A kind of veterinary drug against Escherichia coli diarrhea and its preparation method
A technology for Escherichia coli and diarrhea, which is applied in the field of anti-Escherichia coli diarrhea disease Mongolian veterinary medicine and its preparation, can solve the problems of destroying the body's homeostasis and excessive three roots, so as to reduce the incidence rate, improve antibacterial, and improve the survival rate of young animals and growth rate effects
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Embodiment 1
[0049] Example 1: Optimal screening experiment of Mongolian veterinary medicine BBS traditional compound
[0050] 1.1 Experimental consumables and equipment
[0051] 1.1.1 Main reagents
[0052] Common nutrient agar, eosin methylene blue nutrient agar, nutrient broth, ciprofloxacin (batch number: 11220023), purchased from Guangzhou Baiyunshan Pharmaceutical Co., Ltd.; Myrobalan, madder, black leaf, thorn bean, black Yunxiang, Rhubarb, Gansong, Qumai, etc. were purchased from Inner Mongolia Tianli Pharmaceutical Co., Ltd.
[0053] 1.1.2 Bacteria
[0054] Pathogenic Escherichia coli (E.coli O 1) was presented by Prof. Ao Rigele from the Cattle Production Laboratory, School of Animal Science, Inner Mongolia Agricultural University. The strain was isolated, identified and preserved by Dr. Yang Siqin in the experimental team of Prof. Aorigele.
[0055] 1.1.3 Experimental animals
[0056] SPF grade Kunming mice, half male and half female, weighing 23±2 g, were purchased from th...
Embodiment 2
[0127] Example 2: Clinical cure experiment of Mongolian veterinary drug BBS on calves with Escherichia coli diarrhea
[0128] 2.1 Experimental materials
[0129] 2.1.1 Experimental animals
[0130] 48 Holstein calves of similar body weight (40±2kg) were selected. They were randomly divided into 6 groups with 8 heads in each group.
[0131] 2.1.2 Test drug
[0132] 7. The ingredients were pulverized with a 200-mesh sieve, and were mixed with a 200-mesh sieve. They were all purchased from Inner Mongolia Tianli Pharmaceutical Co., Ltd. Ciprofloxacin group (production batch number: 20180316) was purchased from Chengdu Xinheng Pharmaceutical Co., Ltd.
[0133] 2.2 Test method
[0134] 2.2.1 Experimental Design
[0135] The trial was carried out at the Knight Ranch of Dalat Banner, Erdos, Inner Mongolia, with the permission of the company's owner. All experimental protocols have been approved by the Livestock Care Committee of Inner Mongolia Agricultural University and were p...
Embodiment 3
[0162] Example 3 The effect of Mongolian veterinary medicine BBS compound on intestinal mucosal biological barrier in mice with Escherichia coli diarrhea
[0163] 3.1 Experimental materials
[0164] 3.1.1 Experimental bacteria
[0165] Pathogenic Escherichia coli (E.coli O 1 ) was presented by Prof. Ao Rigele, Cattle Production Laboratory, School of Animal Science, Inner Mongolia Agricultural University, and was isolated, identified and preserved by Dr. Yang Siqin.
[0166] 3.1.2 Experimental animals
[0167] SPF grade Kunming mice, half male and half female, weighing 23±2 g, were purchased from the Experimental Animal Center of Inner Mongolia University, license number: SCXK (Mongolia 2016-0001). In a separate dry and ventilated cage, 10 per cage, constant temperature (21±2°C), constant humidity (53±5%), free access to food and water, and a light / dark cycle of 12h. Adapt to feeding for 3d and carry out experiments. The experiments were performed in accordance with the pr...
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