Preparation method of desalted cartilage oligopeptide powder

An oligopeptide and cartilage technology, applied in the field of preparation of desalted cartilage oligopeptide powder, can solve problems such as high consumption of pure water, and achieve the effects of reducing sewage discharge, simple operation process and improving product value.

Pending Publication Date: 2021-11-12
山东广昊生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN1392155A has reported that nanofiltration technology is applied to the desalination and concentration of animal and plant oligopeptide so...

Method used

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  • Preparation method of desalted cartilage oligopeptide powder
  • Preparation method of desalted cartilage oligopeptide powder
  • Preparation method of desalted cartilage oligopeptide powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Accurately weigh 300g of porcine cartilage and 600g of porcine trachea, wash off the surface dirt with water, then add water for continuous cooking for 5 hours, cool down to 52°C, add 30g of porcine pancreas, and add 2g of NaOH several times to adjust the pH to 8.50-9.00 , maintaining the temperature at 52 °C for 2.5 h. Weigh 1.70g alkaline protease for the second enzymolysis, add 2g NaOH several times to adjust the pH to 8.50-9.00, keep the pH in this range throughout the enzymolysis process, keep the temperature at 52°C, and enzymolysis for 5 hours, Then after inactivation and filtration, it enters the ultrafiltration membrane equipment with a molecular weight cut-off of 5000Da, so that the chondroitin sulfate and oligopeptide solution can be effectively separated, and the separated protein material liquid enters the polyamide nanofiltration membrane equipment with a molecular weight cut-off of 150-300Da. Concentrate the oligopeptide solution to the minimum volume and...

Embodiment 2

[0036] Accurately weigh 350g of chicken cartilage and 650g of duck trachea, wash off the surface dirt with water, then add water to continue cooking for 4-6 hours, cool down to 48°C, add 28g of pig pancreas, and continue to stir at 120rpm, adding in several times 2.5 g NaOH adjusted the pH to 8.50-9.00 and kept the temperature at 48 °C for 2 h. Weigh 1.50g of alkaline protease for the second enzymolysis, adjust the pH to 8.50-9.00, keep the pH in this range throughout the enzymolysis process, keep stirring during the enzymolysis process, so that the enzymolysis is sufficient and the process temperature is always Keep at about 50°C, enzymatically hydrolyze for 4 hours, then stop stirring, inactivate and filter the feed liquid into the ultrafiltration membrane equipment with a molecular weight cut-off of 5000Da, so that the chondroitin sulfate and oligopeptide solution can be effectively separated, and the separated protein feed liquid Enter the polyethersulfone nanofiltration m...

Embodiment 3

[0038] Accurately weigh 450g of duck cartilage and 550g of pig trachea, wash off the surface dirt with water, then add water for continuous cooking for 3-5 hours, cool down to 50°C, add 30g of pig pancreas, and add 3 g of NaOH several times to adjust the pH to 8.50-9.00, keep the temperature at 50°C for 2h. Weigh 1.85 g of alkaline protease for the second enzymolysis, adjust the pH to 8.50-9.00, keep the pH in this range throughout the enzymolysis process, keep the temperature at 50°C, enzymolysis for 4 hours, add 17 g of hydrochloric acid, hydrogen peroxide 3.00g, inactivated, filtered through a double-layer 400-mesh filter, the feed liquid enters the ultrafiltration membrane equipment with a molecular weight cut-off of 4000Da, so that the chondroitin sulfate and oligopeptide solution can be effectively separated, and the separated protein feed liquid enters the molecular weight cut-off of 200Da The cellulose acetate nanofiltration membrane equipment is used to concentrate th...

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Abstract

The invention relates to a preparation method of desalted cartilage oligopeptide powder. The preparation method comprises the following main processes of: taking meat poultry tracheas and cartilage as raw materials, performing cooking, enzymolysis and the like to obtain enzymatic hydrolysate, performing ultrafiltration separation to obtain chondroitin sulfate and oligopeptide liquid on an interception side and a permeation side respectively, and enabling the oligopeptide liquid to enter nanofiltration membrane equipment for desalting and pre-concentration treatment; firstly, concentrating a raw material solution by 3-5 times by adopting the nanofiltration membrane equipment, then performing continuous intermittent nanofiltration constant-volume desalting, namely adding pure water to maintain the volume in a tank body to be constant for percolation, and stopping operation until the conductivity of permeation liquid is lower than 100 microseconds/cm; then pumping nanofiltration membrane permeation liquid into reverse osmosis membrane equipment, and recycling water prepared by reverse osmosis in a nanofiltration dialysis stage; and performing vacuum evaporation concentration and spray drying on a nanofiltration membrane concentrated solution to obtain desalted oligopeptide powder, and recycling evaporated water obtained by evaporation concentration in a cooking stage. Oligopeptide prepared by the preparation method disclosed by the invention is active oligopeptide with the molecular weight lower than 2000 Da; the preparation method has better decolorization and odor removal effects, and the ash content of the oligopeptide powder is lower than 2 percent; and the preparation method has the advantages of water conservation, less sewage discharge, low cost and high quality of the oligopeptide powder.

Description

[0001] field of invention [0002] The invention relates to the field of biotechnology, in particular to a preparation method of desalted cartilage oligopeptide powder. Background technique [0003] Proteins are firstly hydrolyzed to obtain peptides, and then further enzymolyzed to obtain free amino acids. In the past, it was believed that proteins were hydrolyzed by proteases into free amino acids and finally absorbed by the human body. Some recent studies have shown that not all proteins are absorbed in the form of amino acids. Most of them are absorbed in the form of peptides, therefore, oligopeptides have some special biological effects and nutritional value. In recent years, studies have discovered some peptides that can regulate human physiological functions, enhance and exert human physiological activities: antibacterial active peptides (antibacterial peptide D, nisin, rubberin screened in silkworms), anticancer peptides (such as tumor cell necrosis Factors, cyclohexid...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 赵黎明高猛峰李洁张照照张兴兴
Owner 山东广昊生物制品有限公司
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