaffolds with a porous structure, and then soak the scaffolds in growth factors or drug solutions for drug loading, or mix polydopamine microspheres with drugs first and then mix them with matrix slurry, and then perform 3D printing to shape the scaffolds . The drug-loaded bone defect filler scaffold obtained by the above method has the characteristics of high drug loading, good controlled release effect, and high active ingredients, and is of great significance in inhibiting bone tumors and promoting bone defect repair.

Description

technical field [0001] The present application relates to the technical field of bone repair materials, in particular, to a preparation method of a 3D printing drug-loaded bone defect filler. Background technique [0002] Bone defects caused by bone trauma, bone tumor, and bone infection are very common. When the self-healing of bone alone cannot heal, it is necessary to use implant materials such as bone repair scaffolds to assist in the repair and recovery of damaged tissue. An ideal bone repair scaffold needs to have good biocompatibility, biodegradability, three-dimensional porous structure and complex shape matching the defect site. Porous bone repair scaffolds have high specific surface area and space, which is conducive to the loading of active factors, cell adhesion growth, extracellular matrix deposition, nutrient and oxygen entry, metabolite discharge, and blood vessel ingrowth. In addition, since the scaffold needs to provide support for the new tissue until the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/46A61L27/48A61L27/22A61L27/54A61L27/56A61F2/30A61F2/28B33Y10/00B33Y70/10B33Y80/00
CPCA61L27/46A61L27/48A61L27/227A61L27/54A61L27/56A61F2/28A61F2/30942B33Y80/00B33Y70/10B33Y10/00A61F2002/2835A61F2002/30985A61L2430/02A61L2300/622A61L2300/414C08L67/04C08L79/04
Inventor 陆益栋邢天龙蒋宇奇
Owner SHANGHAI BALCK FLAME MEDICAL TECH CO LTD
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