Small molecule fluorescent probes for aluminum ion detection and cell imaging and their applications
A cell imaging and fluorescent probe technology, applied in the detection field, can solve the problems of small toxic side effects, high toxicity, large molecular weight, etc., and achieve the effect of small toxic side effects, small detection limit, and rapid colorimetric judgment
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Embodiment 1
[0069] A small molecule fluorescent probe for aluminum ion detection and cell imaging, the small molecule fluorescent probe is 2,5-dihydroxyterephthalic acid (abbreviated as DB), and its molecular formula is: C 8 H 6 O 6 , the structural formula is:
[0070]
[0071] The small molecule fluorescent probe for aluminum ion detection and cell imaging, under ultraviolet light irradiation, DB emits yellow fluorescence, adding Al 3+ Post-fluorescence is shown in green, and Al 3+ The higher the concentration, the more obvious the green fluorescence;
[0072] The small molecule fluorescent probe used for aluminum ion detection and cell imaging, in the fluorescence spectrum, DB is in the presence of Al. 3+ , the fluorescence intensity is significantly enhanced, accompanied by a blue-shift phenomenon, and with the Al 3+ As the concentration increases, the fluorescence intensity increases continuously;
[0073] The small molecule fluorescent probe for aluminum ion detection and c...
Embodiment 2
[0076] A test paper detection method for a small molecule fluorescent probe used for aluminum ion detection and cell imaging, comprising the following steps:
[0077] 1) Cut the qualitative filter paper into strips the size of pH test paper;
[0078] 2) Put the cut filter paper in the previous step into 2×10 -4 Soak and soak in mol / L DB aqueous solution to ensure that the DB probe compound is attached to the filter paper to detect aluminum ions;
[0079] 3) Take out the filter paper soaked or sprayed in the previous step with tweezers, and place it in the air to dry naturally;
[0080] 4) Put 1.0×10 -8 mol / L-1.0×10 -3 The solution to be tested with different aluminum ion concentrations in mol / L was sprayed onto the DB test paper obtained in the previous step, and placed under 365nm ultraviolet light to observe the color change, and the color changed from yellow to bright green.
Embodiment 3
[0082] A test paper detection method for a small molecule fluorescent probe used for aluminum ion detection and cell imaging, comprising the following steps:
[0083] 1) Cut the quantitative filter paper into strips the size of pH test paper;
[0084] 2) Spray 2×10 directly on the filter paper cut in the previous step -4 The mol / L DB aqueous solution is soaked to ensure that the DB probe compound is attached to the filter paper to detect aluminum ions;
[0085] 3) The filter paper soaked or sprayed in the previous step is taken out with tweezers, placed in a vacuum drying box for 30-60min, the temperature is 45°C, and the DB test paper is made after drying;
[0086] 4) Put 1.0×10 -8 mol / L-1.0×10 -3 The solution to be tested with different aluminum ion concentrations in mol / L was dropped onto the DB test paper obtained in the previous step, and placed under 365nm ultraviolet light to observe the color change, and the color changed from yellow to bright green.
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