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Preparation method of porous alloy nano material and application of porous alloy nano material in plasma metabolite detection

A technology of alloy nanomaterials and metabolites, which is applied in the field of porous alloy nanomaterial synthesis to achieve the effect of solving hot spot effect, low cost and high detection throughput

Pending Publication Date: 2021-11-02
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the above-mentioned defects of the prior art, the technical problem to be solved by the present invention is how to have a MALDI MS matrix with better LDI efficiency for the detection of complex biological samples (plasma) metabolites

Method used

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  • Preparation method of porous alloy nano material and application of porous alloy nano material in plasma metabolite detection
  • Preparation method of porous alloy nano material and application of porous alloy nano material in plasma metabolite detection
  • Preparation method of porous alloy nano material and application of porous alloy nano material in plasma metabolite detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Preparation of Example 1 Porous Alloy Nanomaterial

[0034] Step 1: Preparation of instruments and reagents: matrix-assisted laser desorption ionization mass spectrometry, using positive ion reflectance mode;

[0035] Step 2: The preparation of the porous PdPtAu alloy includes the following steps;

[0036] Step 2.1: Dissolve 0.6mL Na 2 PdCl 4 (20mM), 3mL H 2 PtCl 6 ·6H 2 O (20mM), 60μL of hydrochloric acid (6.0M) and 60mg of F127 were mixed thoroughly, and dissolved by ultrasonication. After the F127 was completely dissolved, 3mL of ascorbic acid solution (0.1M) was added, and immediately placed in a water bath at 45°C for ultrasonic reaction for 3 hours, and 1.2mL of HAuCl was added respectively. 4 4H 2 O solution (concentrations are 10mM, 20mM, 30mM, 40mM, respectively), react for 1h. After the reaction, wash with absolute ethanol and water centrifuge (10,000 rpm) three times, and dry at 50°C for later use.

[0037] Step 2.2: Disperse the above porous PdPtAu a...

Embodiment 2

[0045] Example 2 Detection of Standard Small Molecule Substances

[0046] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization mass spectrometry, using cationic reflection mode detection; the porous alloy is prepared into a suspension, and the standard small molecule (glucose, phenylalanine, lysine) solution is prepared;

[0047] (2) Sample preparation is carried out on the mass spectrometer target plate, and dried at room temperature;

[0048] (3) detect under the mass spectrometer, and analyze the mass spectrum image, the result is as follows image 3 as shown, image 3 a, 3b, and 3c are standard small molecule solutions of glucose, phenylalanine, and lysine, respectively.

Embodiment 3

[0049] Example 3 Detection of Small Molecular Substances Containing NaCl and Bovine Serum Albumin

[0050] (1) Preparation of instruments and reagents: matrix-assisted laser desorption ionization mass spectrometry, using positive ion reflection mode detection; preparing porous alloys into suspensions, and preparing mixed small molecules (glucose, phenylalanine, lysine, etc.) with high concentrations of salt or protein acid) solution;

[0051] (2) Sample preparation is carried out on the mass spectrometer target plate, and dried at room temperature;

[0052] (3) detect under the mass spectrometer, and analyze the mass spectrum image, the result is as follows Figure 4 as shown, Figure 4 a is the mass spectrum for the detection of glucose, phenylalanine and lysine in a solution containing NaCl, Figure 4 b Mass spectra of glucose, phenylalanine and lysine detected in solution of bovine serum albumin.

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Abstract

The invention discloses a preparation method of a porous alloy nano material and application of the porous alloy nano material in plasma metabolite detection, and relates to the field of porous alloy nano material synthesis. The preparation method comprises the following steps: fully mixing Na2PdCl4, H2PtCl6*6H2O, hydrochloric acid and F127, and carrying out ultrasonic dissolution; after complete dissolution, adding an ascorbic acid solution, and immediately placing the solution in a water bath for ultrasonic reaction; then, adding a HAuCl4*4H2O solution for a reaction; and finally, centrifugally washing with absolute ethyl alcohol and water respectively, and drying. The porous alloy nano material is used as a matrix of matrix-assisted laser desorption ionization mass spectrometry to be applied to small molecule detection. The material can be synthesized through a one-step method, the steps are simple, and the cost is low; as a matrix of laser desorption ionization mass spectrometry, the material can solve the problems of hot spot effect of a traditional organic matrix and interference in a low molecular weight region; a plasma sample only needs to be simply pretreated, the accuracy rate is high, the cost is low, and the detection flux is high.

Description

technical field [0001] The invention relates to the field of synthesis of porous alloy nanomaterials, in particular to a preparation method of a porous alloy and its application in detecting plasma metabolites. Background technique [0002] The detection of biomarkers (proteins, nucleic acids, metabolites, etc.) is playing an increasingly important role in in vitro diagnosis due to its non-invasive characteristics. Unlike genes whose functions are regulated by epigenetics and proteins that are post-translationally modified, metabolites are direct markers of biochemical activity and are more easily correlated with phenotypes. Therefore, metabolite profiling, or metabolomics, has become a powerful tool that is increasingly used in in vitro diagnostics. At present, biochemical analysis and gas chromatography / liquid chromatography mass spectrometry (GC / LC MS) are the main means of metabolite detection. After pretreatment such as concentration and concentration, the detection o...

Claims

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Application Information

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IPC IPC(8): G01N27/64G01N1/28
CPCG01N27/64G01N1/28
Inventor 钱昆苏海洋黄琳
Owner SHANGHAI JIAO TONG UNIV
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