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Artificial periosteum compounded with acellular periosteum matrix as well as preparation method and application of artificial periosteum

A kind of acellular bone, artificial bone technology, applied in prosthesis, tissue regeneration, medical science and other directions, can solve the problems of hemolysis and coagulation, low biocompatibility, Young's modulus mismatch, etc., to achieve good mechanical properties, Good water retention performance and the effect of promoting the formation of

Active Publication Date: 2021-11-02
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Aiming at the above-mentioned deficiencies in the prior art, the object of the present invention is to provide a kind of artificial periosteum with composite decellularized periosteum matrix and its preparation method and application, so as to solve the problem of the Young's modulus mismatch between the existing artificial periosteum and the natural periosteum, the biological phase Low capacity, and problems such as hemolysis and coagulation that implant materials can cause

Method used

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  • Artificial periosteum compounded with acellular periosteum matrix as well as preparation method and application of artificial periosteum
  • Artificial periosteum compounded with acellular periosteum matrix as well as preparation method and application of artificial periosteum
  • Artificial periosteum compounded with acellular periosteum matrix as well as preparation method and application of artificial periosteum

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Embodiment 1

[0029] A composite matrix acellular artificial periosteum periosteum, using the following process:

[0030] 1) Select the level cocoon, crushing washing, by 1g: 25mL was added sodium carbonate (0.4% g / mL) boiling 2.5h, after boiling washed three times with boiling water, and then drained with a suction fibroin samples machine , placed in a drying oven 37 [deg.] C drying. Formulation chloride / absolute ethanol / water (molar ratio 1: 2: 8) mixed salt solution, 80 ℃ water bath for 30min, to fibroin dissolved. Filtering the supernatant, the solution was poured fibroin dialysis bag (molecular weight 8000-14000), and dialyzed with double distilled water three days, changed once every 12h double distilled water. After completion of the dialysis, the supernatant was centrifuged, to obtain silk fibroin solution. Freeze-dried and stored at 4 ℃ RESERVE.

[0031]2) whichever periosteum from healthy rats, which is then bathed in a concentration of 3% (m / v) solution of Triton X-100 (a 0.2...

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Abstract

The invention discloses an artificial periosteum compounded with an acellular periosteum matrix as well as a preparation method and application of the artificial periosteum. The polyvinyl alcohol / sodium carboxymethyl cellulose is adopted as a gel substrate material of the artificial periosteum, and silk fibroin and acellular periosteum matrix hydrogel are added to form a gel film material with a uniform structure. The artificial periosteum prepared by the invention is close to the physical and biological characteristics of a natural periosteum, has better biocompatibility, provides a highly hydrated 3D network structure similar to an extracellular matrix and a rough surface for cells, is beneficial to the adhesion and growth of the cells, is beneficial to the proliferation and differentiation of osteoblasts and osteogenic cells. The artificial periosteum can be used for further promoting the formation of new bones, has similar performances to periosteum in mechanics, also has good anti-hemolysis and anti-coagulation effects, is a very potential ideal material for repairing soft tissues, and has a very wide application prospect in the field of bone tissue engineering.

Description

Technical field [0001] The present invention relates to the field of tissue engineering materials, and particularly relates to an artificial periosteum preparation method and application Acellular matrix periosteum. Background technique [0002] Bone defect is a common disease in clinical orthopedics, trauma can be caused by many factors such as infections and cancer, is one of the problems of orthopedic treatment. Current research in this area focused on the more improvement of the bone replacement material, selection and development of good seed cells corresponding composite technology. Is commonly used in clinical treatment of bone grafts, i.e., the use of bone to fill bone defects scaffold and induce bone regeneration. However, after stent implantation bone, which repair process is essentially a passive connection and filling process, the lack of environmental active perceivable defects. Although the portion of the bracket combined with exogenous cytokines can play a role in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/20A61L27/16A61L27/22A61L27/52A61L27/50
CPCA61L27/3633A61L27/20A61L27/16A61L27/227A61L27/52A61L27/50A61L2430/02C08L1/286C08L29/04C08L89/00
Inventor 陈国宝陆唐芳董刚立王靳松夏斌王富平陈忠敏
Owner CHONGQING UNIV OF TECH
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